1o7f - Revision history

OCA at 08:57, 9 May 2024

2024-05-09T08:57:48Z

← Older revision		Revision as of 08:57, 9 May 2024
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7f ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Cyclic adenosine monophosphate (cAMP) is a universal second messenger that, in eukaryotes, was believed to act only on cAMP-dependent protein kinase A (PKA) and cyclic nucleotide-regulated ion channels. Recently, guanine nucleotide exchange factors specific for the small GTP-binding proteins Rap1 and Rap2 (Epacs) were described, which are also activated directly by cAMP. Here, we have determined the three-dimensional structure of the regulatory domain of Epac2, which consists of two cyclic nucleotide monophosphate (cNMP)-binding domains and one DEP (Dishevelled, Egl, Pleckstrin) domain. This is the first structure of a cNMP-binding domain in the absence of ligand, and comparison with previous structures, sequence alignment and biochemical experiments allow us to delineate a mechanism for cyclic nucleotide-mediated conformational change and activation that is most likely conserved for all cNMP-regulated proteins. We identify a hinge region that couples cAMP binding to a conformational change of the C-terminal regions. Mutations in the hinge of Epac can uncouple cAMP binding from its exchange activity.

			Structure and regulation of the cAMP-binding domains of Epac2.,Rehmann H, Prakash B, Wolf E, Rueppel A, de Rooij J, Bos JL, Wittinghofer A Nat Struct Biol. 2003 Jan;10(1):26-32. PMID:12469113<ref>PMID:12469113</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 1o7f" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:55, 10 April 2024

2024-04-10T08:55:43Z

← Older revision		Revision as of 08:55, 10 April 2024
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	<StructureSection load='1o7f' size='340' side='right'caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1o7f' size='340' side='right'caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7F FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7F FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [https://pdbe.org/1o7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [https://www.ebi.ac.uk/pdbsum/1o7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7f ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [https://pdbe.org/1o7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [https://www.ebi.ac.uk/pdbsum/1o7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7f ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/RPGF4_MOUSE RPGF4_MOUSE] Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.<ref>PMID:11056535</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7f ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Cyclic adenosine monophosphate (cAMP) is a universal second messenger that, in eukaryotes, was believed to act only on cAMP-dependent protein kinase A (PKA) and cyclic nucleotide-regulated ion channels. Recently, guanine nucleotide exchange factors specific for the small GTP-binding proteins Rap1 and Rap2 (Epacs) were described, which are also activated directly by cAMP. Here, we have determined the three-dimensional structure of the regulatory domain of Epac2, which consists of two cyclic nucleotide monophosphate (cNMP)-binding domains and one DEP (Dishevelled, Egl, Pleckstrin) domain. This is the first structure of a cNMP-binding domain in the absence of ligand, and comparison with previous structures, sequence alignment and biochemical experiments allow us to delineate a mechanism for cyclic nucleotide-mediated conformational change and activation that is most likely conserved for all cNMP-regulated proteins. We identify a hinge region that couples cAMP binding to a conformational change of the C-terminal regions. Mutations in the hinge of Epac can uncouple cAMP binding from its exchange activity.

	Structure and regulation of the cAMP-binding domains of Epac2.,Rehmann H, Prakash B, Wolf E, Rueppel A, de Rooij J, Bos JL, Wittinghofer A Nat Struct Biol. 2003 Jan;10(1):26-32. PMID:12469113<ref>PMID:12469113</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1o7f" style="background-color:#fffaf0;"></div>~~
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Bos~~, J L~~]]		[[Category: Bos JL]]
	[[Category: ~~Prakash, B~~]]		[[Category: De Rooij J]]
	[[Category: ~~Rehmann, H~~]]		[[Category: Prakash B]]
	[[Category: ~~Rooij, J De~~]]		[[Category: Rehmann H]]
	[[Category: Rueppel, A]]		[[Category: Rueppel A]]
	[[Category: Wittinghofer, A]]		[[Category: Wittinghofer A]]
	[[Category: Wolf, E]]		[[Category: Wolf E]]
	~~[[Category: Camp]]~~
	~~[[Category: Camp-gef2]]~~
	~~[[Category: Campb binding doamin]]~~
	~~[[Category: Epac2]]~~
	~~[[Category: Exchange factor]]~~
	~~[[Category: Gef]]~~
	~~[[Category: Regulation~~]]

OCA at 09:55, 12 May 2021

2021-05-12T09:55:59Z

← Older revision		Revision as of 09:55, 12 May 2021
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	<StructureSection load='1o7f' size='340' side='right'caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1o7f' size='340' side='right'caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1O7F FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7F FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [~~http~~://pdbe.org/1o7f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1o7f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7f ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [https://pdbe.org/1o7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [https://www.ebi.ac.uk/pdbsum/1o7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7f ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 06:46, 7 August 2019

2019-08-07T06:46:30Z

← Older revision		Revision as of 06:46, 7 August 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2==		==CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2==
	<StructureSection load='1o7f' size='340' side='right' caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1o7f' size='340' side='right'caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7F FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7F FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7f_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7f_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Bos, J L]]		[[Category: Bos, J L]]

