View source for V-ATPase

==Introduction==
Vacuolar (H+)-ATPases (V-ATPases)<ref>PMID:17912264</ref> are mainly found in vacuoles of eukaryotic cells where they catalyze the hydrolysis of [[ATP]] in order to transport solutes. V-ATPases are structurally and mechanically related to F- and A-ATPases.<ref>PMID:15473999</ref>

==V-ATPase components==
The structure of the whole V-ATPase complex can be divided in two domains. The V1 domain, which consist of eight different sub-units (A-H) and is responsible for the hydrolysis of ATP, and the intermembrane V0 domain consisting of six different sub-units (a, d, e, c, c' and c") and which acts as a proton translocator from the cytoplasm to the lumen.<ref>PMID:20450191</ref>

ATP hydrolysis occurs at catalytic sites located at the interface of the A and B subunits.

===V1-domain===
<StructureSection load='3a5c' size='250' side='right' caption='V1-domain of V-ATPase of Thermus Thermophilus (PDB entry [[3j0j]])' scene='V-ATPase/V1-part/1'>
The <scene name='V-ATPase/V1-part/1'>V1-domain</scene> consist of the A3B3 complex where the hydrolysis of ATP occurs (<scene name='V-ATPase/V1-part/3'>A is blue, B is red</scene>), subunit C (<scene name='V-ATPase/V1-part/8'>green</scene>), the D-subunit which is the central stalk (<scene name='V-ATPase/V1-part/2'>purple</scene>), subunit E (<scene name='V-ATPase/V1-part/6'>orange</scene>), subunit F (<scene name='V-ATPase/V1-part/4'>yellow</scene>)and subunit G (<scene name='V-ATPase/V1-part/7'>dark green</scene>).
</StructureSection>

===V0-domain===

==Mechanism of rotation==

==3D V-ATPase structures==

See [[ATPase]]

===EMDB===
[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/5335_summary.html 5335]: 9.7&Aring; resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1888_summary.html 1888]: 16&Aring; resolution map of Thermus Thermophilus V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1640_summary.html 1640]: 25&Aring; resolution map of Saccharomyces cerevisiae V-ATPase.

[http://www.ebi.ac.uk/pdbe-srv/emsearch/atlas/1590_summary.html 1590]: 17&Aring; resolution map of Manduca sexta V-ATPase.

==References==

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