View source for 9yp2


==Structure of HaCV P domain in complex with Nanobody 7==
<StructureSection load='9yp2' size='340' side='right'caption='[[9yp2]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9yp2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hare_calicivirus Hare calicivirus] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9YP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9YP2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9yp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9yp2 OCA], [https://pdbe.org/9yp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9yp2 RCSB], [https://www.ebi.ac.uk/pdbsum/9yp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9yp2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A4D6I9J0_9CALI A0A4D6I9J0_9CALI] Capsid protein VP60 self assembles to form an icosahedral capsid with a T=3 symmetry, about 35 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate VP2 proteins and genomic or subgenomic RNA. Attaches virion to target cells by binding histo-blood group antigens, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm.[ARBA:ARBA00024666]  Displays NTPase activity, but no helicase activity. Induces the formation of convoluted membranes derived from the host ER. These remodeled membranes probably form the viral factories that contain the replication complex. Together with NS2 and NS4, initiates the formation of the replication complex.[ARBA:ARBA00045380]  Probable key protein responsible for the formation of membrane alterations by the virus. Induces the formation of convoluted membranes derived from the host ER. These remodeled membranes probably form the viral factories that contain the replication complex. Together with NS2 and NTPase, initiates the formation of the replication complex.[ARBA:ARBA00046246]  Replicates genomic and antigenomic RNA by recognizing replications specific signals. Also transcribes a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs.[ARBA:ARBA00054946]  Together with NTPase and NS4, initiates the formation of the replication complex. Induces the proliferation of the host smooth ER membranes forming long tubular structures. These remodeled membranes probably form the viral factories that contain the replication complex.[ARBA:ARBA00045264]  Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation. Interacts with host translation initiation complex to allow the translation of viral proteins.[ARBA:ARBA00046180]
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</StructureSection>
[[Category: Hare calicivirus]]
[[Category: Large Structures]]
[[Category: Vicugna pacos]]
[[Category: Hansman G]]
[[Category: Kara D]]
[[Category: Pancera M]]