1gpu - Revision history

OCA at 12:03, 13 December 2023

2023-12-13T12:03:07Z

← Older revision		Revision as of 12:03, 13 December 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gpu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GPU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gpu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GPU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THD:2-[3-[(4-AMINO-2-METHYL-5-PYRIMIDINYL)METHYL]-2-(1,2-DIHYDROXYETHYL)-4-METHYL-1,3-THIAZOL-3-IUM-5-YL]ETHYL+TRIHYDROGEN+DIPHOSPHATE'>THD</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.86Å</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THD:2-[3-[(4-AMINO-2-METHYL-5-PYRIMIDINYL)METHYL]-2-(1,2-DIHYDROXYETHYL)-4-METHYL-1,3-THIAZOL-3-IUM-5-YL]ETHYL+TRIHYDROGEN+DIPHOSPHATE'>THD</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [https://pdbe.org/1gpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [https://www.ebi.ac.uk/pdbsum/1gpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [https://pdbe.org/1gpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [https://www.ebi.ac.uk/pdbsum/1gpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/TKT1_YEAST TKT1_YEAST]] Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.<ref>PMID:8521838</ref>		[https://www.uniprot.org/uniprot/TKT1_YEAST TKT1_YEAST] Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.<ref>PMID:8521838</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Transketolase\|Transketolase]]		*[[Transketolase 3D structures\|Transketolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	~~[[Category: Transketolase]]~~		[[Category: Fiedler E]]
	[[Category: Fiedler, E]]		[[Category: Koenig S]]
	[[Category: Koenig, S]]		[[Category: Sandalova T]]
	[[Category: Sandalova, T]]		[[Category: Schneider G]]
	[[Category: Schneider, G]]		[[Category: Thorell S]]
	[[Category: Thorell, S]]
	~~[[Category: Transferase~~]]

OCA at 05:47, 28 April 2021

2021-04-28T05:47:27Z

← Older revision		Revision as of 05:47, 28 April 2021
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	<StructureSection load='1gpu' size='340' side='right'caption='[[1gpu]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='1gpu' size='340' side='right'caption='[[1gpu]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gpu]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1GPU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gpu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GPU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THD:2-[3-[(4-AMINO-2-METHYL-5-PYRIMIDINYL)METHYL]-2-(1,2-DIHYDROXYETHYL)-4-METHYL-1,3-THIAZOL-3-IUM-5-YL]ETHYL+TRIHYDROGEN+DIPHOSPHATE'>THD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THD:2-[3-[(4-AMINO-2-METHYL-5-PYRIMIDINYL)METHYL]-2-(1,2-DIHYDROXYETHYL)-4-METHYL-1,3-THIAZOL-3-IUM-5-YL]ETHYL+TRIHYDROGEN+DIPHOSPHATE'>THD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [~~http~~://pdbe.org/1gpu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1gpu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [https://pdbe.org/1gpu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [https://www.ebi.ac.uk/pdbsum/1gpu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/TKT1_YEAST TKT1_YEAST]] Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.<ref>PMID:8521838</ref>		[[https://www.uniprot.org/uniprot/TKT1_YEAST TKT1_YEAST]] Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.<ref>PMID:8521838</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:40, 24 July 2019

2019-07-24T06:40:25Z

← Older revision		Revision as of 06:40, 24 July 2019
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	==Transketolase complex with reaction intermediate==		==Transketolase complex with reaction intermediate==
	<StructureSection load='1gpu' size='340' side='right' caption='[[1gpu]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='1gpu' size='340' side='right'caption='[[1gpu]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gpu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GPU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gpu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GPU FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 1gpu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gpu" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Transketolase\|Transketolase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Transketolase]]		[[Category: Transketolase]]

OCA at 06:53, 3 January 2018

2018-01-03T06:53:43Z

← Older revision		Revision as of 06:53, 3 January 2018
Line 1:		Line 1:
	==Transketolase intermediate==
			==Transketolase complex with reaction intermediate==
	<StructureSection load='1gpu' size='340' side='right' caption='[[1gpu]], [[Resolution\|resolution]] 1.86Å' scene=''>		<StructureSection load='1gpu' size='340' side='right' caption='[[1gpu]], [[Resolution\|resolution]] 1.86Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [http://pdbe.org/1gpu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [http://www.ebi.ac.uk/pdbsum/1gpu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [http://pdbe.org/1gpu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [http://www.ebi.ac.uk/pdbsum/1gpu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gp/1gpu_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gp/1gpu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1gpu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gpu" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Transketolase\|Transketolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:22, 10 February 2016

2016-02-10T02:22:07Z

← Older revision		Revision as of 02:22, 10 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gpu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:26, 11 September 2015

2015-09-11T04:26:05Z

← Older revision		Revision as of 04:26, 11 September 2015
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [http://www.ebi.ac.uk/pdbsum/1gpu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [http://pdbe.org/1gpu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [http://www.ebi.ac.uk/pdbsum/1gpu PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1gpu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 11:07, 25 December 2014

2014-12-25T11:07:12Z

← Older revision		Revision as of 11:07, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [http://www.ebi.ac.uk/pdbsum/1gpu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [http://www.ebi.ac.uk/pdbsum/1gpu PDBsum]</span></td></tr>
	</table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/TKT1_YEAST TKT1_YEAST]] Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.<ref>PMID:8521838</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 00:47, 23 December 2014

