View source for 9pjf


==GII.4 Human Norovirus 3CL protease bound to compound 27==
<StructureSection load='9pjf' size='340' side='right'caption='[[9pjf]], [[Resolution|resolution]] 1.47&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9pjf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Norovirus Norovirus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9PJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9PJF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.47&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1C3V:N-(tert-butoxycarbonyl)-L-phenylalanine'>A1C3V</scene>, <scene name='pdbligand=ALC:2-AMINO-3-CYCLOHEXYL-PROPIONIC+ACID'>ALC</scene>, <scene name='pdbligand=ELL:(2~{S})-2-azanyl-3-[(3~{S})-2-oxidanylidenepyrrolidin-3-yl]propanal'>ELL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9pjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9pjf OCA], [https://pdbe.org/9pjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9pjf RCSB], [https://www.ebi.ac.uk/pdbsum/9pjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9pjf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/K4L8Z7_9CALI K4L8Z7_9CALI] 3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave polyadenylate-binding protein thereby inhibiting cellular translation.[PROSITE-ProRule:PRU00870]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The cysteine 3C-like proteases (3CL(pro)) of caliciviruses, coronaviruses, and picornaviruses are essential for viral replication. In this study, we report the development of potent broad-spectrum peptidomimetic antiviral agents that target the 3CL(pro) of caliciviruses (NS6), coronaviruses (M(pro)), and a picornavirus (3C). Based upon previously reported inhibitors, a small library of compounds was designed, synthesized and tested to identify a core structure, which was then derivatized with a focus upon P3 and P4 positions to afford new inhibitors with improved potency against the respective viral enzymes and enhanced binding as determined by X-ray crystallography. These compounds were tested against a range of viruses in culture, revealing minimal toxicity while exhibiting broad-spectrum potent nanomolar activities against noroviruses and several coronavirus species, including alpha and omicron variants of SARS-CoV-2 and Middle East Respiratory Syndrome virus (MERS).

Broad-Spectrum Peptidomimetic Inhibitors of Norovirus and Coronavirus 3C-like Proteases.,Brimble MA, Stubbing LA, Hermant YO, Yang SH, Hubert JG, Pearl ES, McSweeney AM, Young VL, Campbell AC, Opel-Reading HK, McKenzie-Goldsmith GM, Putha L, Mortuza R, Wang H, Hurst BL, Julander J, Harris LD, Krause KL, Ward VK ACS Infect Dis. 2025 Dec 18. doi: 10.1021/acsinfecdis.5c00680. PMID:41412823<ref>PMID:41412823</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9pjf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Norovirus]]
[[Category: Synthetic construct]]
[[Category: Campbell AC]]
[[Category: Krause KL]]
[[Category: Opel-Reading HK]]