View source for 9wix


==Cryo-EM structure of VGLUT3 in apo state==
<StructureSection load='9wix' size='340' side='right'caption='[[9wix]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9wix]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WIX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wix OCA], [https://pdbe.org/9wix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wix RCSB], [https://www.ebi.ac.uk/pdbsum/9wix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wix ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VGLU3_MOUSE VGLU3_MOUSE] Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, sodium and phosphate (PubMed:12384506, PubMed:18080752, PubMed:18215623). At the synaptic vesicle membrane, mainly functions as an uniporter that mediates the uptake of L-glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed:12384506, PubMed:18080752, PubMed:18215623). The L-glutamate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane (PubMed:12384506). In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification (By similarity). At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation (By similarity). The symporter activity is electrogenic (By similarity). Moreover, operates synergistically with SLC18A3/VACHT under a constant H(+) gradient, thereby allowing striatal vesicular acetylcholine uptake (PubMed:18278042).[UniProtKB:Q7TSF2][UniProtKB:Q8NDX2]<ref>PMID:12384506</ref> <ref>PMID:18080752</ref> <ref>PMID:18215623</ref> <ref>PMID:18278042</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Chao YL]]
[[Category: Qu QH]]
[[Category: Wang X]]
[[Category: Yang CH]]
[[Category: Zhou ZX]]