View source for 9waq


==Crystal structure of the effector-immunity VP14460-VP14465 in Vibrio parahaemolyticus==
<StructureSection load='9waq' size='340' side='right'caption='[[9waq]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9waq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_parahaemolyticus Vibrio parahaemolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WAQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9waq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9waq OCA], [https://pdbe.org/9waq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9waq RCSB], [https://www.ebi.ac.uk/pdbsum/9waq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9waq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A024B384_VIBPH A0A024B384_VIBPH] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Vibrio parahaemolyticus, a halophilic pathogen, contaminates seafood and high-salt foods, posing significant health risks such as gastroenteritis and fatalities. With increasing seafood consumption, developing effective control strategies is imperative. The type VI secretion system, a common molecular weapon in Vibrio that mediates cross-domain interactions, is a contractile nanomachine that deploys antibacterial effectors, each paired with a cognate immunity protein to form effector-immunity (E-I) modules. Here, we present the first structural and functional characterization of this complex. The crystal structure of the VP14460-VP14465 complex unveils the molecular basis for specific E-I recognition, defining a set of critical interfacial residues. Structure-guided mutagenesis confirmed that these residues are essential for complex integrity, their disruption liberates the VP14465 toxin, unleashing potent bactericidal activity. Furthermore, we identified the active sites responsible for the DNase activity of VP14465. Notably, while VP14460 and VP14465 form a stable monomeric complex in the bound state, each isolated component exhibits distinct oligomeric behavior in vitro. This suggests a dynamic assembly-disassembly mechanism that may regulate effector delivery and toxin activation during interbacterial competition. Collectively, our findings provide mechanistic insights into type VI secretion system E-I module architecture and function, and establish a structural framework for the development of novel antibacterial therapeutics targeting DNase family effectors.

Structural and mechanistic insights into the VP14460-VP14465 effector-immunity module of the Vibrio parahaemolyticus type VI secretion system.,Zheng Y, Zheng C, Ye Z, Huang L, Lin X, Wu B, Pan Z, Qiu R, Cai J, Xu L, Deng Z, Xu R, Xie X, Xie L, Hu F J Biol Chem. 2026 Jun 15;302(8):113257. doi: 10.1016/j.jbc.2026.113257. PMID:42297241<ref>PMID:42297241</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9waq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vibrio parahaemolyticus]]
[[Category: Hu F]]