1two - Revision history

OCA at 04:55, 17 October 2024

2024-10-17T04:55:18Z

← Older revision		Revision as of 04:55, 17 October 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Antheraea_polyphemus Antheraea polyphemus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TWO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Antheraea_polyphemus Antheraea polyphemus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TWO FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [https://pdbe.org/1two PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [https://www.ebi.ac.uk/pdbsum/1two PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1two ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [https://pdbe.org/1two PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [https://www.ebi.ac.uk/pdbsum/1two PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1two ProSAT]</span></td></tr>
	</table>		</table>
Line 14:		Line 14:
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tw/1two_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tw/1two_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1two ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1two ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Olfaction in moths is one of the most impressive examples of chemical communication found in nature for its exquisite sensitivity and selectivity. Pheromone-binding proteins (PBPs), present in the antennae of male moth and other insect species, bind the hydrophobic pheromone molecules and transport them to the G protein-coupled olfactory receptor proteins. The targeted delivery of these non-polar ligands to membrane-bound receptors involves ligand release on or near the target cell membranes, the molecular details of which are still not well understood. The PBP from the giant silk moth Antheraea polyphemus (ApolPBP) binds acetate pheromone only at pH above 6.0, and its structure at pH 6.3 has been determined previously. Here we report the solution NMR structure of ApolPBP at the acidic pH 5.2. Comparison of the present structure to that at neutral pH reveals the details of the pH-induced conformational changes and provides mechanistic clues for ligand release at acidic pH. The ApolPBP pH-induced structural change is quite different from that observed for alcohol binding Bombyx mori PBP (BmorPBP), where the C-terminal segment folds into a helix and occupies the ligand binding cavity. We observe a reorientation of helices alpha1, alpha3, and alpha4 at acidic pH caused by protonation of His69, His70 and His95 in the interior. This provides the driving force behind the opening of the ligand binding cavity and the release of the pheromone molecule from its carrier protein near the membrane.

			Structural consequences of the pH-induced conformational switch in A.polyphemus pheromone-binding protein: mechanisms of ligand release.,Zubkov S, Gronenborn AM, Byeon IJ, Mohanty S J Mol Biol. 2005 Dec 16;354(5):1081-90. Epub 2005 Oct 27. PMID:16289114<ref>PMID:16289114</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 1two" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Pheromone binding protein\|Pheromone binding protein]]		*[[Pheromone binding protein\|Pheromone binding protein]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:45, 1 May 2024

2024-05-01T08:45:00Z

← Older revision		Revision as of 08:45, 1 May 2024
Line 1:		Line 1:

	==NMR structure of the pheromone binding protein from Antheraea polyphemus at acidic pH==		==NMR structure of the pheromone binding protein from Antheraea polyphemus at acidic pH==
	<StructureSection load='1two' size='340' side='right'caption='[[1two~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='1two' size='340' side='right'caption='[[1two]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Antpo Antpo~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TWO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Antheraea_polyphemus Antheraea polyphemus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TWO FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1qwv\|1qwv]], [[1gm0\|1gm0]]</div~~></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [https://pdbe.org/1two PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [https://www.ebi.ac.uk/pdbsum/1two PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1two ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [https://pdbe.org/1two PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [https://www.ebi.ac.uk/pdbsum/1two PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1two ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/PBP_ANTPO PBP_ANTPO]] This major soluble protein in olfactory sensilla of male moths might serve to solubilize the extremely hydrophobic pheromone molecules and to transport pheromone through the aqueous lymph to receptors located on olfactory cilia.		[https://www.uniprot.org/uniprot/PBP_ANTPO PBP_ANTPO] This major soluble protein in olfactory sensilla of male moths might serve to solubilize the extremely hydrophobic pheromone molecules and to transport pheromone through the aqueous lymph to receptors located on olfactory cilia.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1two ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1two ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Olfaction in moths is one of the most impressive examples of chemical communication found in nature for its exquisite sensitivity and selectivity. Pheromone-binding proteins (PBPs), present in the antennae of male moth and other insect species, bind the hydrophobic pheromone molecules and transport them to the G protein-coupled olfactory receptor proteins. The targeted delivery of these non-polar ligands to membrane-bound receptors involves ligand release on or near the target cell membranes, the molecular details of which are still not well understood. The PBP from the giant silk moth Antheraea polyphemus (ApolPBP) binds acetate pheromone only at pH above 6.0, and its structure at pH 6.3 has been determined previously. Here we report the solution NMR structure of ApolPBP at the acidic pH 5.2. Comparison of the present structure to that at neutral pH reveals the details of the pH-induced conformational changes and provides mechanistic clues for ligand release at acidic pH. The ApolPBP pH-induced structural change is quite different from that observed for alcohol binding Bombyx mori PBP (BmorPBP), where the C-terminal segment folds into a helix and occupies the ligand binding cavity. We observe a reorientation of helices alpha1, alpha3, and alpha4 at acidic pH caused by protonation of His69, His70 and His95 in the interior. This provides the driving force behind the opening of the ligand binding cavity and the release of the pheromone molecule from its carrier protein near the membrane.

