1ap1 - Revision history

OCA at 11:33, 22 November 2023

2023-11-22T11:33:13Z

← Older revision		Revision as of 11:33, 22 November 2023
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	==THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE==		==THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE==
	<StructureSection load='1ap1' size='340' side='right'caption='[[1ap1~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>		<StructureSection load='1ap1' size='340' side='right'caption='[[1ap1]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>
	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [https://pdbe.org/1ap1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ap1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ap1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [https://pdbe.org/1ap1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ap1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ap1 ProSAT]</span></td></tr>
	</table>		</table>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Setayesh~~, F R~~]]		[[Category: Setayesh FR]]
	[[Category: Stone~~, M P]]~~		[[Category: Stone MP]]
	~~[[Category: Codon 12 sequence]]~~
	~~[[Category: Deoxyribonucleic acid]]~~
	~~[[Category: Dna]]~~
	~~[[Category: Dna duplex]]~~
	~~[[Category: Human n-ras gene]]~~
	~~[[Category: Minor groove adduct]]~~
	~~[[Category: S-styrene oxide~~]]

OCA at 10:22, 17 February 2021

2021-02-17T10:22:33Z

← Older revision		Revision as of 10:22, 17 February 2021
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	<StructureSection load='1ap1' size='340' side='right'caption='[[1ap1]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1ap1' size='340' side='right'caption='[[1ap1]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [~~http~~://pdbe.org/1ap1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ap1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ap1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [https://pdbe.org/1ap1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ap1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ap1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:45, 10 May 2019

2019-05-10T14:45:14Z

← Older revision		Revision as of 14:45, 10 May 2019
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	==THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE==		==THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE==
	<StructureSection load='1ap1' size='340' side='right' caption='[[1ap1]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1ap1' size='340' side='right'caption='[[1ap1]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Setayesh, F R]]		[[Category: Setayesh, F R]]
	[[Category: Stone, M P]]		[[Category: Stone, M P]]

OCA at 09:12, 9 August 2017

2017-08-09T09:12:53Z

← Older revision		Revision as of 09:12, 9 August 2017
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	==THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE==		==THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE==
	<StructureSection load='1ap1' size='340' side='right' caption='[[1ap1]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1ap1' size='340' side='right' caption='[[1ap1]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
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	<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [http://pdbe.org/1ap1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ap1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [http://pdbe.org/1ap1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ap1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ap1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:40, 11 September 2015

2015-09-11T02:40:30Z

← Older revision		Revision as of 02:40, 11 September 2015
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	<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ap1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [http://pdbe.org/1ap1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ap1 PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1ap1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:31, 22 December 2014

2014-12-22T06:31:40Z

← Older revision		Revision as of 06:31, 22 December 2014
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ap1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ap1 PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Setayesh, F R.]]		[[Category: Setayesh, F R]]
	[[Category: Stone, M P.]]		[[Category: Stone, M P]]
	[[Category: Codon 12 sequence]]		[[Category: Codon 12 sequence]]
	[[Category: Deoxyribonucleic acid]]		[[Category: Deoxyribonucleic acid]]

