1imo - Revision history

OCA at 08:35, 22 May 2024

2024-05-22T08:35:34Z

← Older revision		Revision as of 08:35, 22 May 2024
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1imo ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1imo ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			BRCT (BRCA1 carboxyl terminus) domains are found in a number of DNA repair enzymes and cell cycle regulators and are believed to mediate important protein-protein interactions. The DNA ligase IIIalpha BRCT domain partners with the distal BRCT domain of the DNA repair protein XRCC1 (X1BRCTb) in the DNA base excision repair (BER) pathway. To elucidate the mechanisms by which these two domains can interact, we have determined the solution structure of human ligase IIIalpha BRCT (L3[86], residues 837-922). The structure of L3[86] consists of a beta2beta1beta3beta4 parallel sheet with a two-alpha-helix bundle packed against one face of the sheet. This fold is conserved in several proteins having a wide range of activities, including X1BRCTb [Zhang, X. D., et al. (1998) EMBO J. 17, 6404-6411]. L3[86] exists as a dimer in solution, but an insufficient number of NOE restraints precluded the determination of the homodimer structure. However, 13C isotope-filtered and hydrogen-deuterium exchange experiments indicate that the N-terminus, alpha1, the alpha1-beta2 loop, and the three residues following alpha2 are involved in forming the dimer interface, as similarly observed in the structure of X1BRCTb. NOE and dynamic data indicate that several residues (837-844) in the N-terminal region appear to interconvert between helix and random coil conformations. Further studies of other BRCT domains and of their complexes are needed to address how these proteins interact with one another, and to shed light on how mutations can lead to disruption of function and ultimately disease.

			Solution structure and backbone dynamics of the human DNA ligase IIIalpha BRCT domain.,Krishnan VV, Thornton KH, Thelen MP, Cosman M Biochemistry. 2001 Nov 6;40(44):13158-66. PMID:11683624<ref>PMID:11683624</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 1imo" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[DNA ligase 3D structures\|DNA ligase 3D structures]]		*[[DNA ligase 3D structures\|DNA ligase 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:48, 3 April 2024

2024-04-03T07:48:08Z

← Older revision		Revision as of 07:48, 3 April 2024
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	==NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN==		==NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN==
	<StructureSection load='1imo' size='340' side='right'caption='[[1imo~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>		<StructureSection load='1imo' size='340' side='right'caption='[[1imo]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IMO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IMO FirstGlance]. <br>
	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'>[https://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span~~></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [https://pdbe.org/1imo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [https://www.ebi.ac.uk/pdbsum/1imo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1imo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [https://pdbe.org/1imo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [https://www.ebi.ac.uk/pdbsum/1imo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1imo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/DNLI3_HUMAN DNLI3_HUMAN]] Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.		[https://www.uniprot.org/uniprot/DNLI3_HUMAN DNLI3_HUMAN] Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1imo ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1imo ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	BRCT (BRCA1 carboxyl terminus) domains are found in a number of DNA repair enzymes and cell cycle regulators and are believed to mediate important protein-protein interactions. The DNA ligase IIIalpha BRCT domain partners with the distal BRCT domain of the DNA repair protein XRCC1 (X1BRCTb) in the DNA base excision repair (BER) pathway. To elucidate the mechanisms by which these two domains can interact, we have determined the solution structure of human ligase IIIalpha BRCT (L3[86], residues 837-922). The structure of L3[86] consists of a beta2beta1beta3beta4 parallel sheet with a two-alpha-helix bundle packed against one face of the sheet. This fold is conserved in several proteins having a wide range of activities, including X1BRCTb [Zhang, X. D., et al. (1998) EMBO J. 17, 6404-6411]. L3[86] exists as a dimer in solution, but an insufficient number of NOE restraints precluded the determination of the homodimer structure. However, 13C isotope-filtered and hydrogen-deuterium exchange experiments indicate that the N-terminus, alpha1, the alpha1-beta2 loop, and the three residues following alpha2 are involved in forming the dimer interface, as similarly observed in the structure of X1BRCTb. NOE and dynamic data indicate that several residues (837-844) in the N-terminal region appear to interconvert between helix and random coil conformations. Further studies of other BRCT domains and of their complexes are needed to address how these proteins interact with one another, and to shed light on how mutations can lead to disruption of function and ultimately disease.

