1a67 - Revision history

OCA at 04:22, 17 October 2024

2024-10-17T04:22:23Z

← Older revision		Revision as of 04:22, 17 October 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A67 FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 16 models</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [https://pdbe.org/1a67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [https://www.ebi.ac.uk/pdbsum/1a67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a67 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [https://pdbe.org/1a67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [https://www.ebi.ac.uk/pdbsum/1a67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a67 ProSAT]</span></td></tr>
	</table>		</table>
Line 14:		Line 14:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/1a67_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/1a67_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a67 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a67 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The solution structures of the phosphorylated form of native chicken cystatin and the recombinant variant AEF-S1M-M29I-M89L were determined by 2D, 3D and 4D-NMR. The structures turn out to be very similar, despite the substitutions and the phosphorylation of the wild-type. Their dominant feature is a five-stranded beta-sheet, which is wrapped around a five-turn alpha-helix, as shown by X-ray crystallographic studies of wild-type chicken cystatin. However, the NMR analysis shows that the second helix observed in the crystal is not present in solution. The phosphorylation occurs at S80, which is located in a flexible region. For this reason, very few effects on the structure are observed. Comparison of structures of the unphosphorylated variant and the wild-type shows small effects on H84 which is located in the supposed recognition site of the serine kinase. This recognition site appears to be well structured as a large loop-containing bulge of the beta-sheet. The N termini of both mutants, which contribute to a large extent to the binding to the proteinase, are very flexible. A loop structure involving the residues L7 to A10 as found in related inhibitors, such as in the kininogen domains 2 and 3, is not sufficiently populated to be observed.

			The structures of native phosphorylated chicken cystatin and of a recombinant unphosphorylated variant in solution.,Dieckmann T, Mitschang L, Hofmann M, Kos J, Turk V, Auerswald EA, Jaenicke R, Oschkinat H J Mol Biol. 1993 Dec 20;234(4):1048-59. PMID:8263912<ref>PMID:8263912</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 1a67" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:21, 13 March 2024

2024-03-13T15:21:36Z

← Older revision		Revision as of 15:21, 13 March 2024
Line 1:		Line 1:

	==CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES==		==CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES==
	<StructureSection load='1a67' size='340' side='right'caption='[[1a67~~]], [[NMR_Ensembles_of_Models \| 16 NMR models~~]]' scene=''>		<StructureSection load='1a67' size='340' side='right'caption='[[1a67]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A67 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [https://pdbe.org/1a67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [https://www.ebi.ac.uk/pdbsum/1a67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a67 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [https://pdbe.org/1a67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [https://www.ebi.ac.uk/pdbsum/1a67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a67 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/CYT_CHICK CYT_CHICK]] This protein binds tightly to and inhibits a variety of thiol proteases including ficin, papain, and cathepsins B, C, H, and L. Although isolated from egg white, it is also present in serum.		[https://www.uniprot.org/uniprot/CYT_CHICK CYT_CHICK] This protein binds tightly to and inhibits a variety of thiol proteases including ficin, papain, and cathepsins B, C, H, and L. Although isolated from egg white, it is also present in serum.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a67 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a67 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The solution structures of the phosphorylated form of native chicken cystatin and the recombinant variant AEF-S1M-M29I-M89L were determined by 2D, 3D and 4D-NMR. The structures turn out to be very similar, despite the substitutions and the phosphorylation of the wild-type. Their dominant feature is a five-stranded beta-sheet, which is wrapped around a five-turn alpha-helix, as shown by X-ray crystallographic studies of wild-type chicken cystatin. However, the NMR analysis shows that the second helix observed in the crystal is not present in solution. The phosphorylation occurs at S80, which is located in a flexible region. For this reason, very few effects on the structure are observed. Comparison of structures of the unphosphorylated variant and the wild-type shows small effects on H84 which is located in the supposed recognition site of the serine kinase. This recognition site appears to be well structured as a large loop-containing bulge of the beta-sheet. The N termini of both mutants, which contribute to a large extent to the binding to the proteinase, are very flexible. A loop structure involving the residues L7 to A10 as found in related inhibitors, such as in the kininogen domains 2 and 3, is not sufficiently populated to be observed.

