1tle - Revision history

OCA at 07:28, 30 October 2024

2024-10-30T07:28:37Z

← Older revision		Revision as of 07:28, 30 October 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLE FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 14 models</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [https://pdbe.org/1tle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [https://www.ebi.ac.uk/pdbsum/1tle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tle ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [https://pdbe.org/1tle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [https://www.ebi.ac.uk/pdbsum/1tle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tle ProSAT]</span></td></tr>
	</table>		</table>
Line 14:		Line 14:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tle_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tle_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tle ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tle ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The structure of the single LE module between residues 791 and 848 of the laminin gamma1 chain, which contains the high affinity binding site for nidogen, has been probed using NMR methods. The module folds into an autonomous domain which has a stable and unique three-dimensional (3D) structure in solution. The 3D structure was determined on the basis of 362 interproton distance constraints derived from nuclear Overhauser enhancement measurements and 39 phi angles, supplemented by 5 psi and 22 chi1 angles. The main features of the NMR structures are two-stranded antiparallel beta-sheets which are separated by loops and cross-connected by four disulfide bridges. The N-terminal segment which contains the first three disulfide bridges is similar to epidermal growth factor. The C-terminal segment has an S-like backbone profile with a crossover at the last disulfide bridge and comprises two three-residue long beta-strands that form an antiparallel beta-sheet. The LE module possesses an exposed nidogen binding loop that projects away from the main body of the protein. The side-chains of three amino acids which are crucial for binding (Asp, Asn, Val) are all exposed at the domain surface. An inactivating Asn-Ser mutation in this region showed the same 3D structure indicating that these three residues, and possibly an additional Tyr in an adjacent loop, provide direct contacts in the interaction with nidogen.

			Structure of the nidogen binding LE module of the laminin gamma1 chain in solution.,Baumgartner R, Czisch M, Mayer U, Poschl E, Huber R, Timpl R, Holak TA J Mol Biol. 1996 Apr 5;257(3):658-68. PMID:8648631<ref>PMID:8648631</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 1tle" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Laminin\|Laminin]]		*[[Laminin\|Laminin]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:42, 1 May 2024

2024-05-01T08:42:21Z

← Older revision		Revision as of 08:42, 1 May 2024
Line 1:		Line 1:

	==LE (LAMININ-TYPE EGF-LIKE) MODULE GIII4 IN SOLUTION AT PH 3.5 AND 290 K, NMR, 14 STRUCTURES==		==LE (LAMININ-TYPE EGF-LIKE) MODULE GIII4 IN SOLUTION AT PH 3.5 AND 290 K, NMR, 14 STRUCTURES==
	<StructureSection load='1tle' size='340' side='right'caption='[[1tle~~]], [[NMR_Ensembles_of_Models \| 14 NMR models~~]]' scene=''>		<StructureSection load='1tle' size='340' side='right'caption='[[1tle]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLE FirstGlance]. <br>
	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>LAMC1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10090 LK3 transgenic mice])~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [https://pdbe.org/1tle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [https://www.ebi.ac.uk/pdbsum/1tle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tle ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [https://pdbe.org/1tle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [https://www.ebi.ac.uk/pdbsum/1tle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tle ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/LAMC1_MOUSE LAMC1_MOUSE]] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.		[https://www.uniprot.org/uniprot/LAMC1_MOUSE LAMC1_MOUSE] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tle ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tle ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The structure of the single LE module between residues 791 and 848 of the laminin gamma1 chain, which contains the high affinity binding site for nidogen, has been probed using NMR methods. The module folds into an autonomous domain which has a stable and unique three-dimensional (3D) structure in solution. The 3D structure was determined on the basis of 362 interproton distance constraints derived from nuclear Overhauser enhancement measurements and 39 phi angles, supplemented by 5 psi and 22 chi1 angles. The main features of the NMR structures are two-stranded antiparallel beta-sheets which are separated by loops and cross-connected by four disulfide bridges. The N-terminal segment which contains the first three disulfide bridges is similar to epidermal growth factor. The C-terminal segment has an S-like backbone profile with a crossover at the last disulfide bridge and comprises two three-residue long beta-strands that form an antiparallel beta-sheet. The LE module possesses an exposed nidogen binding loop that projects away from the main body of the protein. The side-chains of three amino acids which are crucial for binding (Asp, Asn, Val) are all exposed at the domain surface. An inactivating Asn-Ser mutation in this region showed the same 3D structure indicating that these three residues, and possibly an additional Tyr in an adjacent loop, provide direct contacts in the interaction with nidogen.

