1mvb - Revision history

OCA at 06:07, 3 April 2024

2024-04-03T06:07:51Z

← Older revision		Revision as of 06:07, 3 April 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVB FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [https://pdbe.org/1mvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [https://www.ebi.ac.uk/pdbsum/1mvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvb ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [https://pdbe.org/1mvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [https://www.ebi.ac.uk/pdbsum/1mvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mvb ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mvb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	In MS2 assembly of phage particles results from an interaction between a coat protein dimer and a stem-loop of the RNA genome (the operator hairpin). Amino acid residues Thr45, which is universally conserved among the small RNA phages, and Thr59 are part of the specific RNA binding pocket and interact directly with the RNA; the former through a hydrogen bond, the latter through hydrophobic contacts. The crystal structures of MS2 protein capsids formed by mutants Thr45Ala and Thr59Ser, both with and without the 19 nt wild-type operator hairpin bound, are reported here. The RNA hairpin binds to these mutants in a similar way to its binding to wild-type protein. In a companion paper both mutants are shown to be deficient in RNA binding in an in vivo assay, but in vitro the equilibrium dissociation constant is significantly higher than wild-type for the Thr45Ala mutant. The change in binding affinity of the Thr45Ala mutant is probably a direct consequence of removal of direct hydrogen bonds between the protein and the RNA. The properties of the Thr59Ser mutant are more difficult to explain, but are consistent with a loss of non-polar contact.

	Crystal structures of MS2 coat protein mutants in complex with wild-type RNA operator fragments.,van den Worm SH, Stonehouse NJ, Valegard K, Murray JB, Walton C, Fridborg K, Stockley PG, Liljas L Nucleic Acids Res. 1998 Mar 1;26(5):1345-51. PMID:9469847<ref>PMID:9469847</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1mvb" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:33, 19 April 2023

2023-04-19T09:33:42Z

← Older revision		Revision as of 09:33, 19 April 2023
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	<StructureSection load='1mvb' size='340' side='right'caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1mvb' size='340' side='right'caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacteriophage_ms2 Bacteriophage ms2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVB FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [https://pdbe.org/1mvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [https://www.ebi.ac.uk/pdbsum/1mvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvb ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [https://pdbe.org/1mvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [https://www.ebi.ac.uk/pdbsum/1mvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/~~COAT_BPMS2 COAT_BPMS2~~]~~] Forms~~ the ~~phage~~ shell~~; binds~~ to the ~~phage~~ RNA.		[https://www.uniprot.org/uniprot/CAPSD_BPMS2 CAPSD_BPMS2] Self-assembles to form the T=3 icosahedral virus shell that protects the viral nucleic acid. Acts as a translational repressor by binding with high specificity to a single stem-loop structure in the genomic RNA that contains the initiation codon of the gene for the viral replicase. Involved in virus assembly through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome.<ref>PMID:16531233</ref> <ref>PMID:18662904</ref> <ref>PMID:26608810</ref> <ref>PMID:8254664</ref> <ref>PMID:9245600</ref> <ref>PMID:9469847</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacteriophage ms2~~]]		[[Category: Escherichia phage MS2]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Liljas, L]]		[[Category: Liljas L]]
	[[Category: Stonehouse~~, N J~~]]		[[Category: Stonehouse NJ]]
	[[Category: Valegard, K]]		[[Category: Valegard K]]
	[[Category: Vandenworm, S]]		[[Category: Vandenworm S]]
	~~[[Category: Bacteriophage coat protein]]~~
	~~[[Category: Icosahedral virus]]~~
	~~[[Category: Virus~~]]

OCA at 09:30, 5 May 2021

2021-05-05T09:30:05Z

← Older revision		Revision as of 09:30, 5 May 2021
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	<StructureSection load='1mvb' size='340' side='right'caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1mvb' size='340' side='right'caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVB FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [~~http~~://pdbe.org/1mvb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1mvb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvb ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [https://pdbe.org/1mvb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [https://www.ebi.ac.uk/pdbsum/1mvb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/COAT_BPMS2 COAT_BPMS2]] Forms the phage shell; binds to the phage RNA.		[[https://www.uniprot.org/uniprot/COAT_BPMS2 COAT_BPMS2]] Forms the phage shell; binds to the phage RNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:02, 31 July 2019

2019-07-31T08:02:58Z

← Older revision		Revision as of 08:02, 31 July 2019
Line 1:		Line 1:

	==STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2==		==STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2==
	<StructureSection load='1mvb' size='340' side='right' caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1mvb' size='340' side='right'caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVB FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/1mvb_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/1mvb_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Bacteriophage ms2]]		[[Category: Bacteriophage ms2]]
			[[Category: Large Structures]]
	[[Category: Liljas, L]]		[[Category: Liljas, L]]
	[[Category: Stonehouse, N J]]		[[Category: Stonehouse, N J]]

OCA at 10:23, 25 October 2017

2017-10-25T10:23:50Z

← Older revision		Revision as of 10:23, 25 October 2017
Line 1:		Line 1:

	==STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2==		==STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2==
	<StructureSection load='1mvb' size='340' side='right' caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1mvb' size='340' side='right' caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVB FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [http://pdbe.org/1mvb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [http://www.ebi.ac.uk/pdbsum/1mvb PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [http://pdbe.org/1mvb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [http://www.ebi.ac.uk/pdbsum/1mvb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mvb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:36, 11 September 2015

