View source for 9wt1


==CryoEM structure of cap module in the contracted AlgoCIS==
<StructureSection load='9wt1' size='340' side='right'caption='[[9wt1]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9wt1]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Algoriphagus_machipongonensis Algoriphagus machipongonensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9WT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9WT1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9wt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9wt1 OCA], [https://pdbe.org/9wt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9wt1 RCSB], [https://www.ebi.ac.uk/pdbsum/9wt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9wt1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A3HTC4_9BACT A3HTC4_9BACT] 
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== Publication Abstract from PubMed ==
Contractile injection systems (CISs) mediate cell-cell interactions and are widespread among bacteria and archaea. These phage tail-like macromolecular machines puncture their target by a tube that is propelled by a contractile sheath. The mechanism underlying CIS firing, which starts with target binding and ends with sheath contraction, remains unclear. Here, we investigate the extracellular CIS from Algoriphagus machipongonensis (AlgoCIS) by a multimodal cryo-electron microscopy approach and structure-guided engineering, which allowed us to arrest AlgoCIS in multiple intermediate states of firing. Together with the post-firing structure, our data suggest a stepwise firing mechanism involving all structural modules: signal propagation starts with the binding of the tail-fibers, followed by opening of the cage, an expansion of the baseplate iris, and resulting in sheath contraction and the release of cap adaptor. Our study will serve as a framework for understanding the firing mechanism of diverse CISs and will facilitate the engineering of CISs for biomedical applications.

Stepwise firing mechanism of an extracellular contractile injection system.,Xu J, Ericson CF, Toenshoff ER, Pilhofer M Nat Commun. 2026 Apr 24. doi: 10.1038/s41467-026-72240-y. PMID:42031783<ref>PMID:42031783</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9wt1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Algoriphagus machipongonensis]]
[[Category: Large Structures]]
[[Category: Ericson CF]]
[[Category: Pilhofer M]]
[[Category: Toenshoff ER]]
[[Category: Xu J]]