	Solution structure and backbone dynamics of the human DNA ligase IIIalpha BRCT domain.,Krishnan VV, Thornton KH, Thelen MP, Cosman M Biochemistry. 2001 Nov 6;40(44):13158-66. PMID:11683624<ref>PMID:11683624</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1imo" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[DNA ligase 3D structures\|DNA ligase 3D structures]]		*[[DNA ligase 3D structures\|DNA ligase 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cosman, M]]		[[Category: Cosman M]]
	[[Category: Krishnan~~, V V~~]]		[[Category: Krishnan VV]]
	[[Category: Thelen~~, M P~~]]		[[Category: Thelen MP]]
	[[Category: Thornton~~, K H]]~~		[[Category: Thornton KH]]
	~~[[Category: Ligase]]~~
	~~[[Category: Parallel beta sheet~~]]

OCA at 11:02, 4 August 2021

2021-08-04T11:02:18Z

← Older revision		Revision as of 11:02, 4 August 2021
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	<StructureSection load='1imo' size='340' side='right'caption='[[1imo]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1imo' size='340' side='right'caption='[[1imo]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1IMO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IMO FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [~~http~~://pdbe.org/1imo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1imo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1imo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [https://pdbe.org/1imo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [https://www.ebi.ac.uk/pdbsum/1imo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1imo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/DNLI3_HUMAN DNLI3_HUMAN]] Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.		[[https://www.uniprot.org/uniprot/DNLI3_HUMAN DNLI3_HUMAN]] Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	==See Also==		==See Also==
	*[[DNA ligase 3D structures\|DNA ligase 3D structures]]		*[[DNA ligase 3D structures\|DNA ligase 3D structures]]
	*[[PDB identification code\|PDB identification code]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:59, 30 October 2019

2019-10-30T09:59:03Z

← Older revision		Revision as of 09:59, 30 October 2019
Line 1:		Line 1:

	==NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN==		==NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN==
	<StructureSection load='1imo' size='340' side='right' caption='[[1imo]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1imo' size='340' side='right'caption='[[1imo]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMO FirstGlance]. <br>
Line 30:		Line 30:

	==See Also==		==See Also==
			*[[DNA ligase 3D structures\|DNA ligase 3D structures]]
	*[[PDB identification code\|PDB identification code]]		*[[PDB identification code\|PDB identification code]]
	== References ==		== References ==
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Cosman, M]]		[[Category: Cosman, M]]
	[[Category: Krishnan, V V]]		[[Category: Krishnan, V V]]

OCA at 08:59, 10 January 2018

2018-01-10T08:59:10Z

← Older revision		Revision as of 08:59, 10 January 2018
Line 1:		Line 1:

	==NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN==		==NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN==
	<StructureSection load='1imo' size='340' side='right' caption='[[1imo]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1imo' size='340' side='right' caption='[[1imo]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
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	<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMO FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [http://pdbe.org/1imo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [http://www.ebi.ac.uk/pdbsum/1imo PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [http://pdbe.org/1imo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [http://www.ebi.ac.uk/pdbsum/1imo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1imo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/1imo_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/1imo_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:

	==See Also==		==See Also==
	*[[DNA ligase\|DNA ligase]]
	*[[PDB identification code\|PDB identification code]]		*[[PDB identification code\|PDB identification code]]
	== References ==		== References ==

OCA at 12:28, 7 February 2016

2016-02-07T12:28:18Z

← Older revision		Revision as of 12:28, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1imo ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:30, 10 September 2015

2015-09-10T01:30:41Z

← Older revision		Revision as of 01:30, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1imo' size='340' side='right' caption='[[1imo]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1imo' size='340' side='right' caption='[[1imo]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMO FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [http://www.ebi.ac.uk/pdbsum/1imo PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [http://pdbe.org/1imo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [http://www.ebi.ac.uk/pdbsum/1imo PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1imo" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Cosman, M]]		[[Category: Cosman, M]]
	[[Category: Krishnan, V V]]		[[Category: Krishnan, V V]]