2015-09-11T12:36:18Z

← Older revision		Revision as of 12:36, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1mvb' size='340' side='right' caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1mvb' size='340' side='right' caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacterio_phage_ms2 Enterobacterio phage~~ ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVB FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [http://www.ebi.ac.uk/pdbsum/1mvb PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [http://pdbe.org/1mvb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [http://www.ebi.ac.uk/pdbsum/1mvb PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1mvb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Enterobacterio phage~~ ms2]]		[[Category: Bacteriophage ms2]]
	[[Category: Liljas, L]]		[[Category: Liljas, L]]
	[[Category: Stonehouse, N J]]		[[Category: Stonehouse, N J]]

OCA at 16:31, 25 December 2014

2014-12-25T16:31:26Z

← Older revision		Revision as of 16:31, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVB FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [http://www.ebi.ac.uk/pdbsum/1mvb PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [http://www.ebi.ac.uk/pdbsum/1mvb PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/COAT_BPMS2 COAT_BPMS2]] Forms the phage shell; binds to the phage RNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacterio phage ms2]]		[[Category: Enterobacterio phage ms2]]
	[[Category: Liljas, L.]]		[[Category: Liljas, L]]
	[[Category: Stonehouse, N J.]]		[[Category: Stonehouse, N J]]
	[[Category: Valegard, K.]]		[[Category: Valegard, K]]
	[[Category: Vandenworm, S.]]		[[Category: Vandenworm, S]]
	[[Category: Bacteriophage coat protein]]		[[Category: Bacteriophage coat protein]]
	[[Category: Icosahedral virus]]		[[Category: Icosahedral virus]]
	[[Category: Virus]]		[[Category: Virus]]

OCA at 14:44, 28 September 2014

2014-09-28T14:44:06Z

← Older revision		Revision as of 14:44, 28 September 2014
Line 1:		Line 1:
	[[Image:~~1mvb~~.~~png~~\|~~left~~\|~~200px~~]]		==STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2==
			<StructureSection load='1mvb' size='340' side='right' caption='[[1mvb]], [[Resolution\|resolution]] 3.00Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MVB FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mvb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1mvb RCSB], [http://www.ebi.ac.uk/pdbsum/1mvb PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/1mvb_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			In MS2 assembly of phage particles results from an interaction between a coat protein dimer and a stem-loop of the RNA genome (the operator hairpin). Amino acid residues Thr45, which is universally conserved among the small RNA phages, and Thr59 are part of the specific RNA binding pocket and interact directly with the RNA; the former through a hydrogen bond, the latter through hydrophobic contacts. The crystal structures of MS2 protein capsids formed by mutants Thr45Ala and Thr59Ser, both with and without the 19 nt wild-type operator hairpin bound, are reported here. The RNA hairpin binds to these mutants in a similar way to its binding to wild-type protein. In a companion paper both mutants are shown to be deficient in RNA binding in an in vivo assay, but in vitro the equilibrium dissociation constant is significantly higher than wild-type for the Thr45Ala mutant. The change in binding affinity of the Thr45Ala mutant is probably a direct consequence of removal of direct hydrogen bonds between the protein and the RNA. The properties of the Thr59Ser mutant are more difficult to explain, but are consistent with a loss of non-polar contact.

	~~{{STRUCTURE_1mvb\| PDB=1mvb \| SCENE= }}~~		Crystal structures of MS2 coat protein mutants in complex with wild-type RNA operator fragments.,van den Worm SH, Stonehouse NJ, Valegard K, Murray JB, Walton C, Fridborg K, Stockley PG, Liljas L Nucleic Acids Res. 1998 Mar 1;26(5):1345-51. PMID:9469847<ref>PMID:9469847</ref>

	~~===STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_9469847}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[1mvb]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:009469847<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Enterobacterio phage ms2]]		[[Category: Enterobacterio phage ms2]]
	[[Category: Liljas, L.]]		[[Category: Liljas, L.]]

OCA: Protected "1mvb" [edit=sysop:move=sysop]

2012-12-05T20:28:39Z

Protected "1mvb" [edit=sysop:move=sysop]

← Older revision	Revision as of 20:28, 5 December 2012
(No difference)

OCA at 20:28, 5 December 2012

2012-12-05T20:28:36Z

← Older revision		Revision as of 20:28, 5 December 2012
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	~~{{Seed}}~~
	[[Image:1mvb.png\|left\|200px]]		[[Image:1mvb.png\|left\|200px]]

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	{{STRUCTURE_1mvb\| PDB=1mvb \| SCENE= }}		{{STRUCTURE_1mvb\| PDB=1mvb \| SCENE= }}

	===STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2===		===STRUCTURE OF A PROTEIN CAPSID OF THE T59S MUTANT OF PHAGE MS2===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_9469847}}, adds the Publication Abstract to the page~~
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	{{ABSTRACT_PUBMED_9469847}}		{{ABSTRACT_PUBMED_9469847}}

	==About this Structure==		==About this Structure==
	~~1MVB~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA].		[[1mvb]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacterio_phage_ms2 Enterobacterio phage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVB OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~9469847~~</ref><references group="xtra"/>		<ref group="xtra">PMID:009469847</ref><references group="xtra"/>
	[[Category: Enterobacterio phage ms2]]		[[Category: Enterobacterio phage ms2]]
	[[Category: Liljas, L.]]		[[Category: Liljas, L.]]
Line 31:		Line 19:
	[[Category: Bacteriophage coat protein]]		[[Category: Bacteriophage coat protein]]
	[[Category: Icosahedral virus]]		[[Category: Icosahedral virus]]
			[[Category: Virus]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 21:50:26 2009''~~