View source for Aspirin effects on COX aka PGHS

===ASPIRIN'S ANTI-INFLAMMATORY EFFECTS UPON PROSTAGLANDIN H2 SYNTHASE (CYCLOOXYGENASE)===

<StructureSection load='1pth' size='340' side='right' caption='Caption for this structure'cyclooxygenase I scene=''>

[[Aspirin]], also known as acetyl salicylic acid or ASA, has been used as an antipyretic drug (reduces fever) and as an anti-inflammatory agent (reduces swelling and redness due to inflammation) from ancient times. Chewing on willow bark was a headache and toothache remedy for American Indians<ref>http://www.bellaonline.com/articles/art8190.asp</ref>, and Dioscorides, the first century Greek physician, recommended it as a poultice for treating inflammation in his De Materia Medica Libri .<ref name="Mayer"> Quinque Mayer R, Mayer M. Mayer R, Mayer M. Biological salicyltherapy with cortex salicus [Weidenrinde]. Pharmazie 1949;4:77-81.</ref>.  Aspirin is in the family of drugs known as NSAIDs, which stands for '''N'''on'''S'''teroidal '''A'''nti'''I'''nflammatory '''D'''rugs.
 
While the use of aspirin goes back to ancient times, the mechanism of how aspirin relieves pain is relatively modern.  In 1971, John Vane discovered that aspirin works by inhibiting the synthesis of prostaglandins <ref>PMID:5284360</ref>, and he recieved a Nobel Prize for this work in 1982 <ref>http://nobelprize.org/nobel_prizes/medicine/laureates/1982/vane-lecture.html</ref>. In 1975 Roth, Standford, and Majerus identified prostaglandin H2 synthase, also referred to as cyclooxygenase (COX), as the enzyme that aspirin inhibits<ref>PMID 810797</ref>.  

PGHS contains two identical polypeptide chains, shown in blue and green in the box at the right.  The enzyme catalyzes two reactions:  first, the <scene name='Aspirin_effects_on_COX_aka_PGHS/Heme_1/5'>heme</scene> reacts with the oxygen to create a peroxide at <scene name='37/377115/Aa_tyr_heme/1'>tyrosine 385</scene>; in the second reaction, the peroxide is added to the double bond in arachadonic acid.  
Aspirin works by transferring the acetyl group (CH3COO-) of aspirin to the OH of the <scene name='37/377115/Ser_530_with_label/1'>Ser 530</scene>.  When this residue is acetylated, arachidonic acid can no longer be bound because there is no longer room for it to fit in the enzyme.  Since the acetylation cannot be undone, this is a "suicide" inhibitor--the inhibition cannot be undone, and the enzyme is dead.

</StructureSection>

==About this Structure==
1PTH is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries]. The May 2001 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb17_1.html Molecule of the Month]by David S. Goodsell features ''Cyclooxygenase''.  [http://dx.doi.org/10.2210/rcsb_pdb/mom_2001_510.2210/rcsb_pdb/mom_2001_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PTH OCA].

==Other Tutorials==
* [https://moodle.tu-dortmund.de/pluginfile.php/631894/mod_resource/content/8/webseiteDMUV-JSmol/AB-T4-Frameset-Digital-Media-Aspirin-inhibition-of-COX-1-vers4.htm The effect of ASPIRIN: the irreversible inhibition of Cyclooxygenase-1] is a more detailed tutorial by Andreas Brink, Technische Universität Dortmund. Also available in German/Auch in deutscher Sprache erhältlich.

==References==
<references/>
[[Category: Cyclooxygenase]]
[[Category: Prostaglandin-endoperoxide synthase]]
[[Category: Dioxygenase]]
[[Category: Peroxidase]]