1ji6 - Revision history

OCA at 07:39, 7 February 2024

2024-02-07T07:39:38Z

← Older revision		Revision as of 07:39, 7 February 2024
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	<StructureSection load='1ji6' size='340' side='right'caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ji6' size='340' side='right'caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var.~~ thuringiensis~~" smith et al. 1952~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>~~[[~~1dlc~~\|~~1dlc~~]]~~</div>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [https://pdbe.org/1ji6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ji6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ji6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [https://pdbe.org/1ji6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ji6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ji6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/CR3BB_BACTU CR3BB_BACTU]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera. Has moderate level of toxicity to southern corn rootworm.		[https://www.uniprot.org/uniprot/CR3BB_BACTU CR3BB_BACTU] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera. Has moderate level of toxicity to southern corn rootworm.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ji6 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ji6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The coleopteran-active delta-endotoxin Cry3Bb1 from Bacillus thuringiensis (Bt) strain EG7231 is uniquely toxic to Diabrotica undecimpunctata, the Southern corn rootworm, while retaining activity against Leptinotarsa decemlineata, the Colorado potato beetle. The crystal structure of the delta-endotoxin Cry3Bb1 has been refined using data collected to 2.4 A resolution, with a residual R factor of 17.5% and an R(free) of 25.3%. The structure is made up of three domains: I, a seven-helix bundle (residues 64-294); II, a three-sheet domain (residues 295-502); and III, a beta-sandwich domain (residues 503-652). The monomers in the orthorhombic C222(1) crystal lattice form a dimeric quaternary structure across a crystallographic twofold axis, with a channel formed involving interactions between domains I and III. There are 23 hydrogen bonds between the two monomers conferring structural stability on the dimer. It has been demonstrated that Cry3Bb1 and the similar toxin Cry3A form oligomers in solution. The structural results presented here indicate that the interactions between domains I and III could be responsible for the initial higher order structure and have implications for the biological activity of these toxins. There are seven additional single amino-acid residues in the sequence of Cry3Bb1 compared with that of Cry3A; one in domain I, two in domain II and four in domain III, which also shows the largest conformational difference between the two proteins. These changes can be implicated in the selectivity differences noted for these two delta-endotoxins.

	Structure of the insecticidal bacterial delta-endotoxin Cry3Bb1 of Bacillus thuringiensis.,Galitsky N, Cody V, Wojtczak A, Ghosh D, Luft JR, Pangborn W, English L Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1101-9. Epub 2001, Jul 23. PMID:11468393<ref>PMID:11468393</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1ji6" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Delta-endotoxin\|Delta-endotoxin]]		*[[Delta-endotoxin\|Delta-endotoxin]]
	*[[Pesticidal crystal protein\|Pesticidal crystal protein]]		*[[Pesticidal crystal protein\|Pesticidal crystal protein]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bacillus ~~cereus var.~~ thuringiensis ~~smith et al. 1952~~]]		[[Category: Bacillus thuringiensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cody, V]]		[[Category: Cody V]]
	~~[[Category: Cry3bb1]]~~
	~~[[Category: Toxin~~]]

OCA at 07:19, 14 April 2021

2021-04-14T07:19:16Z

← Older revision		Revision as of 07:19, 14 April 2021
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	<StructureSection load='1ji6' size='340' side='right'caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ji6' size='340' side='right'caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dlc\|1dlc]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dlc\|1dlc]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [~~http~~://pdbe.org/1ji6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ji6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ji6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [https://pdbe.org/1ji6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ji6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ji6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/CR3BB_BACTU CR3BB_BACTU]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera. Has moderate level of toxicity to southern corn rootworm.		[[https://www.uniprot.org/uniprot/CR3BB_BACTU CR3BB_BACTU]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera. Has moderate level of toxicity to southern corn rootworm.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:55, 10 July 2019

2019-07-10T06:55:54Z

← Older revision		Revision as of 06:55, 10 July 2019
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	==CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS==		==CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS==
	<StructureSection load='1ji6' size='340' side='right' caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ji6' size='340' side='right'caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/1ji6_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/1ji6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus cereus var. thuringiensis smith et al. 1952]]		[[Category: Bacillus cereus var. thuringiensis smith et al. 1952]]
			[[Category: Large Structures]]
	[[Category: Cody, V]]		[[Category: Cody, V]]
	[[Category: Cry3bb1]]		[[Category: Cry3bb1]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 09:47, 4 October 2017

2017-10-04T09:47:08Z

← Older revision		Revision as of 09:47, 4 October 2017
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	==CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS==		==CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS==
	<StructureSection load='1ji6' size='340' side='right' caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ji6' size='340' side='right' caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dlc\|1dlc]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dlc\|1dlc]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [http://pdbe.org/1ji6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ji6 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [http://pdbe.org/1ji6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ji6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ji6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ji6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Delta-endotoxin\|Delta-endotoxin]]		*[[Delta-endotoxin\|Delta-endotoxin]]
			*[[Pesticidal crystal protein\|Pesticidal crystal protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:17, 11 September 2015

2015-09-11T08:17:56Z

← Older revision		Revision as of 08:17, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ji6' size='340' side='right' caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='1ji6' size='340' side='right' caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_thuringiensis Bacillus~~ thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_cereus_var._thuringiensis"_smith_et_al._1952 "bacillus cereus var. thuringiensis" smith et al. 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dlc\|1dlc]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dlc\|1dlc]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ji6 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [http://pdbe.org/1ji6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ji6 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1ji6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bacillus thuringiensis]]		[[Category: Bacillus cereus var. thuringiensis smith et al. 1952]]
	[[Category: Cody, V]]		[[Category: Cody, V]]
	[[Category: Cry3bb1]]		[[Category: Cry3bb1]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 13:45, 25 December 2014

