View source for 9taw


==Structure of the human inner kinetochore CCAN bound to DNA==
<StructureSection load='9taw' size='340' side='right'caption='[[9taw]], [[Resolution|resolution]] 3.54&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9taw]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9TAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9TAW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.54&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9taw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9taw OCA], [https://pdbe.org/9taw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9taw RCSB], [https://www.ebi.ac.uk/pdbsum/9taw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9taw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CENPH_HUMAN CENPH_HUMAN] Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.<ref>PMID:14536089</ref> <ref>PMID:16875666</ref> <ref>PMID:18007590</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Human kinetochores assemble onto centromeric DNA comprising repetitive arrays of the 171 bp alpha-satellite sequence. To understand the higher-order architecture of the inner kinetochore assembled onto alpha-satellite arrays, we show cryo-EM structures of CCAN with free DNA, and alpha-satellite repeat monomers and dimers with CENP-A nucleosomes. CCAN bound to free DNA and a monomeric CENP-A nucleosome engages 70 bp of DNA comprising 30 bp of an upstream alpha-satellite repeat. This upstream DNA interacts with the histone-fold domain subunits of the CENP-TWSX module in a manner resembling how nucleosomes wrap DNA gyres. A complex of CCAN assembled onto a dimeric alpha-satellite repeat with two CENP-A nucleosomes shows that CCAN can only be accommodated on the linker DNA by unwrapping DNA from both the CENP-TWSX module and the upstream nucleosome. We discuss the implications of these results for models of CCAN assembly on arrays of alpha-satellite chromatin containing CENP-A nucleosomes.

Models for the architecture of the human inner kinetochore on centromeric alpha-satellite CENP-A nucleosome arrays.,Yu C, Muir KW, Yang J, Zhang Z, McLaughlin SH, Barford D Nat Commun. 2026 May 12. doi: 10.1038/s41467-026-72856-0. PMID:42120391<ref>PMID:42120391</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9taw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Barford D]]
[[Category: Muir KW]]
[[Category: Yu C]]