1cgi - Revision history

OCA at 06:29, 30 October 2024

2024-10-30T06:29:27Z

← Older revision		Revision as of 06:29, 30 October 2024
Line 14:		Line 14:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/1cgi_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/1cgi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>

OCA at 05:26, 5 June 2024

2024-06-05T05:26:28Z

← Older revision		Revision as of 05:26, 5 June 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cgi ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cgi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Variants of the human pancreatic secretory trypsin inhibitor (PSTI) have been created during a protein design project to generate a high-affinity inhibitor with respect to some serine proteases other than trypsin. Two modified versions of human PSTI with high affinity for chymotrypsin were crystallized as a complex with chymotrypsinogen. Both crystallize isomorphously in space group P4(1)2(1)2 with lattice constants a = 84.4 A, c = 86.7 A and diffract to 2.3 A resolution. The structure was solved by molecular replacement. The final R-value after refinement with 8.0 to 2.3 A resolution data was 19.5% for both complexes after inclusion of about 50 bound water molecules. The overall three-dimensional structure of PSTI is similar to the structure of porcine PSTI in the trypsinogen complex (1TGS). Small differences in the relative orientation of the binding loop and the core of the inhibitors indicate flexible adaptation to the proteases. The chymotrypsinogen part of the complex is similar to chymotrypsin. After refolding induced by binding of the inhibitor the root-mean-square difference of the active site residues A186 to A195 and A217 to A222 compared to chymotrypsin was 0.26 A.

			Three-dimensional structure of the complexes between bovine chymotrypsinogen A and two recombinant variants of human pancreatic secretory trypsin inhibitor (Kazal-type).,Hecht HJ, Szardenings M, Collins J, Schomburg D J Mol Biol. 1991 Aug 5;220(3):711-22. PMID:1870127<ref>PMID:1870127</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 1cgi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Chymotrypsin 3D structures\|Chymotrypsin 3D structures]]		*[[Chymotrypsin 3D structures\|Chymotrypsin 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:40, 13 March 2024

2024-03-13T15:40:01Z

← Older revision		Revision as of 15:40, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='1cgi' size='340' side='right'caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cgi' size='340' side='right'caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~] and [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CGI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CGI FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgi OCA], [https://pdbe.org/1cgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cgi RCSB], [https://www.ebi.ac.uk/pdbsum/1cgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cgi ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgi OCA], [https://pdbe.org/1cgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cgi RCSB], [https://www.ebi.ac.uk/pdbsum/1cgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cgi ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/CTRA_BOVIN CTRA_BOVIN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cgi ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cgi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Variants of the human pancreatic secretory trypsin inhibitor (PSTI) have been created during a protein design project to generate a high-affinity inhibitor with respect to some serine proteases other than trypsin. Two modified versions of human PSTI with high affinity for chymotrypsin were crystallized as a complex with chymotrypsinogen. Both crystallize isomorphously in space group P4(1)2(1)2 with lattice constants a = 84.4 A, c = 86.7 A and diffract to 2.3 A resolution. The structure was solved by molecular replacement. The final R-value after refinement with 8.0 to 2.3 A resolution data was 19.5% for both complexes after inclusion of about 50 bound water molecules. The overall three-dimensional structure of PSTI is similar to the structure of porcine PSTI in the trypsinogen complex (1TGS). Small differences in the relative orientation of the binding loop and the core of the inhibitors indicate flexible adaptation to the proteases. The chymotrypsinogen part of the complex is similar to chymotrypsin. After refolding induced by binding of the inhibitor the root-mean-square difference of the active site residues A186 to A195 and A217 to A222 compared to chymotrypsin was 0.26 A.

	Three-dimensional structure of the complexes between bovine chymotrypsinogen A and two recombinant variants of human pancreatic secretory trypsin inhibitor (Kazal-type).,Hecht HJ, Szardenings M, Collins J, Schomburg D J Mol Biol. 1991 Aug 5;220(3):711-22. PMID:1870127<ref>PMID:1870127</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1cgi" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Chymotrypsin 3D structures\|Chymotrypsin 3D structures]]		*[[Chymotrypsin 3D structures\|Chymotrypsin 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bovin~~]]		[[Category: Bos taurus]]
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Collins, J]]		[[Category: Collins J]]
	[[Category: Hecht~~, H J~~]]		[[Category: Hecht HJ]]
	[[Category: Schomburg, D]]		[[Category: Schomburg D]]
	[[Category: Szardenings, M]]		[[Category: Szardenings M]]
	~~[[Category: Serine protease-inhibitor complex]]~~
	~~[[Category: Serine protease-inhibitor complex complex~~]]