OCA at 15:06, 24 December 2014

2014-12-24T15:06:17Z

← Older revision		Revision as of 15:06, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMO FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [http://www.ebi.ac.uk/pdbsum/1imo PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [http://www.ebi.ac.uk/pdbsum/1imo PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/DNLI3_HUMAN DNLI3_HUMAN]] Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cosman, M.]]		[[Category: Cosman, M]]
	[[Category: Krishnan, V V.]]		[[Category: Krishnan, V V]]
	[[Category: Thelen, M P.]]		[[Category: Thelen, M P]]
	[[Category: Thornton, K H.]]		[[Category: Thornton, K H]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Parallel beta sheet]]		[[Category: Parallel beta sheet]]

OCA at 12:29, 28 September 2014

2014-09-28T12:29:34Z

← Older revision		Revision as of 12:29, 28 September 2014
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	[[Image:~~1imo~~.~~png~~\|~~left~~\|~~200px~~]]		==NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN==
			<StructureSection load='1imo' size='340' side='right' caption='[[1imo]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IMO FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1imo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1imo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1imo RCSB], [http://www.ebi.ac.uk/pdbsum/1imo PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/im/1imo_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			BRCT (BRCA1 carboxyl terminus) domains are found in a number of DNA repair enzymes and cell cycle regulators and are believed to mediate important protein-protein interactions. The DNA ligase IIIalpha BRCT domain partners with the distal BRCT domain of the DNA repair protein XRCC1 (X1BRCTb) in the DNA base excision repair (BER) pathway. To elucidate the mechanisms by which these two domains can interact, we have determined the solution structure of human ligase IIIalpha BRCT (L3[86], residues 837-922). The structure of L3[86] consists of a beta2beta1beta3beta4 parallel sheet with a two-alpha-helix bundle packed against one face of the sheet. This fold is conserved in several proteins having a wide range of activities, including X1BRCTb [Zhang, X. D., et al. (1998) EMBO J. 17, 6404-6411]. L3[86] exists as a dimer in solution, but an insufficient number of NOE restraints precluded the determination of the homodimer structure. However, 13C isotope-filtered and hydrogen-deuterium exchange experiments indicate that the N-terminus, alpha1, the alpha1-beta2 loop, and the three residues following alpha2 are involved in forming the dimer interface, as similarly observed in the structure of X1BRCTb. NOE and dynamic data indicate that several residues (837-844) in the N-terminal region appear to interconvert between helix and random coil conformations. Further studies of other BRCT domains and of their complexes are needed to address how these proteins interact with one another, and to shed light on how mutations can lead to disruption of function and ultimately disease.

	~~{{STRUCTURE_1imo\| PDB=1imo \| SCENE= }}~~		Solution structure and backbone dynamics of the human DNA ligase IIIalpha BRCT domain.,Krishnan VV, Thornton KH, Thelen MP, Cosman M Biochemistry. 2001 Nov 6;40(44):13158-66. PMID:11683624<ref>PMID:11683624</ref>

	~~===NMR STRUCTURE OF HUMAN DNA LIGASE IIIALPHA BRCT DOMAIN===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_11683624}}~~

	~~==About this Structure==~~
	~~[[1imo]] is~~ a ~~1 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http://oca~~.~~weizmann.ac.il/oca-bin~~/~~ocashort?id=1IMO OCA].~~

	==See Also==		==See Also==
	*[[DNA ligase\|DNA ligase]]		*[[DNA ligase\|DNA ligase]]
			*[[PDB identification code\|PDB identification code]]
	==~~Reference~~==		== References ==
	<~~ref group="xtra">PMID:011683624<~~/~~ref~~><~~ref group="xtra">PMID:017915942</ref><references group="xtra"~~/>		<references/>
			__TOC__
			</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Cosman, M.]]		[[Category: Cosman, M.]]

OCA at 06:38, 20 October 2012

2012-10-20T06:38:52Z

← Older revision		Revision as of 06:38, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[1imo]] is a 1 chain structure ~~of [[DNA ligase]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA].		[[1imo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMO OCA].

	==See Also==		==See Also==