OCA at 05:35, 8 June 2014

2014-06-08T05:35:25Z

← Older revision		Revision as of 05:35, 8 June 2014
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	[[~~Image~~:1ap1.~~png\|left\|200px~~]]		==THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE==
			<StructureSection load='1ap1' size='340' side='right' caption='[[1ap1]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP1 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSS:2-DEOXY-N2-(S)STYRENE+OXIDE+GUANOSINE+MONOPHOSPHATE'>GSS</scene></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ap1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ap1 RCSB], [http://www.ebi.ac.uk/pdbsum/1ap1 PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The R- and S-alpha-(N2-guanyl)styrene oxide (SO) adducts at X5 in d(G1G2C3A4X5G6T7G8G9T10G11).d(C12A13C14C15A16C17C18T19G20C21C22 ), encompassing codon 12 of the human N-ras protooncogene (underlined), were examined using 1H NMR spectroscopy. These were the R(12,1) and S(12,1) adducts, indicating the location of the R or S adduct at the first position of codon 12. These differed from the R- and S(12, 2)-alpha-SO adducts [Zegar, I. S., Setayesh, F. R., DeCorte, B. L., Harris, C. M., Harris, T. M., and Stone, M. P. (1996) Biochemistry 35, 4334-4348] in that the base pair 5' to the lesion was changed from G.C to A.T, while the base pair 3' to the lesion was changed from T.A to G.C. Comparison of the R- and S(12,1) adducts with the R- and S(12,2) adducts allowed the effects of flanking bases on the conformations of the alpha-SO adducts to be examined. This change in flanking base affected the R-SO lesion. The R(12,1) adduct structure was disordered at the adduct site, and a refined structure could not be obtained. NOE and chemical shift data suggested that the styrenyl moiety was oriented in the minor groove and in the 3'-direction from the site of adduction. In contrast, this change in flanking base did not affect the S-SO lesion. The S(12,1) adduct yielded a refined structure, with the styrenyl moiety edgewise in the minor groove and oriented in the 5'-direction relative to the site of adduction. A total of 232 interproton distances, including 13 styrene-DNA distances, were obtained. A total of 12 NOE-restrained molecular dynamics calculations converged with pairwise root-mean-square deviation of 1.10 A. The sixth-root residual index between calculated and experimental NOE intensities was 8.0 x 10(-)2 A. The styrene aromatic protons appeared as three resonances, suggesting rapid rotation. The possibility of a hydrogen bond between the styrene hydroxyl and C18 O2 in the S(12,1) adduct could not be confirmed. This work illustrates the dual roles of stereochemistry and sequence in modulating the properties of guanine N2 alpha-SO adducts.

	~~{{STRUCTURE_1ap1\| PDB=1ap1 \| SCENE= }}~~		Styrene oxide adducts in an oligodeoxynucleotide containing the human N-ras codon 12: minor groove structures of the R(12,1)- and S(12,1)-alpha-(N2-guanyl) stereoisomers determined by 1H nuclear magnetic resonance.,Setayesh FR, DeCorte BL, Horton P, Harris CM, Harris TM, Stone MP Chem Res Toxicol. 1998 Jul;11(7):766-77. PMID:9671539<ref>PMID:9671539</ref>

	===THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, ~~MINIMIZED AVERAGE STRUCTURE===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_9671539}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[1ap1]] is~~ a ~~2 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=1AP1 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:009671539<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Setayesh, F R.]]		[[Category: Setayesh, F R.]]
	[[Category: Stone, M P.]]		[[Category: Stone, M P.]]

OCA: Protected "1ap1" [edit=sysop:move=sysop]

2012-08-14T18:41:21Z

Protected "1ap1" [edit=sysop:move=sysop]

← Older revision	Revision as of 18:41, 14 August 2012
(No difference)

OCA at 18:41, 14 August 2012

2012-08-14T18:41:19Z

← Older revision		Revision as of 18:41, 14 August 2012
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	~~{{Seed}}~~
	[[Image:1ap1.png\|left\|200px]]		[[Image:1ap1.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_1ap1", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_1ap1\| PDB=1ap1 \| SCENE= }}		{{STRUCTURE_1ap1\| PDB=1ap1 \| SCENE= }}

	===THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE===		===THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE===


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	{{ABSTRACT_PUBMED_9671539}}		{{ABSTRACT_PUBMED_9671539}}

	==About this Structure==		==About this Structure==
	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA].		[[1ap1]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA].

