1o7i - Revision history

OCA at 08:57, 9 May 2024

2024-05-09T08:57:50Z

← Older revision		Revision as of 08:57, 9 May 2024
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7i ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7i ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Information processing pathways such as DNA replication are conserved in eukaryotes and archaea and are significantly different from those found in bacteria. Single-stranded DNA-binding (SSB) proteins (or replication protein A, RPA, in eukaryotes) play a central role in many of these pathways. However, whilst euryarchaea have a eukaryotic-type RPA homologue, crenarchaeal SSB proteins appear much more similar to the bacterial proteins, with a single OB fold for DNA binding and a flexible C-terminal tail that is implicated in protein-protein interactions. We have determined the crystal structure of the SSB protein from the crenarchaeote Sulfolobus solfataricus to 1.26 A. The structure shows a striking and unexpected similarity to the DNA-binding domains of human RPA, providing confirmation of the close relationship between archaea and eukaryotes. The high resolution of the structure, together with thermodynamic and mutational studies of DNA binding, allow us to propose a molecular basis for DNA binding and define the features required for eukaryotic and archaeal OB folds.

			Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein.,Kerr ID, Wadsworth RI, Cubeddu L, Blankenfeldt W, Naismith JH, White MF EMBO J. 2003 Jun 2;22(11):2561-70. PMID:12773373<ref>PMID:12773373</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 1o7i" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 08:55, 10 April 2024

2024-04-10T08:55:44Z

← Older revision		Revision as of 08:55, 10 April 2024
Line 3:		Line 3:
	<StructureSection load='1o7i' size='340' side='right'caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='1o7i' size='340' side='right'caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Sacs2 Sacs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1O7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.2Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [~~http~~://pdbe.org/1o7i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [https://pdbe.org/1o7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [https://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/SSB_SACS2 SSB_SACS2] Binds to ssDNA, binding approximately 5 nucleotides per monomer. Binding my be slightly cooperative. Inhibits the endonuclease activity of XPF.<ref>PMID:12675797</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7i ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7i ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Information processing pathways such as DNA replication are conserved in eukaryotes and archaea and are significantly different from those found in bacteria. Single-stranded DNA-binding (SSB) proteins (or replication protein A, RPA, in eukaryotes) play a central role in many of these pathways. However, whilst euryarchaea have a eukaryotic-type RPA homologue, crenarchaeal SSB proteins appear much more similar to the bacterial proteins, with a single OB fold for DNA binding and a flexible C-terminal tail that is implicated in protein-protein interactions. We have determined the crystal structure of the SSB protein from the crenarchaeote Sulfolobus solfataricus to 1.26 A. The structure shows a striking and unexpected similarity to the DNA-binding domains of human RPA, providing confirmation of the close relationship between archaea and eukaryotes. The high resolution of the structure, together with thermodynamic and mutational studies of DNA binding, allow us to propose a molecular basis for DNA binding and define the features required for eukaryotic and archaeal OB folds.

	Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein.,Kerr ID, Wadsworth RI, Cubeddu L, Blankenfeldt W, Naismith JH, White MF EMBO J. 2003 Jun 2;22(11):2561-70. PMID:12773373<ref>PMID:12773373</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1o7i" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Sacs2~~]]		[[Category: Saccharolobus solfataricus P2]]
	[[Category: Kerr~~, I D~~]]		[[Category: Kerr ID]]
	[[Category: Naismith~~, J H]]~~		[[Category: Naismith JH]]
	~~[[Category: Dna binding protein]]~~
	~~[[Category: Dna-binding protein]]~~
	~~[[Category: Ob fold]]~~
	~~[[Category: Single stranded dna~~]]

OCA at 09:04, 2 December 2020

2020-12-02T09:04:56Z

← Older revision		Revision as of 09:04, 2 December 2020
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	==Crystal structure of a single stranded DNA binding protein==		==Crystal structure of a single stranded DNA binding protein==
	<StructureSection load='1o7i' size='340' side='right' caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='1o7i' size='340' side='right'caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1O7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://pdbe.org/1o7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://pdbe.org/1o7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 28:		Line 28:

	==See Also==		==See Also==
	*[[Single-stranded DNA-binding protein\|Single-stranded DNA-binding protein]]		*[[Single-stranded DNA-binding protein 3D structures\|Single-stranded DNA-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Sacs2]]		[[Category: Sacs2]]
	[[Category: Kerr, I D]]		[[Category: Kerr, I D]]

OCA at 08:47, 30 January 2019

2019-01-30T08:47:45Z

← Older revision		Revision as of 08:47, 30 January 2019
Line 3:		Line 3:
	<StructureSection load='1o7i' size='340' side='right' caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='1o7i' size='340' side='right' caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulso Sulso~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://pdbe.org/1o7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://pdbe.org/1o7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>
Line 26:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1o7i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1o7i" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Single-stranded DNA-binding protein\|Single-stranded DNA-binding protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Sulso~~]]		[[Category: Sacs2]]
	[[Category: Kerr, I D]]		[[Category: Kerr, I D]]
	[[Category: Naismith, J H]]		[[Category: Naismith, J H]]

OCA at 07:15, 7 February 2018

2018-02-07T07:15:55Z

← Older revision		Revision as of 07:15, 7 February 2018
Line 1:		Line 1:
	==~~CRYSTAL STRUCTURE OF A SINGLE STRANDED~~ DNA ~~BINDING PROTEIN~~==
			==Crystal structure of a single stranded DNA binding protein==
	<StructureSection load='1o7i' size='340' side='right' caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='1o7i' size='340' side='right' caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://pdbe.org/1o7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://pdbe.org/1o7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7i ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7i_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7i_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 25:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1o7i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1o7i" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Single-stranded DNA-binding protein\|Single-stranded DNA-binding protein]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 33:
	[[Category: Kerr, I D]]		[[Category: Kerr, I D]]
	[[Category: Naismith, J H]]		[[Category: Naismith, J H]]
			[[Category: Dna binding protein]]
	[[Category: Dna-binding protein]]		[[Category: Dna-binding protein]]
	[[Category: Ob fold]]		[[Category: Ob fold]]
	[[Category: Single stranded dna]]		[[Category: Single stranded dna]]

