View source for 9to1


==Room temperature serial crystal structure of CTX-M-15 class A beta-lactamase uncomplexed resting state from PAL-XFEL==
<StructureSection load='9to1' size='340' side='right'caption='[[9to1]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9to1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9TO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9TO1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9to1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9to1 OCA], [https://pdbe.org/9to1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9to1 RCSB], [https://www.ebi.ac.uk/pdbsum/9to1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9to1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G3G192_KLEPN G3G192_KLEPN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We describe the design and implementation of a drop-on-fixed-target method for time-resolved serial crystallography at both synchrotron and XFEL facilities. A piezoelectric droplet dispensing pipette is employed for addition of picolitre volume aqueous droplets ( approximately 40-90 pl; approximately 40-55 microm diameter sphere), containing (co-)substrate(s) or ligand(s), onto enzyme microcrystals previously loaded into the trapezoidal wells of an etched crystalline silicon fixed-target chip containing 25 600 wells in a high-density, square grid with 125 microm centre-to-centre well spacing. These features demand exquisite accuracy and thereby constrain motion controls to enable robust time-resolved crystallographic studies. The system was tested with three enzyme systems, comprising lysozyme and two beta-lactamases, CTX-M-15 and AmpC(EC). Mitigation strategies for cross-well contamination, including the implementation of interleaved controls, are described; the overall performance of the system at synchrotron and X-ray free-electron laser facilities was evaluated. This drop-on-fixed-target method is a reliable framework for time-resolved crystallography and will improve the consistency of measurements across facilities.

Drop-on-fixed-target reaction initiation approach for serial and time-resolved crystallography.,Kamps JJAG, Hinchliffe P, Glerup J, Freeman EI, Lang PA, Tooke CL, Beer M, Parkinson L, Gu DH, Park S, Devenish N, Zhou T, Shilova A, Kaur S, Rabe P, Schofield CJ, Spencer J, Park J, Owen RL, Orville AM, Aller P IUCrJ. 2026 Jul 1. doi: 10.1107/S2052252526003489. PMID:42246252<ref>PMID:42246252</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9to1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Klebsiella pneumoniae]]
[[Category: Large Structures]]
[[Category: Hinchliffe P]]
[[Category: Spencer J]]
[[Category: Tooke CL]]