View source for WspR

<StructureSection load='3bre' size='350' side='right' scene='User:Tilman_Schirmer/Sandbox_204/Protomer/3' caption='Two-component response regulator (PDB code [[3bre]])'>
[[C-di-GMP signaling|Back to C-di-GMP signaling]]


== Overview ==

<scene name='User:Tilman_Schirmer/Sandbox_204/Protomer/3'>WspR </scene> from ''Pseudomonas aeruginosa'' is a response regulator with an catalytic, diguanylate cyclase, output domain. It is composed of a canonical CheY-like response regulator receiver domain (<scene name='User:Tilman_Schirmer/Sandbox_204/Rec/2'>Rec</scene>) and a C-terminal <scene name='User:Tilman_Schirmer/Sandbox_204/Ggdef/2'>catalytic GGDEF domain</scene> domain that confers the catalytic activity with all canonical <scene name='User:Tilman_Schirmer/Sandbox_204/Substrate_binding_site/2'>active site residues</scene> present.


<br>Although not modified (i.e. phosphorylated) at the active Asp (<scene name='User:Tilman_Schirmer/Sandbox_204/Rec/4'>Asp70</scene>), the Rec domains mediate formation of <scene name='User:Tilman_Schirmer/Sandbox_204/Dimer/1'>dimeric </scene> WspR. Two dimers, in turn, are associated by head-to-head contact to a <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/1'>tetramer</scene> of approximate 222 (D2) symmetry. 

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== Allosteric product binding site ==

<scene name='User:Tilman_Schirmer/Sandbox_204/Ip/3'>WspR</scene> ([[3bre]])

There are two allosteric sites (<scene name='User:Tilman_Schirmer/Sandbox_204/Ip/3'>primary inhibition site, Ip,</scene> and  <scene name='User:Tilman_Schirmer/Sandbox_204/Is/3'>secondary inhibition site, Is</scene>) that become cross-linked by (c-di-GMP)<sub>2</sub> dimers in the <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/5'>tetrameric</scene> molecule. For  a close-up click <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/4'>here</scene> (<scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/6'>Ip-site</scene>, <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/8'>Is-site</scene>, <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/9'>(c-di-GMP dimer)2</scene>). Note that there are four (c-di-GMP)<sub>2</sub> dimers per WspR tetramer.

</StructureSection>
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==3D structures of response regulator==

[[Response regulator]]

==References==
WspR structure [[3bre]]:
<ref group="xtra">PMID:18366254</ref>
<references group="xtra"/>