View source for 9ugo


==Cryo-EM structure of the HBsAg dimer and Complex with Fab==
<StructureSection load='9ugo' size='340' side='right'caption='[[9ugo]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ugo]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Hepatitis_B_virus_genotype_A Hepatitis B virus genotype A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9UGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9UGO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ugo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ugo OCA], [https://pdbe.org/9ugo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ugo RCSB], [https://www.ebi.ac.uk/pdbsum/9ugo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ugo ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Plasmablast-derived HBV surface antigen (HBsAg)-specific monoclonal antibody (mAb) and structural basis for binding to native HBsAg are poorly known. OBJECTIVE: We aimed to identify plasmablast-derived HBsAg-specific mAbs, evaluate their antiviral activities and resolve their structure for binding to native HBsAg. DESIGN: A previously vaccinated volunteer was enrolled in this study, who was boosted with a dose of recombinant hepatitis B vaccine and donated the blood sample. Activated plasmablasts were sorted from fresh peripheral blood mononuclear cells and mAbs were expressed. Their gene features, cross-genotypic binding activities and antiviral functions in vitro and in vivo were comprehensively analysed. The cryo-electron microscopy (cryo-EM) was used to determine the structure of representative mAb bound to the native HBsAg. RESULTS: In this study, we cloned a series of HBsAg-specific mAbs directly from clonally expanded plasmablasts from a vaccinated individual. Most of the mAbs displayed cross-reactivities of binding to different genotype HBsAg proteins and antiviral functions such as neutralisation and antibody-dependent cellular phagocytosis. These human anti-HBsAg mAbs, especially SY-4-class and SY-23-class, could be good candidates for antibody drugs. The cryo-EM structure of SY-23 bound to the dimeric HBsAg was determined, revealing its binding mechanism and unprecedented structural detail of the major antigenic loop (AGL) of HBsAg. CONCLUSION: Overall, our work has uncovered the diverse gene features and varied anti-HBV activities of plasmablast-derived mAbs, providing a series of antibody drug candidates and the long-sought-after atomic model of AGL has paved the way for a wholistic characterisation of the AGL's dynamic conformation during HBV infection and immune response.

Characterisation of plasmablast-derived HBsAg-specific antibody and its structural basis for binding to native HBsAg dimer.,Ju B, Liu Z, Yan H, Liu Y, Zhang L, Ge X, Wang X, Si Z, Zhou B, Fan Q, Wang M, Li Y, Lai W, Gan J, Wang H, Zhao J, Xia Y, Liao M, Zhang Z Gut. 2026 Feb 9:gutjnl-2025-336641. doi: 10.1136/gutjnl-2025-336641. PMID:41663152<ref>PMID:41663152</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9ugo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Hepatitis B virus genotype A]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Ju B]]
[[Category: Liao M]]
[[Category: Liu Y]]
[[Category: Liu Z]]
[[Category: Zhang Z]]