View source for 9vap


==Cryo-EM structure of formate dehydrogenase from Shewanella oneidensis MR-1 (SoFdhAB)==
<StructureSection load='9vap' size='340' side='right'caption='[[9vap]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9vap]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis_MR-1 Shewanella oneidensis MR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9VAP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9VAP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=W:TUNGSTEN+ION'>W</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9vap FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9vap OCA], [https://pdbe.org/9vap PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9vap RCSB], [https://www.ebi.ac.uk/pdbsum/9vap PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9vap ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bioelectrocatalytic CO(2) reduction offers a sustainable route for CO(2) bioconversion, yet remains limited by interfacial-intramolecular electron transfer and oxygen sensitivity. Here, we mine a formate dehydrogenase from Shewanella oneidensis MR-1 (SoFdhAB) featuring completely oxygen tolerant and direct-electron-transfer (DET) electrocatalytic performances. Cryo-electron microscopy (Cryo-EM) analysis reveals an intramolecular electron highway comprising five [4Fe-4S] clusters, a regional face-face contact facilitating interfacial ET, and a unique oxygen resistance mechanism different from inactivation-activation. By acquiring a beneficial variant SoFdhAB-Y94S, a direct bioelectrocatalytic CO(2) reduction system is constructed, accumulating 2.88 +/- 0.03 mmol formate in 64 hours with a steady rate of 45.3 +/- 0.5 mumol h(-1) cm(-2) and a Faradaic efficiency of 93.1 +/- 5.2%. The merits of oxygen tolerance and efficient (electro)catalytic property endow SoFdhAB a robust enzyme adopted in potential application scenarios, and the inherent DET capability may inspire the interfacial engineering of other oxidoreductases.

An interfacial-intramolecular electron highway for accelerated electrocatalytic CO(2) reduction by an O(2)-tolerant formate dehydrogenase.,Liu W, Zhang P, Wang X, Zhang K, Yang W, Cui H, Liu J, Sun J, You C, Cui H, Zhu Z, Zhang L Nat Commun. 2026 Mar 3. doi: 10.1038/s41467-026-69827-w. PMID:41775696<ref>PMID:41775696</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9vap" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Shewanella oneidensis MR-1]]
[[Category: Liu W]]
[[Category: Zhang L]]