View source for 9ntn


==Structure of Cap10-CdnD complex containing NDG modification==
<StructureSection load='9ntn' size='340' side='right'caption='[[9ntn]], [[Resolution|resolution]] 2.43&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ntn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hyphomicrobiales Hyphomicrobiales]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NTN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2KA:2-AMINO-4-OXO-4,7-DIHYDRO-3H-PYRROLO[2,3-D]PYRIMIDINE-5-CARBOXYLIC+ACID'>2KA</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ntn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ntn OCA], [https://pdbe.org/9ntn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ntn RCSB], [https://www.ebi.ac.uk/pdbsum/9ntn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ntn ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
7-deazapurines are nucleobase analogs essential for nucleic acid modifications in nearly all cellular life. Here, we discover a role for 7-deazapurines in protein modification within type IV CBASS anti-phage defense and define functions for CBASS ancillary proteins Cap9 and Cap10 in nucleobase-protein conjugation. A structure of Cap10 reveals a tRNA transglycosylase-family enzyme remodeled to bind the modified N-terminus of a partner cGAS/DncV-like nucleotidyltransferase linked to a 7-amido-7-deazaguanine (NDG) nucleobase. The structure of Cap9 explains how this QueC-like enzyme co-opts a 7-deazapurine biosynthetic reaction mechanism for NDG conjugation. We demonstrate that Cap9, Cap10, and NDG conjugation are essential for host defense against phage infection. Our results define a previously unknown 7-deazapurine protein modification and explain how nucleobase biosynthetic machinery has been repurposed for antiviral immunity.

Deazaguanylation is a nucleobase-protein conjugation required for type IV CBASS immunity.,Wassarman DR, Pfaff P, Paulo JA, Gygi SP, Shokat KM, Kranzusch PJ bioRxiv [Preprint]. 2025 Apr 6:2025.04.06.647259. doi: 10.1101/2025.04.06.647259. PMID:40236162<ref>PMID:40236162</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9ntn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hyphomicrobiales]]
[[Category: Large Structures]]
[[Category: Gygi SP]]
[[Category: Kranzusch PJ]]
[[Category: Paulo JA]]
[[Category: Pfaff P]]
[[Category: Shokat KM]]
[[Category: Wassarman DR]]