OCA at 23:30, 8 February 2016

2016-02-08T23:30:39Z

← Older revision		Revision as of 23:30, 8 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7i ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:24, 10 September 2015

2015-09-10T16:24:51Z

← Older revision		Revision as of 16:24, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1o7i' size='340' side='right' caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='1o7i' size='340' side='right' caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://pdbe.org/1o7i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1o7i" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Sulfolobus solfataricus p2~~]]		[[Category: Sulso]]
	[[Category: Kerr, I D]]		[[Category: Kerr, I D]]
	[[Category: Naismith, J H]]		[[Category: Naismith, J H]]

OCA at 15:43, 5 January 2015

2015-01-05T15:43:24Z

← Older revision		Revision as of 15:43, 5 January 2015
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Sulfolobus solfataricus p2]]		[[Category: Sulfolobus solfataricus p2]]
	[[Category: Kerr, I D.]]		[[Category: Kerr, I D]]
	[[Category: Naismith, J H.]]		[[Category: Naismith, J H]]
	[[Category: Dna-binding protein]]		[[Category: Dna-binding protein]]
	[[Category: Ob fold]]		[[Category: Ob fold]]
	[[Category: Single stranded dna]]		[[Category: Single stranded dna]]

OCA at 14:03, 29 September 2014

2014-09-29T14:03:59Z

← Older revision		Revision as of 14:03, 29 September 2014
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	[[Image:~~1o7i~~.~~png~~\|~~left~~\|~~200px~~]]		==CRYSTAL STRUCTURE OF A SINGLE STRANDED DNA BINDING PROTEIN==
			<StructureSection load='1o7i' size='340' side='right' caption='[[1o7i]], [[Resolution\|resolution]] 1.20Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1o7i]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7I FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7i RCSB], [http://www.ebi.ac.uk/pdbsum/1o7i PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7i_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Information processing pathways such as DNA replication are conserved in eukaryotes and archaea and are significantly different from those found in bacteria. Single-stranded DNA-binding (SSB) proteins (or replication protein A, RPA, in eukaryotes) play a central role in many of these pathways. However, whilst euryarchaea have a eukaryotic-type RPA homologue, crenarchaeal SSB proteins appear much more similar to the bacterial proteins, with a single OB fold for DNA binding and a flexible C-terminal tail that is implicated in protein-protein interactions. We have determined the crystal structure of the SSB protein from the crenarchaeote Sulfolobus solfataricus to 1.26 A. The structure shows a striking and unexpected similarity to the DNA-binding domains of human RPA, providing confirmation of the close relationship between archaea and eukaryotes. The high resolution of the structure, together with thermodynamic and mutational studies of DNA binding, allow us to propose a molecular basis for DNA binding and define the features required for eukaryotic and archaeal OB folds.

	~~{{STRUCTURE_1o7i\| PDB=1o7i \| SCENE= }}~~		Insights into ssDNA recognition by the OB fold from a structural and thermodynamic study of Sulfolobus SSB protein.,Kerr ID, Wadsworth RI, Cubeddu L, Blankenfeldt W, Naismith JH, White MF EMBO J. 2003 Jun 2;22(11):2561-70. PMID:12773373<ref>PMID:12773373</ref>

	~~===CRYSTAL STRUCTURE OF A SINGLE STRANDED DNA BINDING PROTEIN===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_12773373}}~~		==See Also==
			*[[Single-stranded DNA-binding protein\|Single-stranded DNA-binding protein]]
	==~~About this Structure~~==		== References ==
	[[~~1o7i~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7I OCA].		<references/>
			__TOC__
	==~~Reference~~==		</StructureSection>
	<~~ref group="xtra">PMID:012773373<~~/~~ref~~><~~ref group="xtra">PMID:011526322</ref><references group="xtra"~~/>		[[Category: Sulfolobus solfataricus p2]]
	[[Category: Sulfolobus solfataricus]]
	[[Category: Kerr, I D.]]		[[Category: Kerr, I D.]]
	[[Category: Naismith, J H.]]		[[Category: Naismith, J H.]]

OCA at 09:51, 16 January 2013

2013-01-16T09:51:03Z

← Older revision		Revision as of 09:51, 16 January 2013
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	[[Image:1o7i.png\|left\|200px]]		[[Image:1o7i.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_1o7i", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_1o7i\| PDB=1o7i \| SCENE= }}		{{STRUCTURE_1o7i\| PDB=1o7i \| SCENE= }}

	===CRYSTAL STRUCTURE OF A SINGLE STRANDED DNA BINDING PROTEIN===		===CRYSTAL STRUCTURE OF A SINGLE STRANDED DNA BINDING PROTEIN===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_12773373}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 12773373 is the PubMed ID number.~~
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	{{ABSTRACT_PUBMED_12773373}}		{{ABSTRACT_PUBMED_12773373}}

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	==Reference==		==Reference==
	<ref group="xtra">PMID:~~12773373~~</ref><ref group="xtra">PMID:~~11526322~~</ref><references group="xtra"/>		<ref group="xtra">PMID:012773373</ref><ref group="xtra">PMID:011526322</ref><references group="xtra"/>
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Kerr, I D.]]		[[Category: Kerr, I D.]]