View source for 9yfz


==KrkA D193C ternary complex==
<StructureSection load='9yfz' size='340' side='right'caption='[[9yfz]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9yfz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9YFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9YFZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1CWR:methyl+3-deoxy-beta-D-manno-oct-2-ulopyranosidonic+acid'>A1CWR</scene>, <scene name='pdbligand=BDR:BETA-D-RIBOFURANOSYL'>BDR</scene>, <scene name='pdbligand=C5P:CYTIDINE-5-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9yfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9yfz OCA], [https://pdbe.org/9yfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9yfz RCSB], [https://www.ebi.ac.uk/pdbsum/9yfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9yfz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A854BIM6_ECOLX A0A854BIM6_ECOLX] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
3-Deoxy-D-manno-octulosonic acid (Kdo) is an eight-carbon monosaccharide that, in Gram-negative bacteria, is an essential structural component of both lipopolysaccharide and the polymeric linker in Group 2 and 3 capsules. Kdo is also an important building block of the variable region of various capsular structures, but the responsible enzymes have, to date, not been investigated. Here, we structurally and functionally characterize the protein KrkA from Escherichia coli capsular serotype K13, showing that it has both ribosyltransferase and Kdo-transferase activity, consistent with this protein being solely responsible for synthesizing the K13 (and the closely related K20 and K23) capsular polysaccharide repeat. We show that the N-terminal module of this protein is the Kdo-transferase. This module's x-ray structure (at 2.7 A resolution) resembles that of two characterized Kdo-transferases: the GT99 module of WbbB, and the GT107 module of KpsC, despite sharing negligible sequence similarity. KrkA is therefore the founding member of a new GT family, GT140. KrkA(GT)(140) is organized into a trimer, where a single residue contributes to a neighboring protomer's acceptor binding site. Using a D193C mutation, we determined the Kdo adduct and ternary complex structures at 2.0 and 2.2 A resolution, respectively. These structures, along with site-directed mutagenesis, confirm the critical nature of the nucleophile Asp193 and the general base Glu102 (contributed by a secondary structure element distinct from that in GT99 and GT107), as well as important adduct and acceptor binding residues. Escherichia coli isolates collectively encode four distinct clusters of related proteins, suggesting polyphyletic origins for this capsular serotype.

The retaining kdo transferase that synthesizes Escherichia coli K13 capsule is deeply divergent from structurally homologous enzymes.,Govind M, Allas MJ, Huang BS, Lowary TL, Kimber MS J Biol Chem. 2026 Jan 13;302(3):111162. doi: 10.1016/j.jbc.2026.111162. PMID:41539565<ref>PMID:41539565</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9yfz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Govind M]]
[[Category: Kimber MS]]