OCA at 09:56, 1 November 2017

2017-11-01T09:56:03Z

← Older revision		Revision as of 09:56, 1 November 2017
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2==		==CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2==
	<StructureSection load='1o7f' size='340' side='right' caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1o7f' size='340' side='right' caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7F FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7F FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [http://pdbe.org/1o7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [http://www.ebi.ac.uk/pdbsum/1o7f PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [http://pdbe.org/1o7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [http://www.ebi.ac.uk/pdbsum/1o7f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7f ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:24, 12 September 2015

2015-09-12T01:24:33Z

← Older revision		Revision as of 01:24, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1o7f' size='340' side='right' caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1o7f' size='340' side='right' caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7F FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7F FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [http://www.ebi.ac.uk/pdbsum/1o7f PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [http://pdbe.org/1o7f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [http://www.ebi.ac.uk/pdbsum/1o7f PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1o7f" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Bos, J L]]		[[Category: Bos, J L]]
	[[Category: Prakash, B]]		[[Category: Prakash, B]]

OCA at 15:30, 5 January 2015

2015-01-05T15:30:29Z

← Older revision		Revision as of 15:30, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7F FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7F FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [http://www.ebi.ac.uk/pdbsum/1o7f PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [http://www.ebi.ac.uk/pdbsum/1o7f PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Bos, J L.]]		[[Category: Bos, J L]]
	[[Category: Prakash, B.]]		[[Category: Prakash, B]]
	[[Category: Rehmann, H.]]		[[Category: Rehmann, H]]
	[[Category: Rooij, J De.]]		[[Category: Rooij, J De]]
	[[Category: Rueppel, A.]]		[[Category: Rueppel, A]]
	[[Category: Wittinghofer, A.]]		[[Category: Wittinghofer, A]]
	[[Category: Wolf, E.]]		[[Category: Wolf, E]]
	[[Category: Camp]]		[[Category: Camp]]
	[[Category: Camp-gef2]]		[[Category: Camp-gef2]]

OCA at 16:39, 28 September 2014

2014-09-28T16:39:35Z

← Older revision		Revision as of 16:39, 28 September 2014
Line 1:		Line 1:
	[[Image:~~1o7f~~.~~png~~\|~~left~~\|~~200px~~]]		==CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2==
			<StructureSection load='1o7f' size='340' side='right' caption='[[1o7f]], [[Resolution\|resolution]] 2.50Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7F FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7f RCSB], [http://www.ebi.ac.uk/pdbsum/1o7f PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7f_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Cyclic adenosine monophosphate (cAMP) is a universal second messenger that, in eukaryotes, was believed to act only on cAMP-dependent protein kinase A (PKA) and cyclic nucleotide-regulated ion channels. Recently, guanine nucleotide exchange factors specific for the small GTP-binding proteins Rap1 and Rap2 (Epacs) were described, which are also activated directly by cAMP. Here, we have determined the three-dimensional structure of the regulatory domain of Epac2, which consists of two cyclic nucleotide monophosphate (cNMP)-binding domains and one DEP (Dishevelled, Egl, Pleckstrin) domain. This is the first structure of a cNMP-binding domain in the absence of ligand, and comparison with previous structures, sequence alignment and biochemical experiments allow us to delineate a mechanism for cyclic nucleotide-mediated conformational change and activation that is most likely conserved for all cNMP-regulated proteins. We identify a hinge region that couples cAMP binding to a conformational change of the C-terminal regions. Mutations in the hinge of Epac can uncouple cAMP binding from its exchange activity.

	~~{{STRUCTURE_1o7f\| PDB=1o7f \| SCENE= }}~~		Structure and regulation of the cAMP-binding domains of Epac2.,Rehmann H, Prakash B, Wolf E, Rueppel A, de Rooij J, Bos JL, Wittinghofer A Nat Struct Biol. 2003 Jan;10(1):26-32. PMID:12469113<ref>PMID:12469113</ref>

	~~===CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_12469113}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[1o7f]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:012469113<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Bos, J L.]]		[[Category: Bos, J L.]]

OCA: Protected "1o7f" [edit=sysop:move=sysop]

2012-12-12T21:53:42Z

Protected "1o7f" [edit=sysop:move=sysop]

← Older revision	Revision as of 21:53, 12 December 2012
(No difference)

OCA at 21:53, 12 December 2012

2012-12-12T21:53:35Z

← Older revision		Revision as of 21:53, 12 December 2012
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	~~{{Seed}}~~
	[[Image:1o7f.png\|left\|200px]]		[[Image:1o7f.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_1o7f", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_1o7f\| PDB=1o7f \| SCENE= }}		{{STRUCTURE_1o7f\| PDB=1o7f \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2===		===CRYSTAL STRUCTURE OF THE REGULATORY DOMAIN OF EPAC2===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_12469113}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 12469113 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_12469113}}		{{ABSTRACT_PUBMED_12469113}}

	==About this Structure==		==About this Structure==
	~~1O7F~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA].		[[1o7f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7F OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~12469113~~</ref><references group="xtra"/>		<ref group="xtra">PMID:012469113</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Bos, J L.]]		[[Category: Bos, J L.]]
Line 39:		Line 27:
	[[Category: Gef]]		[[Category: Gef]]
	[[Category: Regulation]]		[[Category: Regulation]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:07:59 2009''~~