View source for 9ski


==Polysaccharide co-polymerase FepE open 7 subunit complex==
<StructureSection load='9ski' size='340' side='right'caption='[[9ski]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ski]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9SKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9SKI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ski FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ski OCA], [https://pdbe.org/9ski PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ski RCSB], [https://www.ebi.ac.uk/pdbsum/9ski PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ski ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FEPE_ECOLI FEPE_ECOLI] Part of the ferric enterobactin transport system.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lipopolysaccharides are important components of the gram-negative bacterial cell envelope that are involved in immune evasion and act as a protective barrier. Employing cryo-electron microscopy, we resolved the structure and dynamics of FepE, the copolymerase component of the Wzy-dependent pathway, responsible for the length modulation of very long O-antigen molecules. Comparison of the interior volumes of related copolymerases' periplasmic domains with the volume of hydrated sugars suggests that the size of the periplasmic domain controls the length of the O-antigen, implying that polysaccharide chain polymerization occurs inside the copolymerase periplasmic domain. Moreover, we show the opening of the FepE complex as well as other large mechanistically relevant movements. The opening of the complex presents an attractive corridor for the release of completed polysaccharide chains.

Structural Basis of Lipopolysaccharide O-Antigen Chain Length Modality.,Wiseman B, Widmalm G, Hogbom M Research (Wash D C). 2026 May 12;9:1276. doi: 10.34133/research.1276. eCollection , 2026. PMID:42131584<ref>PMID:42131584</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9ski" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Hogbom M]]
[[Category: Wiseman B]]