2014-12-25T13:45:22Z

← Older revision		Revision as of 13:45, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dlc\|1dlc]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dlc\|1dlc]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ji6 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ji6 PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/CR3BB_BACTU CR3BB_BACTU]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of Coleoptera. Has moderate level of toxicity to southern corn rootworm.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus thuringiensis]]		[[Category: Bacillus thuringiensis]]
	[[Category: Cody, V.]]		[[Category: Cody, V]]
	[[Category: Cry3bb1]]		[[Category: Cry3bb1]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 12:29, 28 September 2014

2014-09-28T12:29:17Z

← Older revision		Revision as of 12:29, 28 September 2014
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	[[Image:~~1ji6~~.~~png~~\|~~left~~\|~~200px~~]]		==CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS==
			<StructureSection load='1ji6' size='340' side='right' caption='[[1ji6]], [[Resolution\|resolution]] 2.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JI6 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dlc\|1dlc]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ji6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ji6 RCSB], [http://www.ebi.ac.uk/pdbsum/1ji6 PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/1ji6_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The coleopteran-active delta-endotoxin Cry3Bb1 from Bacillus thuringiensis (Bt) strain EG7231 is uniquely toxic to Diabrotica undecimpunctata, the Southern corn rootworm, while retaining activity against Leptinotarsa decemlineata, the Colorado potato beetle. The crystal structure of the delta-endotoxin Cry3Bb1 has been refined using data collected to 2.4 A resolution, with a residual R factor of 17.5% and an R(free) of 25.3%. The structure is made up of three domains: I, a seven-helix bundle (residues 64-294); II, a three-sheet domain (residues 295-502); and III, a beta-sandwich domain (residues 503-652). The monomers in the orthorhombic C222(1) crystal lattice form a dimeric quaternary structure across a crystallographic twofold axis, with a channel formed involving interactions between domains I and III. There are 23 hydrogen bonds between the two monomers conferring structural stability on the dimer. It has been demonstrated that Cry3Bb1 and the similar toxin Cry3A form oligomers in solution. The structural results presented here indicate that the interactions between domains I and III could be responsible for the initial higher order structure and have implications for the biological activity of these toxins. There are seven additional single amino-acid residues in the sequence of Cry3Bb1 compared with that of Cry3A; one in domain I, two in domain II and four in domain III, which also shows the largest conformational difference between the two proteins. These changes can be implicated in the selectivity differences noted for these two delta-endotoxins.

	~~{{STRUCTURE_1ji6\| PDB=1ji6 \| SCENE= }}~~		Structure of the insecticidal bacterial delta-endotoxin Cry3Bb1 of Bacillus thuringiensis.,Galitsky N, Cody V, Wojtczak A, Ghosh D, Luft JR, Pangborn W, English L Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1101-9. Epub 2001, Jul 23. PMID:11468393<ref>PMID:11468393</ref>

	~~===CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_11468393}}~~

	~~==About this Structure==~~
	~~[[1ji6]] is~~ a ~~1 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann.ac.il/oca-bin~~/~~ocashort?id=1JI6 OCA].~~

	==See Also==		==See Also==
	*[[Delta-endotoxin\|Delta-endotoxin]]		*[[Delta-endotoxin\|Delta-endotoxin]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:011468393<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Bacillus thuringiensis]]		[[Category: Bacillus thuringiensis]]
	[[Category: Cody, V.]]		[[Category: Cody, V.]]
	[[Category: Cry3bb1]]		[[Category: Cry3bb1]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 17:24, 20 October 2012

2012-10-20T17:24:44Z

← Older revision		Revision as of 17:24, 20 October 2012
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	==About this Structure==		==About this Structure==
	[[1ji6]] is a 1 chain structure ~~of [[Delta-endotoxin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA].		[[1ji6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
	<ref group="xtra">PMID:011468393~~</ref><ref group="xtra">PMID:012527302~~</ref><references group="xtra"/>		<ref group="xtra">PMID:011468393</ref><references group="xtra"/>
	[[Category: Bacillus thuringiensis]]		[[Category: Bacillus thuringiensis]]
	[[Category: Cody, V.]]		[[Category: Cody, V.]]
	[[Category: Cry3bb1]]		[[Category: Cry3bb1]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA: Protected "1ji6" [edit=sysop:move=sysop]

2012-07-27T00:48:18Z

Protected "1ji6" [edit=sysop:move=sysop]

← Older revision	Revision as of 00:48, 27 July 2012
(No difference)

OCA at 00:48, 27 July 2012

2012-07-27T00:48:16Z

← Older revision		Revision as of 00:48, 27 July 2012
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	~~{{Seed}}~~
	[[Image:1ji6.png\|left\|200px]]		[[Image:1ji6.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_1ji6", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_1ji6\| PDB=1ji6 \| SCENE= }}		{{STRUCTURE_1ji6\| PDB=1ji6 \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS===		===CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_11468393}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 11468393 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_11468393}}		{{ABSTRACT_PUBMED_11468393}}

	==About this Structure==		==About this Structure==
	~~1JI6~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA].		[[1ji6]] is a 1 chain structure of [[Delta-endotoxin]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA].

			==See Also==
			*[[Delta-endotoxin\|Delta-endotoxin]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~11468393~~</ref><references group="xtra"/>		<ref group="xtra">PMID:011468393</ref><ref group="xtra">PMID:012527302</ref><references group="xtra"/>
	[[Category: Bacillus thuringiensis]]		[[Category: Bacillus thuringiensis]]
	[[Category: Cody, V.]]		[[Category: Cody, V.]]
	[[Category: Cry3bb1]]		[[Category: Cry3bb1]]
			[[Category: Toxin]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 17:47:37 2009''~~