View source for 9utw


==Structure of dimeric FKS1 in complex with tRNA==
<StructureSection load='9utw' size='340' side='right'caption='[[9utw]], [[Resolution|resolution]] 2.96&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9utw]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9UTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9UTW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.96&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=DCR:ICOSANOIC+ACID'>DCR</scene>, <scene name='pdbligand=DD9:NONANE'>DD9</scene>, <scene name='pdbligand=HP6:HEPTANE'>HP6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEF:DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE'>PEF</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=PRD_900024:beta-laminaribiose'>PRD_900024</scene>, <scene name='pdbligand=XKP:[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-octanoyloxy-propan-2-yl]+decanoate'>XKP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9utw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9utw OCA], [https://pdbe.org/9utw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9utw RCSB], [https://www.ebi.ac.uk/pdbsum/9utw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9utw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YM8V_YEAST YM8V_YEAST] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
FKS1 is a beta-1,3-glucan synthase critical for fungal cell wall formation and a target for antifungal drugs such as echinocandin and ibrexafungerp. However, the mechanisms regulating FKS1 activity remain largely unknown. Here, we reveal that transfer RNA (tRNA) acts as an endogenous inhibitor, whereas GSR1 functions as a stabilizer of FKS1. The cryo-EM structure of FKS1 adopts a tRNA-mediated homodimer configuration, representing a quiescent state of beta-1,3-glucan synthase. Unexpectedly, the copurified endogenous tRNA is identified as a potent inhibitor that suppresses FKS1 activity. Moreover, high-resolution cryo-EM density analysis enable the identification of GSR1 as an additional binding partner of FKS1. Mutagenesis experiments confirm the interaction between FKS1 and GSR1. Evolutionarily conserved GSR1 is found to increase the stability of FKS1 in beta-1,3-glucan biosynthesis. Collectively, our findings identify both tRNA and GSR1 as intrinsic modulators of beta-1,3-glucan biosynthesis, thereby providing opportunities for the further development of FKS1-targeted antifungal drugs.

Structural-guided identification of two modulators of beta-1,3-glucan synthase FKS1.,Li J, Li J, Zhu A, Dai X, Liu J, Liu H, Xia Z, Dong Y, Qian W, Dai L, Guo L, Yan C, Deng D, Luo Y, Wang X Nat Commun. 2025 Dec 7. doi: 10.1038/s41467-025-67293-4. PMID:41354657<ref>PMID:41354657</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9utw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Dai XL]]
[[Category: Deng D]]
[[Category: Li JL]]
[[Category: Liu JX]]
[[Category: Wang X]]
[[Category: Yan CY]]
[[Category: Zhu AQ]]