	The structures of native phosphorylated chicken cystatin and of a recombinant unphosphorylated variant in solution.,Dieckmann T, Mitschang L, Hofmann M, Kos J, Turk V, Auerswald EA, Jaenicke R, Oschkinat H J Mol Biol. 1993 Dec 20;234(4):1048-59. PMID:8263912<ref>PMID:8263912</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1a67" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Auerswald~~, E A~~]]		[[Category: Auerswald EA]]
	[[Category: Dieckmann, T]]		[[Category: Dieckmann T]]
	[[Category: Hofmann, M]]		[[Category: Hofmann M]]
	[[Category: Jaenicke, R]]		[[Category: Jaenicke R]]
	[[Category: Kos, J]]		[[Category: Kos J]]
	[[Category: Mitschang, L]]		[[Category: Mitschang L]]
	[[Category: Oschkinat, H]]		[[Category: Oschkinat H]]
	[[Category: Turk, V]]		[[Category: Turk V]]
	~~[[Category: Kininogen]]~~
	~~[[Category: Proteinase inhibitor]]~~
	~~[[Category: Steffin]]~~
	~~[[Category: Thiol proteinase~~]]

OCA at 09:42, 26 May 2021

2021-05-26T09:42:39Z

← Older revision		Revision as of 09:42, 26 May 2021
Line 3:		Line 3:
	<StructureSection load='1a67' size='340' side='right'caption='[[1a67]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='1a67' size='340' side='right'caption='[[1a67]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1A67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A67 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [~~http~~://pdbe.org/1a67 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1a67 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1a67 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [https://pdbe.org/1a67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [https://www.ebi.ac.uk/pdbsum/1a67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a67 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/CYT_CHICK CYT_CHICK]] This protein binds tightly to and inhibits a variety of thiol proteases including ficin, papain, and cathepsins B, C, H, and L. Although isolated from egg white, it is also present in serum.		[[https://www.uniprot.org/uniprot/CYT_CHICK CYT_CHICK]] This protein binds tightly to and inhibits a variety of thiol proteases including ficin, papain, and cathepsins B, C, H, and L. Although isolated from egg white, it is also present in serum.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:25, 21 August 2019

2019-08-21T07:25:25Z

← Older revision		Revision as of 07:25, 21 August 2019
Line 1:		Line 1:

	==CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES==		==CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES==
	<StructureSection load='1a67' size='340' side='right' caption='[[1a67]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='1a67' size='340' side='right'caption='[[1a67]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A67 FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/1a67_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/1a67_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
			[[Category: Large Structures]]
	[[Category: Auerswald, E A]]		[[Category: Auerswald, E A]]
	[[Category: Dieckmann, T]]		[[Category: Dieckmann, T]]

OCA at 10:48, 15 November 2017

2017-11-15T10:48:19Z

← Older revision		Revision as of 10:48, 15 November 2017
Line 1:		Line 1:

	==CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES==		==CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES==
	<StructureSection load='1a67' size='340' side='right' caption='[[1a67]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='1a67' size='340' side='right' caption='[[1a67]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chick Chick~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A67 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [http://pdbe.org/1a67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [http://www.ebi.ac.uk/pdbsum/1a67 PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [http://pdbe.org/1a67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [http://www.ebi.ac.uk/pdbsum/1a67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1a67 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Chick~~]]		[[Category: Gallus gallus]]
	[[Category: Auerswald, E A]]		[[Category: Auerswald, E A]]
	[[Category: Dieckmann, T]]		[[Category: Dieckmann, T]]

OCA at 02:38, 8 February 2016

2016-02-08T02:38:32Z

← Older revision		Revision as of 02:38, 8 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a67 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:05, 9 September 2015

2015-09-09T16:05:34Z

← Older revision		Revision as of 16:05, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1a67' size='340' side='right' caption='[[1a67]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='1a67' size='340' side='right' caption='[[1a67]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A67 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [http://www.ebi.ac.uk/pdbsum/1a67 PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [http://pdbe.org/1a67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [http://www.ebi.ac.uk/pdbsum/1a67 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1a67" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Gallus gallus~~]]		[[Category: Chick]]
	[[Category: Auerswald, E A]]		[[Category: Auerswald, E A]]
	[[Category: Dieckmann, T]]		[[Category: Dieckmann, T]]

