View source for 9vcf


==Cryo-EM structure of Shewanella putrefaciens ComEC in complex with dsDNA==
<StructureSection load='9vcf' size='340' side='right'caption='[[9vcf]], [[Resolution|resolution]] 3.23&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9vcf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_putrefaciens Shewanella putrefaciens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9VCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9VCF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.23&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9vcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9vcf OCA], [https://pdbe.org/9vcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9vcf RCSB], [https://www.ebi.ac.uk/pdbsum/9vcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9vcf ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Natural transformation is one of the major pathways of horizontal gene transfer in bacteria, enabling the acquisition of extracellular DNA and its integration into the host genome. ComEC is a membrane protein responsible for DNA translocation in this process, yet its precise function and structure have remained elusive. Here, we report cryo-electron microscopy structures of ComEC in DNA-free, single-stranded DNA (ssDNA)-bound, and double-stranded DNA (dsDNA)-bound forms, together with biochemical analyses. These structures reveal that ComEC cleaves one strand of dsDNA at its extracellular domain and guides the remaining strand into a positively charged pore formed within the membrane domain. These findings provide a structural basis for the long-hypothesized roles of ComEC in both DNA processing and translocation across the inner membrane during natural transformation.

Structural basis for DNA processing and membrane translocation by ComEC in natural transformation.,Hirano H, Tsuji N, Chiba S, Nureki O Science. 2026 Apr 16;392(6795):311-316. doi: 10.1126/science.aea3485. Epub 2026 , Apr 16. PMID:41990170<ref>PMID:41990170</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Shewanella putrefaciens]]
[[Category: Synthetic construct]]
[[Category: Hirano H]]
[[Category: Nureki O]]