1oln - Revision history

OCA at 08:21, 9 April 2025

2025-04-09T08:21:24Z

← Older revision		Revision as of 08:21, 9 April 2025
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OLN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OLN FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solution NMR , Theoretical Model</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solution NMR , Theoretical Model, 1 model</td></tr>
	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [https://pdbe.org/1oln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [https://www.ebi.ac.uk/pdbsum/1oln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [https://pdbe.org/1oln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [https://www.ebi.ac.uk/pdbsum/1oln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr>
Line 15:		Line 15:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/1oln_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/1oln_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oln ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oln ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Thiostrepton and micrococcin inhibit protein synthesis by binding to the L11 binding domain (L11BD) of 23S ribosomal RNA. The two compounds are structurally related, yet they produce different effects on ribosomal RNA in footprinting experiments and on elongation factor-G (EF-G)-dependent GTP hydrolysis. Using NMR and an assay based on A1067 methylation by thiostrepton-resistance methyltransferase, we show that the related thiazoles, nosiheptide and siomycin, also bind to this region. The effect of all four antibiotics on EF-G-dependent GTP hydrolysis and EF-G-GDP-ribosome complex formation was studied. Our NMR and biochemical data demonstrate that thiostrepton, nosiheptide, and siomycin share a common profile, which differs from that of micrococcin. We have generated a three-dimensional (3D) model for the interaction of thiostrepton with L11BD RNA. The model rationalizes the differences between micrococcin and the thiostrepton-like antibiotics interacting with L11BD.

			Structural basis for contrasting activities of ribosome binding thiazole antibiotics.,Lentzen G, Klinck R, Matassova N, Aboul-ela F, Murchie AI Chem Biol. 2003 Aug;10(8):769-78. PMID:12954336<ref>PMID:12954336</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 1oln" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribosomal protein L11 3D structures\|Ribosomal protein L11 3D structures]]		*[[Ribosomal protein L11 3D structures\|Ribosomal protein L11 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:48, 17 April 2024

2024-04-17T05:48:56Z

← Older revision		Revision as of 05:48, 17 April 2024
Line 1:		Line 1:

	==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA==		==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA==
	<StructureSection load='1oln' size='340' side='right'caption='[[1oln~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>		<StructureSection load='1oln' size='340' side='right'caption='[[1oln]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OLN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OLN FirstGlance]. <br>
	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solution NMR , Theoretical Model</td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</div></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [https://pdbe.org/1oln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [https://www.ebi.ac.uk/pdbsum/1oln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [https://pdbe.org/1oln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [https://www.ebi.ac.uk/pdbsum/1oln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA]] This protein binds directly to 23S ribosomal RNA~~. [[https://www.uniprot.org/uniprot/THCL_STRAJ THCL_STRAJ]] Has bacteriocidal activity. Inhibits bacterial protein biosynthesis by acting on the elongation factor Tu (EF-Tu) (By similarity)~~.		[https://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA] This protein binds directly to 23S ribosomal RNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oln ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oln ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Thiostrepton and micrococcin inhibit protein synthesis by binding to the L11 binding domain (L11BD) of 23S ribosomal RNA. The two compounds are structurally related, yet they produce different effects on ribosomal RNA in footprinting experiments and on elongation factor-G (EF-G)-dependent GTP hydrolysis. Using NMR and an assay based on A1067 methylation by thiostrepton-resistance methyltransferase, we show that the related thiazoles, nosiheptide and siomycin, also bind to this region. The effect of all four antibiotics on EF-G-dependent GTP hydrolysis and EF-G-GDP-ribosome complex formation was studied. Our NMR and biochemical data demonstrate that thiostrepton, nosiheptide, and siomycin share a common profile, which differs from that of micrococcin. We have generated a three-dimensional (3D) model for the interaction of thiostrepton with L11BD RNA. The model rationalizes the differences between micrococcin and the thiostrepton-like antibiotics interacting with L11BD.

	Structural basis for contrasting activities of ribosome binding thiazole antibiotics.,Lentzen G, Klinck R, Matassova N, Aboul-ela F, Murchie AI Chem Biol. 2003 Aug;10(8):769-78. PMID:12954336<ref>PMID:12954336</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1oln" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Ribosomal protein L11\|Ribosomal protein L11]]		*[[Ribosomal protein L11 3D structures\|Ribosomal protein L11 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
Line 39:		Line 28:
	[[Category: Streptomyces azureus]]		[[Category: Streptomyces azureus]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: Aboul-Ela, F]]		[[Category: Aboul-Ela F]]
	[[Category: Klinck, R]]		[[Category: Klinck R]]
	[[Category: Lentzen, G]]		[[Category: Lentzen G]]
	[[Category: Matassova, N]]		[[Category: Matassova N]]
	[[Category: Murchie~~, A I.H]]~~		[[Category: Murchie AIH]]
	~~[[Category: Antibacterial]]~~
	~~[[Category: L11]]~~
	~~[[Category: Oxazole]]~~
	~~[[Category: Ribosome]]~~
	~~[[Category: Ribosome-antibiotic complex]]~~
	~~[[Category: Thiazole]]~~
	~~[[Category: Thiopeptide]]~~
	~~[[Category: Translation inhibition~~]]

