View source for 9xxw


==Structure of a putative anti-CRISPR from Streptobacillus moniliformis, ACZ01644==
<StructureSection load='9xxw' size='340' side='right'caption='[[9xxw]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9xxw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptobacillus_moniliformis Streptobacillus moniliformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9XXW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9XXW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9xxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9xxw OCA], [https://pdbe.org/9xxw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9xxw RCSB], [https://www.ebi.ac.uk/pdbsum/9xxw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9xxw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D1AV84_STRM9 D1AV84_STRM9] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The CRISPR-Cas system provides adaptive immunity in bacteria and archaea against invading genetic elements, while anti-CRISPR (Acr) proteins have evolved in phages to counteract this defense. Here, we report the first structural and biochemical characterization of ACZ01644, a protein previously predicted to be an Acr. The crystal structure of ACZ01644 reveals a unique cone-shaped architecture composed of five alpha-helices and five beta-strands forming a compact core, which represents a fold distinct from any known Acr family. Biochemical analyses demonstrated that ACZ01644 assembles as a trimer in solution, suggesting a potential functional relevance of this oligomeric state. However, in vitro assays revealed that ACZ01644 does not inhibit Cas9-mediated DNA cleavage, indicating that its inhibitory activity, if present, may involve other CRISPR subtypes or yet unidentified cofactors. Our findings reveal an unprecedented structural scaffold among putative Acr proteins and provide a foundation for future studies to elucidate its biological role in CRISPR-Cas regulation.

Structural analysis of predicted anti-CRISPR, ACZ01644.,Park SE, Jeong JH, Kim YG, Park HH Biochem Biophys Res Commun. 2026 Jan 25;797:153199. doi: , 10.1016/j.bbrc.2025.153199. Epub 2025 Dec 22. PMID:41453247<ref>PMID:41453247</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9xxw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Streptobacillus moniliformis]]
[[Category: Park HH]]
[[Category: Park SE]]