OCA at 10:36, 14 July 2021

2021-07-14T10:36:46Z

← Older revision		Revision as of 10:36, 14 July 2021
Line 3:		Line 3:
	<StructureSection load='1cgi' size='340' side='right'caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cgi' size='340' side='right'caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin] and [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CGI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CGI FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgi OCA], [~~http~~://pdbe.org/1cgi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cgi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cgi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cgi ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgi OCA], [https://pdbe.org/1cgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cgi RCSB], [https://www.ebi.ac.uk/pdbsum/1cgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cgi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 06:49, 10 October 2019

2019-10-10T06:49:09Z

← Older revision		Revision as of 06:49, 10 October 2019
Line 1:		Line 1:

	==THREE-DIMENSIONAL STRUCTURE OF THE COMPLEXES BETWEEN BOVINE CHYMOTRYPSINOGEN*A AND TWO RECOMBINANT VARIANTS OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL-TYPE)==		==THREE-DIMENSIONAL STRUCTURE OF THE COMPLEXES BETWEEN BOVINE CHYMOTRYPSINOGEN*A AND TWO RECOMBINANT VARIANTS OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL-TYPE)==
	<StructureSection load='1cgi' size='340' side='right' caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cgi' size='340' side='right'caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CGI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CGI FirstGlance]. <br>
Line 10:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/1cgi_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/1cgi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 25:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1cgi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cgi" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Chymotrypsin 3D structures\|Chymotrypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 34:
	[[Category: Bovin]]		[[Category: Bovin]]
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Collins, J]]		[[Category: Collins, J]]
	[[Category: Hecht, H J]]		[[Category: Hecht, H J]]

OCA at 08:11, 29 November 2017

2017-11-29T08:11:16Z

← Older revision		Revision as of 08:11, 29 November 2017
Line 1:		Line 1:

	==THREE-DIMENSIONAL STRUCTURE OF THE COMPLEXES BETWEEN BOVINE CHYMOTRYPSINOGEN*A AND TWO RECOMBINANT VARIANTS OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL-TYPE)==		==THREE-DIMENSIONAL STRUCTURE OF THE COMPLEXES BETWEEN BOVINE CHYMOTRYPSINOGEN*A AND TWO RECOMBINANT VARIANTS OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL-TYPE)==
	<StructureSection load='1cgi' size='340' side='right' caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cgi' size='340' side='right' caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CGI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CGI FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgi OCA], [http://pdbe.org/1cgi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cgi RCSB], [http://www.ebi.ac.uk/pdbsum/1cgi PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgi OCA], [http://pdbe.org/1cgi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cgi RCSB], [http://www.ebi.ac.uk/pdbsum/1cgi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cgi ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1cgi" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cgi" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Chymotrypsin\|Chymotrypsin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 04:00, 8 February 2016

2016-02-08T04:00:29Z

← Older revision		Revision as of 04:00, 8 February 2016
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cgi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:39, 10 September 2015

2015-09-10T07:39:08Z

← Older revision		Revision as of 07:39, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1cgi' size='340' side='right' caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cgi' size='340' side='right' caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~] and [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CGI FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CGI FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cgi RCSB], [http://www.ebi.ac.uk/pdbsum/1cgi PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgi OCA], [http://pdbe.org/1cgi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cgi RCSB], [http://www.ebi.ac.uk/pdbsum/1cgi PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1cgi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bos taurus~~]]		[[Category: Bovin]]
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Collins, J]]		[[Category: Collins, J]]
	[[Category: Hecht, H J]]		[[Category: Hecht, H J]]

