View source for 9qci


==Crystal structure of PhoC mutant acid phosphatase==
<StructureSection load='9qci' size='340' side='right'caption='[[9qci]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9qci]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Morganella_morganii Morganella morganii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9QCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9QCI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9qci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9qci OCA], [https://pdbe.org/9qci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9qci RCSB], [https://www.ebi.ac.uk/pdbsum/9qci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9qci ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHOC_MORMO PHOC_MORMO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The rapid emergence of RNA therapeutics has highlighted the need for more efficient, scalable and sustainable methods for their manufacture. Biocatalytic approaches hold particular promise, but rely on a secure, sustainable and low-cost supply of nucleoside triphosphate (NTP) building blocks, including those containing chemical modifications. Here we report the development of a biocatalytic approach and engineered enzymes to convert widely available nucleosides into NTPs featuring pharmaceutically relevant modifications using inexpensive phosphate donors. Importantly our strategy obviates the need for ATP as a phosphate donor that complicates NTP isolation using existing methods. To showcase the utility of our approach, we employ an engineered acid phosphatase, polyphosphate kinase and acetate kinase to produce 2'-O-methoxyethyl-ATP (2'-MOE-ATP) and 2'-fluoro-ATP, key building blocks of commercial therapeutics. Finally, we show that crude NTPs from our process can be used directly in enzymatic oligonucleotide synthesis, obviating the need for costly NTP isolation or purification steps.

Enzymatic synthesis of key RNA therapeutic building blocks using simple phosphate donors.,Meng Q, Benckendorff C, Morrill C, Zhuo Y, Egerstrom A, Ni Cheallaigh A, Derrington SR, Obexer R, Ortmayer M, Levy CW, Finnigan JD, Charnock SJ, Turner NJ, Miller GJ, Lovelock SL Nat Commun. 2025 Dec 17. doi: 10.1038/s41467-025-67366-4. PMID:41402308<ref>PMID:41402308</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9qci" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Morganella morganii]]
[[Category: Levy CW]]
[[Category: Ortmayer M]]