2014-12-23T00:47:56Z

← Older revision		Revision as of 00:47, 23 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gpu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GPU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gpu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GPU FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THD:2-[3-[(4-AMINO-2-METHYL-5-PYRIMIDINYL)METHYL]-2-(1,2-DIHYDROXYETHYL)-4-METHYL-1,3-THIAZOL-3-IUM-5-YL]ETHYL+TRIHYDROGEN+DIPHOSPHATE'>THD</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THD:2-[3-[(4-AMINO-2-METHYL-5-PYRIMIDINYL)METHYL]-2-(1,2-DIHYDROXYETHYL)-4-METHYL-1,3-THIAZOL-3-IUM-5-YL]ETHYL+TRIHYDROGEN+DIPHOSPHATE'>THD</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [http://www.ebi.ac.uk/pdbsum/1gpu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [http://www.ebi.ac.uk/pdbsum/1gpu PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Transketolase]]		[[Category: Transketolase]]
	[[Category: Fiedler, E.]]		[[Category: Fiedler, E]]
	[[Category: Koenig, S.]]		[[Category: Koenig, S]]
	[[Category: Sandalova, T.]]		[[Category: Sandalova, T]]
	[[Category: Schneider, G.]]		[[Category: Schneider, G]]
	[[Category: Thorell, S.]]		[[Category: Thorell, S]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:39, 28 September 2014

2014-09-28T14:39:47Z

← Older revision		Revision as of 14:39, 28 September 2014
Line 1:		Line 1:
	[[Image:~~1gpu~~.~~png~~\|~~left~~\|~~200px~~]]		==Transketolase intermediate==
			<StructureSection load='1gpu' size='340' side='right' caption='[[1gpu]], [[Resolution\|resolution]] 1.86Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1gpu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GPU FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=THD:2-[3-[(4-AMINO-2-METHYL-5-PYRIMIDINYL)METHYL]-2-(1,2-DIHYDROXYETHYL)-4-METHYL-1,3-THIAZOL-3-IUM-5-YL]ETHYL+TRIHYDROGEN+DIPHOSPHATE'>THD</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ay0\|1ay0]], [[1ngs\|1ngs]], [[1tka\|1tka]], [[1tkb\|1tkb]], [[1tkc\|1tkc]], [[1trk\|1trk]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transketolase Transketolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.1 2.2.1.1] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gpu RCSB], [http://www.ebi.ac.uk/pdbsum/1gpu PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gp/1gpu_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Kinetic and spectroscopic data indicated that addition of the donor substrate hydroxypyruvate to the thiamin diphosphate (ThDP)-dependent enzyme transketolase (TK) led to the accumulation of the alpha-carbanion/enamine of (alpha,beta-dihydroxyethyl) ThDP, the key reaction intermediate in enzymatic thiamin catalysis. The three-dimensional structure of this intermediate trapped in the active site of yeast TK was determined to 1.9-A resolution by using cryocrystallography. The electron density suggests a planar alpha-carbanion/enamine intermediate having the E-configuration. The reaction intermediate is firmly held in place through direct hydrogen bonds to His-103 and His-481 and an indirect hydrogen bond via a water molecule to His-69. The 4-NH(2) group of the amino-pyrimidine ring of ThDP is within 3 A distance to the alpha-hydroxy oxygen atom of the dihydroxyethyl moiety but at an angle unfavorable for a strong hydrogen bond. No structural changes occur in TK on formation of the reaction intermediate, suggesting that the active site is poised for catalysis and conformational changes during the enzyme reaction are not very likely. The intermediate is present with high occupancy in both active sites, arguing against previous proposals of half-of-the-sites reactivity in yeast TK.

	~~{{STRUCTURE_1gpu\| PDB=1gpu \| SCENE= }}~~		Snapshot of a key intermediate in enzymatic thiamin catalysis: crystal structure of the alpha-carbanion of (alpha,beta-dihydroxyethyl)-thiamin diphosphate in the active site of transketolase from Saccharomyces cerevisiae.,Fiedler E, Thorell S, Sandalova T, Golbik R, Konig S, Schneider G Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):591-5. Epub 2002 Jan 2. PMID:11773632<ref>PMID:11773632</ref>

	~~===Transketolase intermediate===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_11773632}}~~

	~~==About this Structure==~~
	~~[[1gpu]] is~~ a ~~2 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann.ac.il/oca-bin~~/~~ocashort?id=1GPU OCA].~~

	==See Also==		==See Also==
	*[[Transketolase\|Transketolase]]		*[[Transketolase\|Transketolase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:011773632<~~/~~ref~~><~~ref group="xtra">PMID:009398292</ref><ref group="xtra">PMID:009119035</ref><ref group="xtra">PMID:008176731</ref><ref group="xtra">PMID:012651851</ref><references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Transketolase]]		[[Category: Transketolase]]

OCA at 15:59, 20 October 2012

2012-10-20T15:59:29Z

← Older revision		Revision as of 15:59, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[1gpu]] is a 2 chain structure ~~of [[Transketolase]]~~ with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA].		[[1gpu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPU OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
	<ref group="xtra">PMID:011773632</ref><ref group="xtra">PMID:009398292</ref><ref group="xtra">PMID:009119035~~</ref><ref group="xtra">PMID:008974393~~</ref><ref group="xtra">PMID:008176731</ref><ref group="xtra">PMID:012651851</ref><references group="xtra"/>		<ref group="xtra">PMID:011773632</ref><ref group="xtra">PMID:009398292</ref><ref group="xtra">PMID:009119035</ref><ref group="xtra">PMID:008176731</ref><ref group="xtra">PMID:012651851</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Transketolase]]		[[Category: Transketolase]]