	Structure of the nidogen binding LE module of the laminin gamma1 chain in solution.,Baumgartner R, Czisch M, Mayer U, Poschl E, Huber R, Timpl R, Holak TA J Mol Biol. 1996 Apr 5;257(3):658-68. PMID:8648631<ref>PMID:8648631</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1tle" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Laminin\|Laminin]]		*[[Laminin\|Laminin]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lk3 transgenic mice~~]]		[[Category: Mus musculus]]
	[[Category: Baumgartner, R]]		[[Category: Baumgartner R]]
	[[Category: Czisch, M]]		[[Category: Czisch M]]
	[[Category: Holak~~, T A~~]]		[[Category: Holak TA]]
	[[Category: Huber, R]]		[[Category: Huber R]]
	[[Category: Mayer, U]]		[[Category: Mayer U]]
	[[Category: Schl~~, E P~~]]		[[Category: Schl EP]]
	[[Category: Timpl, R]]		[[Category: Timpl R]]
	~~[[Category: Extracellular matrix protein]]~~
	~~[[Category: Glycoprotein]]~~
	~~[[Category: Le-module]]~~
	~~[[Category: Nidogen binding~~]]

OCA at 06:40, 2 March 2022

2022-03-02T06:40:22Z

← Older revision		Revision as of 06:40, 2 March 2022
Line 3:		Line 3:
	<StructureSection load='1tle' size='340' side='right'caption='[[1tle]], [[NMR_Ensembles_of_Models \| 14 NMR models]]' scene=''>		<StructureSection load='1tle' size='340' side='right'caption='[[1tle]], [[NMR_Ensembles_of_Models \| 14 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLE FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [~~http~~://pdbe.org/1tle PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1tle PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1tle ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [https://pdbe.org/1tle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [https://www.ebi.ac.uk/pdbsum/1tle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tle ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/LAMC1_MOUSE LAMC1_MOUSE]] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.		[[https://www.uniprot.org/uniprot/LAMC1_MOUSE LAMC1_MOUSE]] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:54, 24 December 2020

2020-12-24T11:54:58Z

← Older revision		Revision as of 11:54, 24 December 2020
Line 1:		Line 1:

	==LE (LAMININ-TYPE EGF-LIKE) MODULE GIII4 IN SOLUTION AT PH 3.5 AND 290 K, NMR, 14 STRUCTURES==		==LE (LAMININ-TYPE EGF-LIKE) MODULE GIII4 IN SOLUTION AT PH 3.5 AND 290 K, NMR, 14 STRUCTURES==
	<StructureSection load='1tle' size='340' side='right' caption='[[1tle]], [[NMR_Ensembles_of_Models \| 14 NMR models]]' scene=''>		<StructureSection load='1tle' size='340' side='right'caption='[[1tle]], [[NMR_Ensembles_of_Models \| 14 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1TLE FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [http://pdbe.org/1tle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [http://www.ebi.ac.uk/pdbsum/1tle PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tle ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [http://pdbe.org/1tle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [http://www.ebi.ac.uk/pdbsum/1tle PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tle ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1tle" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1tle" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Laminin\|Laminin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Baumgartner, R]]		[[Category: Baumgartner, R]]

OCA at 08:51, 21 March 2018

2018-03-21T08:51:12Z

← Older revision		Revision as of 08:51, 21 March 2018
Line 1:		Line 1:

	==LE (LAMININ-TYPE EGF-LIKE) MODULE GIII4 IN SOLUTION AT PH 3.5 AND 290 K, NMR, 14 STRUCTURES==		==LE (LAMININ-TYPE EGF-LIKE) MODULE GIII4 IN SOLUTION AT PH 3.5 AND 290 K, NMR, 14 STRUCTURES==
	<StructureSection load='1tle' size='340' side='right' caption='[[1tle]], [[NMR_Ensembles_of_Models \| 14 NMR models]]' scene=''>		<StructureSection load='1tle' size='340' side='right' caption='[[1tle]], [[NMR_Ensembles_of_Models \| 14 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TLE FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [http://pdbe.org/1tle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [http://www.ebi.ac.uk/pdbsum/1tle PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [http://pdbe.org/1tle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [http://www.ebi.ac.uk/pdbsum/1tle PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tle ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tle_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tle_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 04:51, 9 February 2016

2016-02-09T04:51:19Z

← Older revision		Revision as of 04:51, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tle ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:47, 10 September 2015