	==Reference==		==Reference==
	Styrene oxide adducts in an oligodeoxynucleotide containing the human N-ras codon 12: minor groove structures of the R(12,1)- and S(12,1)-alpha-(N2-guanyl) stereoisomers determined by 1H nuclear magnetic resonance., Setayesh FR, DeCorte BL, Horton P, Harris CM, Harris TM, Stone MP, Chem Res Toxicol. 1998 Jul;11(7):766-77. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~9671539 9671539]~~		<ref group="xtra">PMID:009671539</ref><references group="xtra"/>
	[[Category: Setayesh, F R.]]		[[Category: Setayesh, F R.]]
	[[Category: Stone, M P.]]		[[Category: Stone, M P.]]
	[[Category: Codon 12 sequence]]		[[Category: Codon 12 sequence]]
	[[Category: Deoxyribonucleic acid]]		[[Category: Deoxyribonucleic acid]]
			[[Category: Dna]]
	[[Category: Dna duplex]]		[[Category: Dna duplex]]
	[[Category: Human n-ras gene]]		[[Category: Human n-ras gene]]
	[[Category: Minor groove adduct]]		[[Category: Minor groove adduct]]
	[[Category: S-styrene oxide]]		[[Category: S-styrene oxide]]

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2008-06-30T14:19:54Z

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	{{STRUCTURE_1ap1\| PDB=1ap1 \| SCENE= }}		{{STRUCTURE_1ap1\| PDB=1ap1 \| SCENE= }}

	~~'''~~THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE~~'''~~		===THE SOLUTION NMR STRUCTURE OF AN S-STYRENE OXIDE ADDUCT AT THE N2 POSITION OF GUANINE OF AN 11 BASE-PAIR OLIGONUCLEOTIDE SEQUENCE CODING FOR AMINO ACIDS 11-13 OF THE PRODUCT OF THE N-RAS PROTOONCOGENE, MINIMIZED AVERAGE STRUCTURE===


	~~==Overview==~~		<!--
	The R- and S-alpha-(N2-guanyl)styrene oxide (SO) adducts at X5 in d(G1G2C3A4X5G6T7G8G9T10G11).d(C12A13C14C15A16C17C18T19G20C21C22 ), encompassing codon 12 of the human N-ras protooncogene (underlined), were examined using 1H NMR spectroscopy. These were the R(12,1) and S(12,1) adducts, indicating the location of the R or S adduct at the first position of codon 12. These differed from the R- and S(12, 2)-alpha-SO adducts [Zegar, I. S., Setayesh, F. R., DeCorte, B. L., ~~Harris~~, ~~C. M., Harris, T. M., and Stone, M. P. (1996) Biochemistry 35, 4334-4348] in that~~ the ~~base pair 5'~~ to the ~~lesion was changed from G.C to A.T, while the base pair 3' to the lesion was changed from T.A to G.C. Comparison of the R- and S~~(~~12,1) adducts with the R- and S(12,2) adducts allowed the effects of flanking bases~~ on ~~the conformations of the alpha-SO adducts to be examined. This change in flanking base affected the R-SO lesion. The R(12,1) adduct structure was disordered~~ at ~~the adduct site, and a refined structure could not be obtained~~. ~~NOE and chemical shift data suggested that the styrenyl moiety was oriented in the minor groove and in the 3'-direction from the site of adduction~~. ~~In contrast, this change in flanking base did not affect the S-SO lesion. The S(12,1~~) ~~adduct yielded a refined structure~~, ~~with the styrenyl moiety edgewise in the minor groove and oriented in the 5'-direction relative to~~ the ~~site of adduction~~. ~~A total of 232 interproton distances, including 13 styrene~~-~~DNA distances, were obtained. A total of 12 NOE~~-restrained molecular dynamics calculations converged with pairwise root-mean-square deviation of 1.10 A. The sixth-root residual index between calculated and experimental NOE intensities was 8.0 x 10(-)2 A. The styrene aromatic protons appeared as three resonances, suggesting rapid rotation. The possibility of a hydrogen bond between the styrene hydroxyl and C18 O2 in the S(12,1) adduct could not be confirmed. This work illustrates the dual roles of stereochemistry and sequence in modulating the properties of guanine N2 alpha-SO adducts.		The line below this paragraph, {{ABSTRACT_PUBMED_9671539}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 9671539 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_9671539}}

	==About this Structure==		==About this Structure==
	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA].		Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP1 OCA].

	==Reference==		==Reference==
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	[[Category: Minor groove adduct]]		[[Category: Minor groove adduct]]
	[[Category: S-styrene oxide]]		[[Category: S-styrene oxide]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May 2 10~~:32:25 2008''
			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:19:53 2008''