View source for 9z64


==Structure of the sodium-dependent phosphate importer SLC34A2 S135C, apo==
<StructureSection load='9z64' size='340' side='right'caption='[[9z64]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9z64]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Z64 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Z64 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.11&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9z64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9z64 OCA], [https://pdbe.org/9z64 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9z64 RCSB], [https://www.ebi.ac.uk/pdbsum/9z64 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9z64 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9PTQ8_DANRE Q9PTQ8_DANRE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Dysregulation of inorganic phosphate (Pi) homeostasis contributes to metabolic disease, cancer, pathological calcification, and kidney disease. Systemic phosphate balance is regulated by SLC34 transporters that mediate renal Pi retention (SLC34A1/A3) and intestinal dietary Pi absorption (SLC34A2). SLC34s couple Pi uptake to the symport of sodium (Na (+) ) down its electrochemical gradient. Mutations or altered expression of SLC34 proteins are linked to disorders such as chronic kidney disease (CKD), where hyperphosphatemia is a major complication, and the lung disease pulmonary alveolar microlithiasis (PAM), caused by inactivating SLC34A2 mutations. SLC34A2 is also overexpressed in most ovarian and uterine tumors, making it an attractive target for antibody-drug conjugates. We present cryo-EM structures of SLC34A2 when the transporter is empty, bound to Na (+) ions only, fully loaded with Na (+) ions and Pi, and bound to an inhibitor phosphonoformic acid (PFA), revealing its distinct architecture, substrate and ion binding sites, the role of Na (+) , and multiple transporter states. Pi binds at a highly symmetric, membrane-embedded pocket positioned approximately mid-membrane and is coordinated by its signature QSSS repeat motifs. Na (+) shapes the Pi-binding pocket and drives the transition from the outward-open to occluded state. Integrated with functional analyses, these structures reveal that SLC34 transporters operate through an atypical alternating access cycle defined by coordinated elevator movements of an auxiliary gate domain. This work lays a foundational framework for understanding Pi regulation and opens new avenues for therapeutic strategies targeting disorders linked to phosphate imbalance.

Structural and mechanistic insights into SLC34 phosphate import.,Zhu Q, Almakki O, Diver MM bioRxiv [Preprint]. 2026 May 28:2026.05.25.727688. doi: , 10.64898/2026.05.25.727688. PMID:42244560<ref>PMID:42244560</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9z64" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Danio rerio]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Diver MM]]
[[Category: Zhu Q]]