View source for 9zlk


==Cryo-EM structure of MgtA with bound AMPPCP and magnesium==
<StructureSection load='9zlk' size='340' side='right'caption='[[9zlk]], [[Resolution|resolution]] 2.79&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9zlk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_cremoris Lactococcus cremoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ZLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ZLK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.79&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9zlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9zlk OCA], [https://pdbe.org/9zlk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9zlk RCSB], [https://www.ebi.ac.uk/pdbsum/9zlk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9zlk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A166KLB0_LACLC A0A166KLB0_LACLC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
P-type ATPases represent an evolutionarily conserved superfamily of ion, lipid, and peptide pumps found across all domains of life. Among the substrates transported by P-type ATPases, Mg(2+) is of critical importance in bacterial, fungal, and plant cellular homeostasis. A bacterial P-type ATPase found in Gram-negative bacteria, Mg(2+) transporter A (MgtA), facilitates the transport of Mg(2+) from the periplasm to the cytoplasm under conditions of Mg(2+) starvation. MgtA is a cardiolipin-sensitive integral membrane ion-transporter that scavenges Mg(2+) during bacterial infection and pathogenesis. Here, we determined cryo-EM structures of MgtA capturing three distinct states along the Mg(2+) transport cycle, including a phosphorylated E2-P intermediate (2.6 A resolution), an E1-like conformation stabilized by the peptide regulator MgtR (2.7 A resolution), and an E1-like ATP-bound state (2.8 A resolution). These three conformations reveal the binding of Mg(2+) in the transmembrane domain coordinated in a novel site involving Ser(702) and Asn(706) on M5, Ser(773) and Asp(777) on M7, and Ser(821) and Thr(824) on M8. In the E2-P conformation, the phosphate analog BeF(3) is bound in close proximity to the catalytic aspartate, Asp(361), suggesting that it represents a covalent aspartylphosphate intermediate. In the presence of AMPPCP, Mg(2+) remains bound in the transmembrane domain and the ATP analog is bound in a catalytically competent conformation. Overall, the structures reveal distinct steps in the transport cycle of MgtA compared to other P-type ATPases, as well as lipid binding sites that fill gaps in our understanding of transport regulation.

Distinct transport cycle and lipid regulation of a Mg(2+)-transporting P-type ATPase, MgtA.,Khan MB, Primeau JO, Chaudhuri-Basu P, Bergdoll L, Renault L, Morth JP, Lemieux MJ, Young HS Res Sq [Preprint]. 2026 May 4:rs.3.rs-9173029. doi: 10.21203/rs.3.rs-9173029/v1. PMID:42147146<ref>PMID:42147146</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9zlk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lactococcus cremoris]]
[[Category: Large Structures]]
[[Category: Basu PC]]
[[Category: Khan MB]]
[[Category: Lemieux MJ]]
[[Category: Morth JP]]
[[Category: Primeau JO]]
[[Category: Young HS]]