View source for 9t4k


==Human Diphosphoinositol Polyphosphate Phosphohydrolase 1 (DIPP1) H91M mutant in complex with IP6==
<StructureSection load='9t4k' size='340' side='right'caption='[[9t4k]], [[Resolution|resolution]] 1.39&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9t4k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9T4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9T4K FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.394&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9t4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9t4k OCA], [https://pdbe.org/9t4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9t4k RCSB], [https://www.ebi.ac.uk/pdbsum/9t4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9t4k ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NUDT3_HUMAN NUDT3_HUMAN] Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate), suggesting that it may play a role in signal transduction. InsP6 (inositol hexakisphophate) is not a substrate. Acts as a negative regulator of the ERK1/2 pathway. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate.<ref>PMID:9822604</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Diphosphoinositol Polyphosphate Phosphohydrolase 1 (DIPP1) is a Nudix hydrolase involved in inositol pyrophosphate (PP-InsP) metabolism, critical for cellular signaling, energy homeostasis, and stress responses. We report crystallographic and computational studies that reveal 1,5-bis-diphosphoinositol tetrakisphosphate (IP(8)) binds to DIPP1 in two catalytically-productive inositol ring conformations. IP(8) hydrolysis at the 1-position requires a twist-boat conformation, whereas at the 5-position a canonical chair conformation is adopted. Additionally, structural and biophysical characterization shows that the DIPP1 family undergoes ligand-sensitive changes in the association state that might be further modulated by salt concentration and/or phosphate ions. Taken together, these results advance our understanding of DIPP1 in the dynamic regulation of inositol pyrophosphate signaling networks. They provide a detailed view of DIPP1 substrate recognition and suggest oligomerization as a novel regulatory mechanism, with broader implications for phosphate sensing and functional protein-protein interactions.

The DIPP1 family binds IP(8) in catalytically-productive twist-boat and chair conformations and associates in a ligand-dependent manner.,Casas-Florez D, Whitfield H, Perez-Canadillas JM, Monterroso B, Riley AM, Marquez-Monino MA, Shipton ML, Sanz-Aparicio J, Brearley CA, Potter BVL, Gonzalez B Int J Biol Macromol. 2026 May 26:152715. doi: 10.1016/j.ijbiomac.2026.152715. PMID:42202926<ref>PMID:42202926</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9t4k" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Casas-Florez D]]
[[Category: Gonzalez B]]
[[Category: Marquez-Monino MA]]