1o7o - Revision history

OCA at 12:29, 13 December 2023

2023-12-13T12:29:40Z

← Older revision		Revision as of 12:29, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='1o7o' size='340' side='right'caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='1o7o' size='340' side='right'caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7O FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BGC:BETA~~-~~D-GLUCOSE'>BGC</scene>~~, ~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.97Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]~~</~~div~~><~~/td~~></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<~~tr id~~='~~activity~~'><~~td class="sblockLbl"~~><~~b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/N~~-~~acetyllactosaminide_3~~-~~alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [https://pdbe.org/1o7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [https://www.ebi.ac.uk/pdbsum/1o7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [https://pdbe.org/1o7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [https://www.ebi.ac.uk/pdbsum/1o7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7o ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R).		[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bovin~~]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]~~		[[Category: Acharya KR]]
	[[Category: Acharya~~, K R~~]]		[[Category: Brew K]]
	[[Category: Brew, K]]		[[Category: Deshpande A]]
	[[Category: Deshpande, A]]		[[Category: Natesh R]]
	[[Category: Natesh, R]]		[[Category: Swaminathan GJ]]
	[[Category: Swaminathan~~, G J~~]]		[[Category: Xie Z]]
	[[Category: Xie, Z]]		[[Category: Zhang Y]]
	[[Category: Zhang, Y]]
	~~[[Category: Glycoprotein]]~~
	~~[[Category: Glycosyltransferase]]~~
	~~[[Category: Transferase~~]]

OCA at 09:17, 8 September 2021

2021-09-08T09:17:55Z

← Older revision		Revision as of 09:17, 8 September 2021
Line 3:		Line 3:
	<StructureSection load='1o7o' size='340' side='right'caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='1o7o' size='340' side='right'caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1O7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O7O FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~LAT~~:BETA-~~LACTOSE~~'>~~LAT~~</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</div></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [~~http~~://pdbe.org/1o7o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1o7o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [https://pdbe.org/1o7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [https://www.ebi.ac.uk/pdbsum/1o7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7o ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R).		[[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:30, 4 December 2019

2019-12-04T11:30:56Z

← Older revision		Revision as of 11:30, 4 December 2019
Line 1:		Line 1:

	==Roles of Individual Residues of Alpha-1,3 Galactosyltransferases in Substrate Binding and Catalysis==		==Roles of Individual Residues of Alpha-1,3 Galactosyltransferases in Substrate Binding and Catalysis==
	<StructureSection load='1o7o' size='340' side='right' caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='1o7o' size='340' side='right'caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7O FirstGlance]. <br>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1o7o" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1o7o" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Bovin]]		[[Category: Bovin]]
			[[Category: Large Structures]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]

OCA at 07:20, 7 February 2018

2018-02-07T07:20:21Z

← Older revision		Revision as of 07:20, 7 February 2018
Line 1:		Line 1:

	==Roles of Individual Residues of Alpha-1,3 Galactosyltransferases in Substrate Binding and Catalysis==		==Roles of Individual Residues of Alpha-1,3 Galactosyltransferases in Substrate Binding and Catalysis==
	<StructureSection load='1o7o' size='340' side='right' caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='1o7o' size='340' side='right' caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [http://pdbe.org/1o7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [http://www.ebi.ac.uk/pdbsum/1o7o PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [http://pdbe.org/1o7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [http://www.ebi.ac.uk/pdbsum/1o7o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1o7o ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7o_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7o_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1o7o" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1o7o" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Glycosyltransferase\|Glycosyltransferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:39, 8 February 2016

2016-02-08T09:39:17Z

← Older revision		Revision as of 09:39, 8 February 2016
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [http://www.ebi.ac.uk/pdbsum/1o7o PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [http://pdbe.org/1o7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [http://www.ebi.ac.uk/pdbsum/1o7o PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o7o ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1o7o" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 06:19, 15 April 2015

2015-04-15T06:19:53Z

← Older revision		Revision as of 06:19, 15 April 2015
Line 1:		Line 1:
	==~~ROLES OF INDIVIDUAL RESIDUES OF ALPHA~~-1,3 ~~GALACTOSYLTRANSFERASES IN SUBSTRATE BINDING AND CATALYSIS~~==		==Roles of Individual Residues of Alpha-1,3 Galactosyltransferases in Substrate Binding and Catalysis==
	<StructureSection load='1o7o' size='340' side='right' caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='1o7o' size='340' side='right' caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7O FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>
Line 35:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bos taurus~~]]		[[Category: Bovin]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]
Line 42:		Line 42:
	[[Category: Natesh, R]]		[[Category: Natesh, R]]
	[[Category: Swaminathan, G J]]		[[Category: Swaminathan, G J]]
	[[Category: Xie, X]]		[[Category: Xie, Z]]
	[[Category: Zhang, Y]]		[[Category: Zhang, Y]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 22:22, 24 December 2014

2014-12-24T22:22:56Z

← Older revision		Revision as of 22:22, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7O FirstGlance]. <br>		<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7O FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [http://www.ebi.ac.uk/pdbsum/1o7o PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [http://www.ebi.ac.uk/pdbsum/1o7o PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]
	[[Category: Acharya, K R.]]		[[Category: Acharya, K R]]
	[[Category: Brew, K.]]		[[Category: Brew, K]]
	[[Category: Deshpande, A.]]		[[Category: Deshpande, A]]
	[[Category: Natesh, R.]]		[[Category: Natesh, R]]
	[[Category: Swaminathan, G J.]]		[[Category: Swaminathan, G J]]
	[[Category: Xie, X.]]		[[Category: Xie, X]]
	[[Category: Zhang, Y.]]		[[Category: Zhang, Y]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 13:20, 28 September 2014