OCA at 09:54, 26 May 2021

2021-05-26T09:54:16Z

← Older revision		Revision as of 09:54, 26 May 2021
Line 3:		Line 3:
	<StructureSection load='1oln' size='340' side='right'caption='[[1oln]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1oln' size='340' side='right'caption='[[1oln]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [~~http~~://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1OLN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OLN FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [~~http~~://pdbe.org/1oln PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1oln PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [https://pdbe.org/1oln PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [https://www.ebi.ac.uk/pdbsum/1oln PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA]] This protein binds directly to 23S ribosomal RNA. [[~~http~~://www.uniprot.org/uniprot/THCL_STRAJ THCL_STRAJ]] Has bacteriocidal activity. Inhibits bacterial protein biosynthesis by acting on the elongation factor Tu (EF-Tu) (By similarity).		[[https://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA]] This protein binds directly to 23S ribosomal RNA. [[https://www.uniprot.org/uniprot/THCL_STRAJ THCL_STRAJ]] Has bacteriocidal activity. Inhibits bacterial protein biosynthesis by acting on the elongation factor Tu (EF-Tu) (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:17, 24 April 2019

2019-04-24T07:17:24Z

← Older revision		Revision as of 07:17, 24 April 2019
Line 1:		Line 1:

	==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA==		==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA==
	<StructureSection load='1oln' size='340' side='right' caption='[[1oln]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1oln' size='340' side='right'caption='[[1oln]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OLN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OLN FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/1oln_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/1oln_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1oln" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1oln" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Ribosomal protein L11\|Ribosomal protein L11]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Streptomyces azureus]]		[[Category: Streptomyces azureus]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]

OCA at 07:44, 15 November 2017

2017-11-15T07:44:34Z

← Older revision		Revision as of 07:44, 15 November 2017
Line 1:		Line 1:

	==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA==		==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA==
	<StructureSection load='1oln' size='340' side='right' caption='[[1oln]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1oln' size='340' side='right' caption='[[1oln]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OLN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OLN FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqs\|1jqs]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [http://pdbe.org/1oln PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [http://www.ebi.ac.uk/pdbsum/1oln PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [http://pdbe.org/1oln PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [http://www.ebi.ac.uk/pdbsum/1oln PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1oln ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1oln" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1oln" style="background-color:#fffaf0;"></div>

	~~==See Also==~~
	*[[Ribosomal protein L11\|Ribosomal protein L11]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:41, 8 February 2016

2016-02-08T13:41:45Z

← Older revision		Revision as of 13:41, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oln ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:06, 10 September 2015

2015-09-10T12:06:55Z

← Older revision		Revision as of 12:06, 10 September 2015
Line 5:		Line 5:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [http://www.ebi.ac.uk/pdbsum/1oln PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [http://pdbe.org/1oln PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [http://www.ebi.ac.uk/pdbsum/1oln PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1oln" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Ribosomal protein L11\|Ribosomal protein L11]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:44, 24 December 2014

2014-12-24T23:44:01Z

← Older revision		Revision as of 23:44, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OLN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OLN FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [http://www.ebi.ac.uk/pdbsum/1oln PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [http://www.ebi.ac.uk/pdbsum/1oln PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/RL11_THEMA RL11_THEMA]] This protein binds directly to 23S ribosomal RNA. [[http://www.uniprot.org/uniprot/THCL_STRAJ THCL_STRAJ]] Has bacteriocidal activity. Inhibits bacterial protein biosynthesis by acting on the elongation factor Tu (EF-Tu) (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

	~~==See Also==~~
	*[[Ribosomal protein L11\|Ribosomal protein L11]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 33:
	[[Category: Streptomyces azureus]]		[[Category: Streptomyces azureus]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: Aboul-Ela, F.]]		[[Category: Aboul-Ela, F]]
	[[Category: Klinck, R.]]		[[Category: Klinck, R]]
	[[Category: Lentzen, G.]]		[[Category: Lentzen, G]]
	[[Category: Matassova, N.]]		[[Category: Matassova, N]]
	[[Category: Murchie, A I.H.]]		[[Category: Murchie, A I.H]]
	[[Category: Antibacterial]]		[[Category: Antibacterial]]
	[[Category: L11]]		[[Category: L11]]

