View source for 9wsw


==Cryo-EM structure of endomorphin-1-muOR-Gz-scFv16 complex==
<StructureSection load='9wsw' size='340' side='right'caption='[[9wsw]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9wsw]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WSW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WSW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wsw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wsw OCA], [https://pdbe.org/9wsw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wsw RCSB], [https://www.ebi.ac.uk/pdbsum/9wsw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wsw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GBB1_HUMAN GBB1_HUMAN] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.<ref>PMID:18611381</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Activation of the mu-opioid receptor (muOR) alleviates pain but also elicits adverse effects through diverse G proteins and beta-arrestins. The structural details of muOR complexes with G(z) and beta-arrestins have not been determined, impeding a comprehensive understanding of muOR signaling plasticity. Here, we present the cryo-EM structures of the muOR-G(z) and muOR-betaarr1 complexes, revealing selective conformational preferences of muOR when engaged with specific downstream signaling transducers. Integrated receptor pharmacology, including high-resolution structural analysis, cell signaling assays, and molecular dynamics simulations, demonstrated that transmembrane helix 1 (TM1) acts as an allosteric regulator of muOR signaling bias through differential stabilization of the G(i)-, G(z)-, and betaarr1-bound states. Mechanistically, outward TM1 displacement confers structural flexibility that promotes G protein recruitment, whereas inward TM1 retraction facilitates betaarr1 recruitment by stabilizing the intracellular binding pocket through coordinated interactions with TM2, TM7, and helix8. Structural comparisons between the G(i)-, G(z)-, and betaarr1-bound complexes identified a TM1-fusion pocket with significant implications for downstream signaling regulation. Overall, we demonstrate that the conformational and thermodynamic heterogeneity of TM1 allosterically drives the downstream signaling specificity and plasticity of muOR, thereby expanding the understanding of muOR signal transduction mechanisms and providing new avenues for the rational design of analgesics.

The molecular basis of mu-opioid receptor signaling plasticity.,Zhang H, Wang X, Xi K, Shen Q, Xue J, Zhu Y, Zang SK, Yu T, Shen DD, Guo J, Chen LN, Ji SY, Qin J, Dong Y, Zhao M, Yang M, Wu H, Yang G, Zhang Y Cell Res. 2025 Nov 7. doi: 10.1038/s41422-025-01191-8. PMID:41199005<ref>PMID:41199005</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9wsw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Synthetic construct]]
[[Category: Shen Q]]
[[Category: Wang X]]
[[Category: Xi K]]
[[Category: Xue J]]
[[Category: Yang G]]
[[Category: Zhang H]]
[[Category: Zhang Y]]
[[Category: Zhu Y]]