2014-09-28T13:20:59Z

← Older revision		Revision as of 13:20, 28 September 2014
Line 1:		Line 1:
	[[Image:~~1o7o~~.~~png~~\|~~left~~\|~~200px~~]]		==ROLES OF INDIVIDUAL RESIDUES OF ALPHA-1,3 GALACTOSYLTRANSFERASES IN SUBSTRATE BINDING AND CATALYSIS==
			<StructureSection load='1o7o' size='340' side='right' caption='[[1o7o]], [[Resolution\|resolution]] 1.97Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O7O FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7q\|1o7q]], [[1o7r\|1o7r]]</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o7o RCSB], [http://www.ebi.ac.uk/pdbsum/1o7o PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o7/1o7o_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The retaining glycosyltransferase, alpha-1,3-galactosyltransferase (alpha3GT), is mutationally inactivated in humans, leading to the presence of circulating antibodies against its product, the alpha-Gal epitope. alpha3GT catalyzes galactose transfer from UDP-Gal to beta-linked galactosides, such as lactose, and in the absence of an acceptor substrate, to water at a lower rate. We have used site-directed mutagenesis to investigate the roles in catalysis and specificity of residues in alpha3GT that form H-bonds as well as other interactions with substrates. Mutation of the conserved Glu(317) to Gln weakens lactose binding and reduces the k(cat) for galactosyltransfer to lactose and water by 2400 and 120, respectively. The structure is not perturbed by this substitution, but the orientation of the bound lactose molecule is changed. The magnitude of these changes does not support a previous proposal that Glu(317) is the catalytic nucleophile in a double displacement mechanism and suggests it acts in acceptor substrate binding and in stabilizing a cationic transition state for cleavage of the bond between UDP and C1 of the galactose. Cleavage of this bond also linked to a conformational change in the C-terminal region of alpha3GT that is coupled with UDP binding. Mutagenesis indicates that His(280), which is projected to interact with the 2-OH of the galactose moiety of UDP-Gal, is a key residue in the stringent donor substrate specificity through its role in stabilizing the bound UDP-Gal in a suitable conformation for catalysis. Mutation of Gln(247), which forms multiple interactions with acceptor substrates, to Glu reduces the catalytic rate of galactose transfer to lactose but not to water. This mutation is predicted to perturb the orientation or environment of the bound acceptor substrate. The results highlight the importance of H-bonds between enzyme and substrates in this glycosyltransferase, in arranging substrates in appropriate conformations and orientation for efficient catalysis. These factors are manifested in increases in catalytic rate rather than substrate affinity.

	~~{{STRUCTURE_1o7o\| PDB=1o7o \| SCENE= }}~~		Roles of individual enzyme-substrate interactions by alpha-1,3-galactosyltransferase in catalysis and specificity.,Zhang Y, Swaminathan GJ, Deshpande A, Boix E, Natesh R, Xie Z, Acharya KR, Brew K Biochemistry. 2003 Nov 25;42(46):13512-21. PMID:14621997<ref>PMID:14621997</ref>

	~~===ROLES OF INDIVIDUAL RESIDUES OF ALPHA-1~~,~~3 GALACTOSYLTRANSFERASES IN SUBSTRATE BINDING AND CATALYSIS===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_14621997}}~~

	~~==About this Structure==~~
	~~[[1o7o]] is~~ a ~~2 chain structure with sequence from [http://en.wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann.ac.il/oca-bin~~/~~ocashort?id=1O7O OCA].~~

	==See Also==		==See Also==
	*[[~~Galactosyltransferase~~\|~~Galactosyltransferase~~]]		*[[Glycosyltransferase\|Glycosyltransferase]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:014621997<~~/~~ref~~><~~ref group="xtra">PMID:011179209</ref><ref group="xtra">PMID:011592969</ref><references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]

OCA at 09:44, 20 October 2012

2012-10-20T09:44:04Z

← Older revision		Revision as of 09:44, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[1o7o]] is a 2 chain structure ~~of [[Galactosyltransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA].		[[1o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA].

	==See Also==		==See Also==

OCA at 23:47, 25 July 2012

2012-07-25T23:47:36Z

← Older revision		Revision as of 23:47, 25 July 2012
Line 1:		Line 1:
	[[Image:1o7o.png\|left\|200px]]		[[Image:1o7o.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_1o7o", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_1o7o\| PDB=1o7o \| SCENE= }}		{{STRUCTURE_1o7o\| PDB=1o7o \| SCENE= }}

	===ROLES OF INDIVIDUAL RESIDUES OF ALPHA-1,3 GALACTOSYLTRANSFERASES IN SUBSTRATE BINDING AND CATALYSIS===		===ROLES OF INDIVIDUAL RESIDUES OF ALPHA-1,3 GALACTOSYLTRANSFERASES IN SUBSTRATE BINDING AND CATALYSIS===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_14621997}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 14621997 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_14621997}}		{{ABSTRACT_PUBMED_14621997}}

	==About this Structure==		==About this Structure==
	[[1o7o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA].		[[1o7o]] is a 2 chain structure of [[Galactosyltransferase]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7O OCA].

			==See Also==
			*[[Galactosyltransferase\|Galactosyltransferase]]

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~14621997~~</ref><ref group="xtra">PMID:~~11179209~~</ref><ref group="xtra">PMID:~~11592969~~</ref><references group="xtra"/>		<ref group="xtra">PMID:014621997</ref><ref group="xtra">PMID:011179209</ref><ref group="xtra">PMID:011592969</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]