OCA at 09:44, 22 December 2014

2014-12-22T09:44:16Z

← Older revision		Revision as of 09:44, 22 December 2014
Line 1:		Line 1:
	~~{{STRUCTURE_1cgi\| PDB=1cgi \| SCENE= }}~~		==THREE-DIMENSIONAL STRUCTURE OF THE COMPLEXES BETWEEN BOVINE CHYMOTRYPSINOGEN*A AND TWO RECOMBINANT VARIANTS OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL-TYPE)==
	===THREE-DIMENSIONAL STRUCTURE OF THE COMPLEXES BETWEEN BOVINE CHYMOTRYPSINOGEN*A AND TWO RECOMBINANT VARIANTS OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL-TYPE)===		<StructureSection load='1cgi' size='340' side='right' caption='[[1cgi]], [[Resolution\|resolution]] 2.30Å' scene=''>
	~~{{ABSTRACT_PUBMED_1870127}}~~		== Structural highlights ==
			<table><tr><td colspan='2'>[[1cgi]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CGI FirstGlance]. <br>
			</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cgi RCSB], [http://www.ebi.ac.uk/pdbsum/1cgi PDBsum]</span></td></tr>
			</table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/1cgi_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Variants of the human pancreatic secretory trypsin inhibitor (PSTI) have been created during a protein design project to generate a high-affinity inhibitor with respect to some serine proteases other than trypsin. Two modified versions of human PSTI with high affinity for chymotrypsin were crystallized as a complex with chymotrypsinogen. Both crystallize isomorphously in space group P4(1)2(1)2 with lattice constants a = 84.4 A, c = 86.7 A and diffract to 2.3 A resolution. The structure was solved by molecular replacement. The final R-value after refinement with 8.0 to 2.3 A resolution data was 19.5% for both complexes after inclusion of about 50 bound water molecules. The overall three-dimensional structure of PSTI is similar to the structure of porcine PSTI in the trypsinogen complex (1TGS). Small differences in the relative orientation of the binding loop and the core of the inhibitors indicate flexible adaptation to the proteases. The chymotrypsinogen part of the complex is similar to chymotrypsin. After refolding induced by binding of the inhibitor the root-mean-square difference of the active site residues A186 to A195 and A217 to A222 compared to chymotrypsin was 0.26 A.

	~~==About this Structure==~~		Three-dimensional structure of the complexes between bovine chymotrypsinogen A and two recombinant variants of human pancreatic secretory trypsin inhibitor (Kazal-type).,Hecht HJ, Szardenings M, Collins J, Schomburg D J Mol Biol. 1991 Aug 5;220(3):711-22. PMID:1870127<ref>PMID:1870127</ref>
	~~[[1cgi]] is a 2 chain~~ structure ~~with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus] and [http~~:~~//en~~.~~wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1CGI OCA].~~

	==~~Reference~~==		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	~~<ref group~~=~~"xtra">PMID:001870127</ref>~~<references ~~group="xtra"~~/><~~references~~/>		</div>

			==See Also==
			*[[Chymotrypsin\|Chymotrypsin]]
			== References ==
			<references/>
			__TOC__
			</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Collins, J.]]		[[Category: Collins, J]]
	[[Category: Hecht, H J.]]		[[Category: Hecht, H J]]
	[[Category: Schomburg, D.]]		[[Category: Schomburg, D]]
	[[Category: Szardenings, M.]]		[[Category: Szardenings, M]]
	[[Category: Serine protease-inhibitor complex]]		[[Category: Serine protease-inhibitor complex]]
	[[Category: Serine protease-inhibitor complex complex]]		[[Category: Serine protease-inhibitor complex complex]]

OCA at 04:26, 25 March 2013

2013-03-25T04:26:35Z

← Older revision		Revision as of 04:26, 25 March 2013
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	~~[[Image:1cgi.png\|left\|200px]]~~

	{{STRUCTURE_1cgi\| PDB=1cgi \| SCENE= }}		{{STRUCTURE_1cgi\| PDB=1cgi \| SCENE= }}

	===THREE-DIMENSIONAL STRUCTURE OF THE COMPLEXES BETWEEN BOVINE CHYMOTRYPSINOGEN*A AND TWO RECOMBINANT VARIANTS OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL-TYPE)===		===THREE-DIMENSIONAL STRUCTURE OF THE COMPLEXES BETWEEN BOVINE CHYMOTRYPSINOGEN*A AND TWO RECOMBINANT VARIANTS OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL-TYPE)===

	{{ABSTRACT_PUBMED_1870127}}		{{ABSTRACT_PUBMED_1870127}}

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	==Reference==		==Reference==
	<ref group="xtra">PMID:001870127</ref><references group="xtra"/>		<ref group="xtra">PMID:001870127</ref><references group="xtra"/><references/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]