View source for 9rxh


==Cytochrome P450 decarboxylase from Staphylococcus aureus (OleT_Sa) with elaidic acid and acetate bound==
<StructureSection load='9rxh' size='340' side='right'caption='[[9rxh]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9rxh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9RXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9RXH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ELA:9-OCTADECENOIC+ACID'>ELA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9rxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9rxh OCA], [https://pdbe.org/9rxh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9rxh RCSB], [https://www.ebi.ac.uk/pdbsum/9rxh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9rxh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A657XIU7_MAMSC A0A657XIU7_MAMSC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Certain members of the bacterial cytochrome P450 152 family (CYP152) are peroxygenases that catalyse the decarboxylation of fatty acids into terminal olefins making them attractive biocatalysts for biofuel production. To date, the characterisation of decarboxylating CYP152s has mainly focused on their reaction with saturated fatty acid substrates. CYP152s are often co-purified with a bound substrate, which is generally removed before further experiments are conducted. In the present work we identified that heterologous over-expressed CYP152 from Staphylococcus aureus (OleT(Sa)) is co-purified with the trans-monounsaturated C(18:1) fatty acid, elaidic acid. We report the spectral, thermodynamic and kinetic characteristics of OleT(Sa) bound to both elaidic acid and its saturated counterpart, stearic acid. Despite differing spectral profiles, metabolic and kinetic studies reveal that OleT(Sa) is capable of decarboxylating elaidic acid, converting it to heptadeca-1,8-diene following addition of hydrogen peroxide, at the same rate and chemoselectivity as the conversion of stearic acid to 1-heptadecane. The X-ray crystal structure of the as purified OleT(Sa) in complex with elaidic acid is also presented, allowing for several key residues to be identified for site-directed mutagenesis studies. The influence of the site-directed variants on C(18:0) and C(18:1) product formation, binding thermodynamics and kinetics have been investigated, showing that while spectral differences occur as a likely result of perturbing the binding pocket, this does not alter the chemoselectivity of the enzyme. Our work provides important insights into the mechanism of decarboxylation of an unsaturated fatty acid substrate by OleT(Sa) potentially expanding the sustainable substrate space available for CYP152s.

The cytochrome P450 decarboxylase from Staphylococcus aureus can produce a diene from a C18 monounsaturated fatty acid: A spectroscopic, structural and kinetic characterisation.,Williams LJ, Wilson MT, Birrell JA, Lang HW, Bell SG, Worrall JAR J Inorg Biochem. 2025 Oct 16;275:113117. doi: 10.1016/j.jinorgbio.2025.113117. PMID:41125001<ref>PMID:41125001</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9rxh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Williams LJ]]
[[Category: Worrall JAR]]