OCA at 14:38, 28 September 2014

2014-09-28T14:38:17Z

← Older revision		Revision as of 14:38, 28 September 2014
Line 1:		Line 1:
	[[Image:~~1oln~~.~~png~~\|~~left~~\|~~200px~~]]		==Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA==
			<StructureSection load='1oln' size='340' side='right' caption='[[1oln]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1oln]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1OLN FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BB9:(2Z)-2-AMINO-3-SULFANYLPROP-2-ENOIC+ACID'>BB9</scene>, <scene name='pdbligand=DBU:(2Z)-2-AMINOBUT-2-ENOIC+ACID'>DBU</scene>, <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene>, <scene name='pdbligand=DHA:2-AMINO-ACRYLIC+ACID'>DHA</scene>, <scene name='pdbligand=MH6:3-HYDROXY-2-IMINOPROPANOIC+ACID'>MH6</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=QUA:8-HYDROXY-4-(1-HYDROXYETHYL)QUINOLINE-2-CARBOXYLIC+ACID'>QUA</scene>, <scene name='pdbligand=TS9:(2S,3S,4R)-2-AMINO-3,4-DIHYDROXY-3-METHYLPENTANOIC+ACID'>TS9</scene></td></tr>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d8t\|1d8t]], [[1e9w\|1e9w]], [[2c77\|2c77]], [[2jq7\|2jq7]], [[2zjp\|2zjp]], [[3cf5\|3cf5]], [[1mms\|1mms]], [[1eg0\|1eg0]], [[1giy\|1giy]], [[1jqm\|1jqm]], [[1jqt\|1jqt]], [[1pn7\|1pn7]], [[1pn8\|1pn8]], [[487d\|487d]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oln OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1oln RCSB], [http://www.ebi.ac.uk/pdbsum/1oln PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ol/1oln_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Thiostrepton and micrococcin inhibit protein synthesis by binding to the L11 binding domain (L11BD) of 23S ribosomal RNA. The two compounds are structurally related, yet they produce different effects on ribosomal RNA in footprinting experiments and on elongation factor-G (EF-G)-dependent GTP hydrolysis. Using NMR and an assay based on A1067 methylation by thiostrepton-resistance methyltransferase, we show that the related thiazoles, nosiheptide and siomycin, also bind to this region. The effect of all four antibiotics on EF-G-dependent GTP hydrolysis and EF-G-GDP-ribosome complex formation was studied. Our NMR and biochemical data demonstrate that thiostrepton, nosiheptide, and siomycin share a common profile, which differs from that of micrococcin. We have generated a three-dimensional (3D) model for the interaction of thiostrepton with L11BD RNA. The model rationalizes the differences between micrococcin and the thiostrepton-like antibiotics interacting with L11BD.

	~~{{STRUCTURE_1oln\| PDB=1oln \| SCENE= }}~~		Structural basis for contrasting activities of ribosome binding thiazole antibiotics.,Lentzen G, Klinck R, Matassova N, Aboul-ela F, Murchie AI Chem Biol. 2003 Aug;10(8):769-78. PMID:12954336<ref>PMID:12954336</ref>

	~~===Model for thiostrepton antibiotic binding to L11 substrate from 50S ribosomal RNA===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_12954336}}~~

	~~==About this Structure==~~
	~~[[1oln]] is~~ a ~~3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en~~.~~wikipedia~~.~~org/wiki/Thermotoga_maritima Thermotoga maritima]~~. ~~Full experimental information is available from [http://oca.weizmann.ac.il~~/~~oca-bin/ocashort?id=1OLN OCA].~~

	==See Also==		==See Also==
	*[[Ribosomal protein L11\|Ribosomal protein L11]]		*[[Ribosomal protein L11\|Ribosomal protein L11]]
			== References ==
	==~~Reference~~==		<references/>
	<~~ref group="xtra">PMID:012954336<~~/~~ref~~><~~references group="xtra"~~/>		__TOC__
			</StructureSection>
	[[Category: Streptomyces azureus]]		[[Category: Streptomyces azureus]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]

OCA at 10:20, 20 October 2012

2012-10-20T10:20:39Z

← Older revision		Revision as of 10:20, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
	[[1oln]] is a 3 chain structure ~~of [[Ribosomal protein L11]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA].		[[1oln]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_azureus Streptomyces azureus] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OLN OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
	<ref group="xtra">PMID:012954336~~</ref><ref group="xtra">PMID:010338213</ref><ref group="xtra">PMID:010325228~~</ref><references group="xtra"/>		<ref group="xtra">PMID:012954336</ref><references group="xtra"/>
	[[Category: Streptomyces azureus]]		[[Category: Streptomyces azureus]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]