OCA at 06:54, 15 July 2026

2026-07-15T06:54:39Z

← Older revision		Revision as of 06:54, 15 July 2026
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	~~'''Unreleased structure'''~~

	~~The entry~~ 10fa is ~~ON HOLD until Paper Publication~~		==E. coli tRNA guanine transgylcosylase==
			<StructureSection load='10fa' size='340' side='right'caption='[[10fa]], [[Resolution\|resolution]] 3.52Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[10fa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=10FA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=10FA FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.52Å</td></tr>
	[[Category: ~~Unreleased~~ Structures]]		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=10fa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=10fa OCA], [https://pdbe.org/10fa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=10fa RCSB], [https://www.ebi.ac.uk/pdbsum/10fa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=10fa ProSAT]</span></td></tr>
			</table>
			== Function ==
			[https://www.uniprot.org/uniprot/TGT_ECOLI TGT_ECOLI] Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).[HAMAP-Rule:MF_00168]<ref>PMID:11714265</ref> <ref>PMID:12909636</ref>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Escherichia coli]]
			[[Category: Large Structures]]
			[[Category: Devaraj N]]
			[[Category: Harjung A]]

OCA: Protected "10fa": Seeded page ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))

2026-02-10T07:09:03Z

Protected "10fa": Seeded page ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))

← Older revision	Revision as of 07:09, 10 February 2026
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OCA: Created page with "'''Unreleased structure''' The entry 10fa is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures"

2026-02-10T07:09:02Z

Created page with "'''Unreleased structure''' The entry 10fa is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures"

New page

'''Unreleased structure'''

The entry 10fa is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

10fa - Revision history

OCA at 06:54, 15 July 2026

OCA: Protected "10fa": Seeded page ([Edit=Allow only administrators] (indefinite) [Move=Allow only administrators] (indefinite))

OCA: Created page with "'''Unreleased structure''' The entry 10fa is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures"