View source for 9r10


==Cryo-EM structure of human MATE1 in complex with cimetidine==
<StructureSection load='9r10' size='340' side='right'caption='[[9r10]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9r10]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9R10 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9R10 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=SD9:2-cyano-1-methyl-3-[2-[(5-methyl-1~{H}-imidazol-4-yl)methylsulfanyl]ethyl]guanidine'>SD9</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9r10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9r10 OCA], [https://pdbe.org/9r10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9r10 RCSB], [https://www.ebi.ac.uk/pdbsum/9r10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9r10 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/S47A1_HUMAN S47A1_HUMAN] Multidrug efflux pump that functions as a H(+)/organic cation antiporter (PubMed:16330770, PubMed:17509534). Plays a physiological role in the excretion of cationic compounds including endogenous metabolites, drugs, toxins through the kidney and liver, into urine and bile respectively (PubMed:16330770, PubMed:17495125, PubMed:17509534, PubMed:17582384, PubMed:18305230, PubMed:19158817, PubMed:21128598, PubMed:24961373). Mediates the efflux of endogenous compounds such as creatinine, vitamin B1/thiamine, agmatine and estrone-3-sulfate (PubMed:16330770, PubMed:17495125, PubMed:17509534, PubMed:17582384, PubMed:18305230, PubMed:19158817, PubMed:21128598, PubMed:24961373). May also contribute to regulate the transport of cationic compounds in testis across the blood-testis-barrier (Probable).<ref>PMID:16330770</ref> <ref>PMID:17495125</ref> <ref>PMID:17509534</ref> <ref>PMID:17582384</ref> <ref>PMID:18305230</ref> <ref>PMID:19158817</ref> <ref>PMID:21128598</ref> <ref>PMID:24961373</ref> <ref>PMID:35307651</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human MATE1 (multidrug and toxin extrusion protein 1) is highly expressed in the kidney and liver, where it mediates the final step in the excretion of a broad range of cationic drugs, including the antidiabetic drug metformin, into the urine and bile. This transport process is essential for drug clearance and also affects therapeutic efficacy. To understand the molecular basis of drug recognition by hMATE1, we determined cryo-electron microscopy structures of the transporter in complex with the substrates 1-methyl-4-phenylpyridinium (MPP) and metformin and with the inhibitor cimetidine. The structures reveal a shared binding site located in a negatively charged pocket in the C-lobe of the protein. We functionally validated key interactions using radioactivity-based cellular uptake assays using hMATE1 mutants. Molecular dynamics simulations provide insights into the different binding modes and dynamic behaviour of the ligands within the pocket. Collectively, these findings define the structural basis of hMATE1 substrate specificity and shed light on its role in drug transport and drug-drug interactions.

Structural basis of drug recognition by human MATE1 transporter.,Romane K, Peteani G, Mukherjee S, Kowal J, Rossi L, Hou J, Kossiakoff AA, Lemmin T, Locher KP Nat Commun. 2025 Oct 27;16(1):9444. doi: 10.1038/s41467-025-64490-z. PMID:41145429<ref>PMID:41145429</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9r10" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Hou J]]
[[Category: Kossiakoff A]]
[[Category: Kowal J]]
[[Category: Lemmin T]]
[[Category: Locher KP]]
[[Category: Mukherjee S]]
[[Category: Peteani G]]
[[Category: Romane K]]
[[Category: Rossi L]]