View source for 9wm1


==Crystal structure of Escherichia coli RecG in complex with a partial replication fork and ADPBeF3, ternary complex I==
<StructureSection load='9wm1' size='340' side='right'caption='[[9wm1]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9wm1]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Chemical_production_metagenome Chemical production metagenome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WM1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WM1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wm1 OCA], [https://pdbe.org/9wm1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wm1 RCSB], [https://www.ebi.ac.uk/pdbsum/9wm1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wm1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RECG_ECOLI RECG_ECOLI] Genetic interactions indicate that RecG or RadD are required for DNA repair in every replication cycle; they function in different pathways, each is essential in the absence of the other (PubMed:32644157). Plays a critical role in recombination and DNA repair (PubMed:8428576, PubMed:7957087, PubMed:8127666, PubMed:9265736, PubMed:10871364, PubMed:32644157). RecG or RadD are required for DNA maintenance in every replication cycle (PubMed:32644157). Helps process Holliday junction (HJ) intermediates to mature products by catalyzing branch migration (PubMed:8428576, PubMed:7957087, PubMed:8127666, PubMed:10871364). Has replication fork regression activity, unwinds stalled or blocked replication forks to make a HJ that can be resolved by RuvC or RusA (PubMed:10778854, PubMed:11459957). Also rewinds unwound dsDNA in an ATP-dependent manner (PubMed:24013402). Has double-stranded (ds)DNA unwinding activity characteristic of a DNA helicase with 3'-5' polarity in vitro on linear dsDNA; branched duplex DNA (Y-DNA) substrates adopt different conformations that influence which of the two arms are unwound (PubMed:7957087). Binds and unwinds HJ and Y-DNA but not linear duplex DNA; binds no more than 10 nucleotides of ssDNA at a fork (PubMed:8428576, PubMed:7957087, PubMed:8127666, PubMed:10871364, PubMed:11459957, PubMed:15533834). Has a role in constitutive stable DNA replication (cSDR, DNA replication in the absence of protein synthesis) and R-loop (RNA annealed with dsDNA) formation (PubMed:7774596). Unwinds R-loops but not RNA:DNA hybrids (PubMed:8980680, PubMed:15533834). Is genetically synergistic to RadA and RuvABC (PubMed:12446634, PubMed:25484163).<ref>PMID:10778854</ref> <ref>PMID:10871364</ref> <ref>PMID:11459957</ref> <ref>PMID:12446634</ref> <ref>PMID:15533834</ref> <ref>PMID:24013402</ref> <ref>PMID:25484163</ref> <ref>PMID:32644157</ref> <ref>PMID:7774596</ref> <ref>PMID:7957087</ref> <ref>PMID:8127666</ref> <ref>PMID:8428576</ref> <ref>PMID:9265736</ref>   Uup probably acts upstream of RecG; they are important in repair of stalled replication forks and postreplication gaps (PubMed:32644157). Lethal double radD-recG deletions are suppressed by a reduction in recA expression, by point mutations in priA helicase, by deletions in recF, recO or uup, suggesting all these proteins create branched DNA intermediates requiring RadD or RecG for resolution (PubMed:32644157).<ref>PMID:32644157</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Chemical production metagenome]]
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Cheng K]]