OCA at 19:57, 24 December 2014

2014-12-24T19:57:42Z

← Older revision		Revision as of 19:57, 24 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [http://www.ebi.ac.uk/pdbsum/1a67 PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [http://www.ebi.ac.uk/pdbsum/1a67 PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CYT_CHICK CYT_CHICK]] This protein binds tightly to and inhibits a variety of thiol proteases including ficin, papain, and cathepsins B, C, H, and L. Although isolated from egg white, it is also present in serum.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:52, 22 December 2014

2014-12-22T08:52:14Z

← Older revision		Revision as of 08:52, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A67 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A67 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [http://www.ebi.ac.uk/pdbsum/1a67 PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [http://www.ebi.ac.uk/pdbsum/1a67 PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Auerswald, E A.]]		[[Category: Auerswald, E A]]
	[[Category: Dieckmann, T.]]		[[Category: Dieckmann, T]]
	[[Category: Hofmann, M.]]		[[Category: Hofmann, M]]
	[[Category: Jaenicke, R.]]		[[Category: Jaenicke, R]]
	[[Category: Kos, J.]]		[[Category: Kos, J]]
	[[Category: Mitschang, L.]]		[[Category: Mitschang, L]]
	[[Category: Oschkinat, H.]]		[[Category: Oschkinat, H]]
	[[Category: Turk, V.]]		[[Category: Turk, V]]
	[[Category: Kininogen]]		[[Category: Kininogen]]
	[[Category: Proteinase inhibitor]]		[[Category: Proteinase inhibitor]]
	[[Category: Steffin]]		[[Category: Steffin]]
	[[Category: Thiol proteinase]]		[[Category: Thiol proteinase]]

OCA at 08:37, 23 July 2014

2014-07-23T08:37:31Z

← Older revision		Revision as of 08:37, 23 July 2014
Line 1:		Line 1:
	[[Image:~~1a67~~.~~png~~\|~~left~~\|~~200px~~]]		==CHICKEN EGG WHITE CYSTATIN WILDTYPE, NMR, 16 STRUCTURES==
			<StructureSection load='1a67' size='340' side='right' caption='[[1a67]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1a67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A67 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a67 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1a67 RCSB], [http://www.ebi.ac.uk/pdbsum/1a67 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/1a67_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The solution structures of the phosphorylated form of native chicken cystatin and the recombinant variant AEF-S1M-M29I-M89L were determined by 2D, 3D and 4D-NMR. The structures turn out to be very similar, despite the substitutions and the phosphorylation of the wild-type. Their dominant feature is a five-stranded beta-sheet, which is wrapped around a five-turn alpha-helix, as shown by X-ray crystallographic studies of wild-type chicken cystatin. However, the NMR analysis shows that the second helix observed in the crystal is not present in solution. The phosphorylation occurs at S80, which is located in a flexible region. For this reason, very few effects on the structure are observed. Comparison of structures of the unphosphorylated variant and the wild-type shows small effects on H84 which is located in the supposed recognition site of the serine kinase. This recognition site appears to be well structured as a large loop-containing bulge of the beta-sheet. The N termini of both mutants, which contribute to a large extent to the binding to the proteinase, are very flexible. A loop structure involving the residues L7 to A10 as found in related inhibitors, such as in the kininogen domains 2 and 3, is not sufficiently populated to be observed.

	~~{{STRUCTURE_1a67\| PDB=1a67 \| SCENE= }}~~		The structures of native phosphorylated chicken cystatin and of a recombinant unphosphorylated variant in solution.,Dieckmann T, Mitschang L, Hofmann M, Kos J, Turk V, Auerswald EA, Jaenicke R, Oschkinat H J Mol Biol. 1993 Dec 20;234(4):1048-59. PMID:8263912<ref>PMID:8263912</ref>

	~~===CHICKEN EGG WHITE CYSTATIN WILDTYPE~~, ~~NMR, 16 STRUCTURES===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_8263912}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[1a67]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Gallus_gallus Gallus gallus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1A67 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:008263912<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Auerswald, E A.]]		[[Category: Auerswald, E A.]]