View source for 9pru


==Complex of the 3G8 Fab bound to Fc gamma receptor 3a / CD16a F158 allotype==
<StructureSection load='9pru' size='340' side='right'caption='[[9pru]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9pru]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9PRU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9PRU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9pru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9pru OCA], [https://pdbe.org/9pru PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9pru RCSB], [https://www.ebi.ac.uk/pdbsum/9pru PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9pru ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/FCG3A_HUMAN FCG3A_HUMAN] The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:23006327</ref> <ref>PMID:8608639</ref> <ref>PMID:8609432</ref> <ref>PMID:8874200</ref> 
== Function ==
[https://www.uniprot.org/uniprot/FCG3A_HUMAN FCG3A_HUMAN] Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.<ref>PMID:21768335</ref> <ref>PMID:22023369</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The affinity of Fc gamma receptor IIIa (FcgammaRIIIa) binding to immunoglobulin G1 (IgG1) correlates with patient responses for antibody-based therapeutics. Among multiple factors affecting affinity, a mechanism defining how the composition of the FcgammaRIIIa N162 glycan regulates affinity remains undefined. Here, we evaluate the binding modes of two competitive FcgammaRIIIa ligands. IgG1 Fc binding is sensitive to N162 glycan composition, unlike the antigen-binding fragment (Fab) of the FcgammaRIII-specific antibody 3G8. Both ligands bound to overlapping surfaces, utilizing different angles of attack such that the IgG1 Fc but not 3G8 Fab limited the space available to the FcgammaRIII N162 N-glycan. FcgammaRIII binding to IgG1 Fc generated a 2.1 kcal/mol penalty from a loss of N162 glycan conformational entropy, greater than the 0.3 kcal/mol penalty for 3G8 and consistent with binding measurements. Thus, the conformational entropy of the FcgammaRIIIa N162-glycan is the predominant force modulating differential binding affinity compared to 3G8 Fab binding for endogenous FcgammaRIIIa glycoforms.

The impact of N-glycan conformational entropy on the binding affinity of Fc gamma receptor IIIa/CD16a.,Kremer PG, Tolbert WD, Gazaway E, Hernandez BG, Korzeniowski MK, Dyba ZA, Grelsson T, Grant OC, Lanzilotta WN, Pazgier M, Woods RJ, Barb AW Structure. 2025 Dec 19:S0969-2126(25)00475-7. doi: 10.1016/j.str.2025.11.015. PMID:41421343<ref>PMID:41421343</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9pru" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Barb AW]]
[[Category: Kremer PG]]
[[Category: Lanzilotta WN]]