View source for 9nqj


==Cryo-EM structure of a bacterial prototype ATP-binding cassette transporter MalFGK2.==
<StructureSection load='9nqj' size='340' side='right'caption='[[9nqj]], [[Resolution|resolution]] 3.34&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9nqj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NQJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NQJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.34&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9nqj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9nqj OCA], [https://pdbe.org/9nqj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9nqj RCSB], [https://www.ebi.ac.uk/pdbsum/9nqj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9nqj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MALK_ECOLI MALK_ECOLI] Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Responsible for energy coupling to the transport system.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Membrane scaffold protein-based nanodiscs have facilitated unprecedented structural and biophysical analysis of membrane proteins in a near-native lipid environment. However, successful reconstitution of membrane proteins in nanodiscs requires prior solubilization and purification in detergents, which may impact their physiological structure and function. Furthermore, the detergent-mediated reconstitution of nanodiscs is unlikely to recapitulate the precise composition or asymmetry of native membranes. To circumvent this fundamental limitation of traditional nanodisc technology, we herein describe the development of membrane-solubilizing peptides to directly extract membrane proteins from native cell membranes into nanoscale discoids. By systematically protein engineering and screening, we create a class of chemically modified Apolipoprotein-A1 mimetic peptides to enable the formation of detergent-free nanodiscs with high efficiency. Nanodiscs generated with these engineered membrane scaffold peptides are suitable for obtaining high-resolution structures using single-particle cryo-EM with native lipids. To further highlight the versatility of our approach, we directly extract a sampling of membrane signaling proteins with their surrounding native membranes for biochemical and biophysical interrogations.

DeFrND: detergent-free reconstitution into native nanodiscs with designer membrane scaffold peptides.,Ren Q, Wang J, Idikuda V, Zhang S, Shin J, Ludlam WG, Real Hernandez LM, Zdancewicz S, Kreutzberger AJB, Chang H, Kiessling V, Tamm LK, Jomaa A, Levental I, Martemyanov K, Chanda B, Bao H Nat Commun. 2025 Aug 26;16(1):7973. doi: 10.1038/s41467-025-63275-8. PMID:40858559<ref>PMID:40858559</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9nqj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Baron C]]
[[Category: Huan B]]
[[Category: IIya L]]
[[Category: Jeehae S]]
[[Category: Jing W]]
[[Category: Jong HS]]
[[Category: Kirill M]]
[[Category: Luis MRH]]
[[Category: Qian R]]
[[Category: Shanwen Z]]
[[Category: Vinay I]]
[[Category: William GL]]
[[Category: Young AG]]