View source for 9qce


==HA70-HA17-HA33 complex from Clostridium botulinum Serotype B1==
<StructureSection load='9qce' size='340' side='right'caption='[[9qce]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9qce]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9QCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9QCE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9qce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9qce OCA], [https://pdbe.org/9qce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9qce RCSB], [https://www.ebi.ac.uk/pdbsum/9qce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9qce ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B1INQ0_CLOBK B1INQ0_CLOBK] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Botulinum neurotoxin serotype B1 (BoNT/B) is a highly potent neurotoxin and therapeutic agent. Here, we present the structure of the complete 14-subunit (780 kDa) progenitor toxin complex (L-PTC) and of five subcomplexes. The structures show how the toxin interacts with its associated components in their role to protect and deliver BoNT/B across epithelial barriers. Each subcomplex, including the M-PTC, M-PTC-HA70, NTNH-HA70, and HA70 trimer, provides detailed understanding of the assembly mechanism, in which the NTNH-nLoop adopts a unique fold that locks the M-PTC into a central pore formed by HA70. The HA subcomplex presents a tripod architecture with flexible legs that may adapt to the rugged cell surface. Mass photometry reveals the pH dependence of BoNT/B release from the complex which is unexpectedly influenced by the presence of HA70. This study provides the complete L-PTC structure, offering insights into its assemblage and supporting the development of countermeasures and therapeutic applications.

Structure of the complete 14-subunit botulinum neurotoxin B complex reveals a unique anchoring through the narrow central pore of HA70.,Krc A, Kosenina SP, Nowakowska MB, Masuyer G, Stenmark P Sci Adv. 2025 Aug 29;11(35):eadx5058. doi: 10.1126/sciadv.adx5058. Epub 2025 Aug , 27. PMID:40864696<ref>PMID:40864696</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9qce" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Clostridium botulinum]]
[[Category: Large Structures]]
[[Category: Krc A]]
[[Category: Masuyer G]]
[[Category: Persson Kosenina S]]
[[Category: Stenmark P]]