View source for 9vfk


==Putative BLF1-like Deamidase from Methylomonas sp. Kb3==
<StructureSection load='9vfk' size='340' side='right'caption='[[9vfk]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9vfk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylomonas_sp._Kb3 Methylomonas sp. Kb3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9VFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9VFK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9vfk OCA], [https://pdbe.org/9vfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9vfk RCSB], [https://www.ebi.ac.uk/pdbsum/9vfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9vfk ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Bacteria that act as eukaryotic pathogens are known to express enzyme toxins that interfere with critical pathways in their targets. Among these are deamidases that modify conserved glutamine residues such as those found in eukaryotic initiation factor 4A (eIF4A) or in the small GTPases Rho, Rac and Cdc42, converting them to glutamic acid and thereby disrupting the function of the target proteins. Structural and biochemical studies have shown that deamidases from Burkholderia, Burkholderia lethal factor 1 (BLF1), and from Escherichia coli, cytotoxic necrotizing factor (CNF1), share a similar fold in their deamidase domains with similar mechanisms for deamidase chemistry. The active site of these toxins contains an apparently conserved LSGC motif as part of a cysteine-histidine catalytic dyad which catalyses the site-specific deamidation. Sequence searches in bacterial databases have suggested that deamidases with this fold are more widespread in nature and that the LSGC motif varies substantially. Using crystals grown in magnesium sulfate, we determined the 1.95 A resolution crystal structure of a putative deamidase from Methylomonas sp. Kb3, CWO84_RS12955, which shows limited sequence identity to BLF1. The structure reveals that CWO84_RS12955 adopts a similar fold to that of BLF1, albeit with significant differences at the periphery due to sequence insertions and deletions, and that it conserves the catalytic dyad but replaces the LSGC motif with MDGC. However, compared with BLF1, none of the residues that interact with eIF4A are conserved. These structural features suggest that the Methylomonas protein that we have identified may act as a BLF1-like deamidase but against a currently unknown target, and that other bacterial proteins with similar sequence fingerprints could play important roles in broader bacterial biology.

Structural insights into a putative BLF1-like deamidase from Methylomonas sp. Kb3.,Muhamad Ismail NAS, Abd Aziz AA, Nakagawa A, Abu Bakar FD, Abdul Murad AM, Nathan S, Mohd Raih MF, Quay DHX, Rice D Acta Crystallogr F Struct Biol Commun. 2026 Oct 1. doi: , 10.1107/S2053230X2600868X. PMID:42745721<ref>PMID:42745721</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methylomonas sp. Kb3]]
[[Category: Abd Aziz AA]]
[[Category: Abdul Murad AM]]
[[Category: Abu Bakar FD]]
[[Category: Firdaus-Raih M]]
[[Category: Muhamad Ismail NAS]]
[[Category: Nakagawa A]]
[[Category: Nathan S]]
[[Category: Quay DHX]]
[[Category: Rice D]]