2015-09-10T18:47:39Z

← Older revision		Revision as of 18:47, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1tle' size='340' side='right' caption='[[1tle]], [[NMR_Ensembles_of_Models \| 14 NMR models]]' scene=''>		<StructureSection load='1tle' size='340' side='right' caption='[[1tle]], [[NMR_Ensembles_of_Models \| 14 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TLE FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [http://www.ebi.ac.uk/pdbsum/1tle PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [http://pdbe.org/1tle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [http://www.ebi.ac.uk/pdbsum/1tle PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1tle" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Mus musculus~~]]		[[Category: Lk3 transgenic mice]]
	[[Category: Baumgartner, R]]		[[Category: Baumgartner, R]]
	[[Category: Czisch, M]]		[[Category: Czisch, M]]

OCA at 04:33, 25 December 2014

2014-12-25T04:33:04Z

← Older revision		Revision as of 04:33, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TLE FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [http://www.ebi.ac.uk/pdbsum/1tle PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [http://www.ebi.ac.uk/pdbsum/1tle PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/LAMC1_MOUSE LAMC1_MOUSE]] Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Baumgartner, R.]]		[[Category: Baumgartner, R]]
	[[Category: Czisch, M.]]		[[Category: Czisch, M]]
	[[Category: Holak, T A.]]		[[Category: Holak, T A]]
	[[Category: Huber, R.]]		[[Category: Huber, R]]
	[[Category: Mayer, U.]]		[[Category: Mayer, U]]
	[[Category: Schl, E P.]]		[[Category: Schl, E P]]
	[[Category: Timpl, R.]]		[[Category: Timpl, R]]
	[[Category: Extracellular matrix protein]]		[[Category: Extracellular matrix protein]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
	[[Category: Le-module]]		[[Category: Le-module]]
	[[Category: Nidogen binding]]		[[Category: Nidogen binding]]

OCA at 20:05, 29 September 2014

2014-09-29T20:05:50Z

← Older revision		Revision as of 20:05, 29 September 2014
Line 1:		Line 1:
	[[Image:~~1tle~~.~~png~~\|~~left~~\|~~200px~~]]		==LE (LAMININ-TYPE EGF-LIKE) MODULE GIII4 IN SOLUTION AT PH 3.5 AND 290 K, NMR, 14 STRUCTURES==
			<StructureSection load='1tle' size='340' side='right' caption='[[1tle]], [[NMR_Ensembles_of_Models \| 14 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1tle]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TLE FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LAMC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tle OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tle RCSB], [http://www.ebi.ac.uk/pdbsum/1tle PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tle_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The structure of the single LE module between residues 791 and 848 of the laminin gamma1 chain, which contains the high affinity binding site for nidogen, has been probed using NMR methods. The module folds into an autonomous domain which has a stable and unique three-dimensional (3D) structure in solution. The 3D structure was determined on the basis of 362 interproton distance constraints derived from nuclear Overhauser enhancement measurements and 39 phi angles, supplemented by 5 psi and 22 chi1 angles. The main features of the NMR structures are two-stranded antiparallel beta-sheets which are separated by loops and cross-connected by four disulfide bridges. The N-terminal segment which contains the first three disulfide bridges is similar to epidermal growth factor. The C-terminal segment has an S-like backbone profile with a crossover at the last disulfide bridge and comprises two three-residue long beta-strands that form an antiparallel beta-sheet. The LE module possesses an exposed nidogen binding loop that projects away from the main body of the protein. The side-chains of three amino acids which are crucial for binding (Asp, Asn, Val) are all exposed at the domain surface. An inactivating Asn-Ser mutation in this region showed the same 3D structure indicating that these three residues, and possibly an additional Tyr in an adjacent loop, provide direct contacts in the interaction with nidogen.

	~~{{STRUCTURE_1tle\| PDB=1tle \| SCENE= }}~~		Structure of the nidogen binding LE module of the laminin gamma1 chain in solution.,Baumgartner R, Czisch M, Mayer U, Poschl E, Huber R, Timpl R, Holak TA J Mol Biol. 1996 Apr 5;257(3):658-68. PMID:8648631<ref>PMID:8648631</ref>

	~~===LE (LAMININ-TYPE EGF-LIKE) MODULE GIII4 IN SOLUTION AT PH 3.5 AND 290 K~~, ~~NMR, 14 STRUCTURES===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_8648631}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[1tle]] is~~ a ~~1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=1TLE OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:008648631<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Baumgartner, R.]]		[[Category: Baumgartner, R.]]

OCA: Protected "1tle" [edit=sysop:move=sysop]

2013-01-05T13:37:46Z

Protected "1tle" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:37, 5 January 2013
(No difference)