	Structural consequences of the pH-induced conformational switch in A.polyphemus pheromone-binding protein: mechanisms of ligand release.,Zubkov S, Gronenborn AM, Byeon IJ, Mohanty S J Mol Biol. 2005 Dec 16;354(5):1081-90. Epub 2005 Oct 27. PMID:16289114<ref>PMID:16289114</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1two" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Pheromone binding protein\|Pheromone binding protein]]		*[[Pheromone binding protein\|Pheromone binding protein]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Antpo~~]]		[[Category: Antheraea polyphemus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mohanty, S]]		[[Category: Mohanty S]]
	[[Category: Zubkov, S]]		[[Category: Zubkov S]]
	~~[[Category: Apolpbp]]~~
	~~[[Category: Conformational switch]]~~
	~~[[Category: Conformational transition]]~~
	~~[[Category: Pbp]]~~
	~~[[Category: Pheromone binding protein~~]]

OCA at 09:33, 29 September 2021

2021-09-29T09:33:35Z

← Older revision		Revision as of 09:33, 29 September 2021
Line 3:		Line 3:
	<StructureSection load='1two' size='340' side='right'caption='[[1two]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1two' size='340' side='right'caption='[[1two]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Antpo Antpo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TWO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Antpo Antpo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TWO FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qwv\|1qwv]], [[1gm0\|1gm0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qwv\|1qwv]], [[1gm0\|1gm0]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [~~http~~://pdbe.org/1two PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1two PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1two ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [https://pdbe.org/1two PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [https://www.ebi.ac.uk/pdbsum/1two PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1two ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/PBP_ANTPO PBP_ANTPO]] This major soluble protein in olfactory sensilla of male moths might serve to solubilize the extremely hydrophobic pheromone molecules and to transport pheromone through the aqueous lymph to receptors located on olfactory cilia.		[[https://www.uniprot.org/uniprot/PBP_ANTPO PBP_ANTPO]] This major soluble protein in olfactory sensilla of male moths might serve to solubilize the extremely hydrophobic pheromone molecules and to transport pheromone through the aqueous lymph to receptors located on olfactory cilia.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 14:04, 25 December 2019

2019-12-25T14:04:40Z

← Older revision		Revision as of 14:04, 25 December 2019
Line 1:		Line 1:

	==NMR structure of the pheromone binding protein from Antheraea polyphemus at acidic pH==		==NMR structure of the pheromone binding protein from Antheraea polyphemus at acidic pH==
	<StructureSection load='1two' size='340' side='right' caption='[[1two]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1two' size='340' side='right'caption='[[1two]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antpo Antpo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antpo Antpo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWO FirstGlance]. <br>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Antpo]]		[[Category: Antpo]]
			[[Category: Large Structures]]
	[[Category: Mohanty, S]]		[[Category: Mohanty, S]]
	[[Category: Zubkov, S]]		[[Category: Zubkov, S]]

OCA at 08:54, 21 March 2018

2018-03-21T08:54:30Z

← Older revision		Revision as of 08:54, 21 March 2018
Line 1:		Line 1:

	==NMR structure of the pheromone binding protein from Antheraea polyphemus at acidic pH==		==NMR structure of the pheromone binding protein from Antheraea polyphemus at acidic pH==
	<StructureSection load='1two' size='340' side='right' caption='[[1two]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1two' size='340' side='right' caption='[[1two]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antpo Antpo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antpo Antpo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWO FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qwv\|1qwv]], [[1gm0\|1gm0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qwv\|1qwv]], [[1gm0\|1gm0]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [http://pdbe.org/1two PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [http://www.ebi.ac.uk/pdbsum/1two PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [http://pdbe.org/1two PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [http://www.ebi.ac.uk/pdbsum/1two PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1two ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tw/1two_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tw/1two_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:06, 8 February 2016

2016-02-08T03:06:03Z

← Older revision		Revision as of 03:06, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1two ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:16, 10 September 2015

2015-09-10T07:16:35Z

← Older revision		Revision as of 07:16, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1two' size='340' side='right' caption='[[1two]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1two' size='340' side='right' caption='[[1two]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Antheraea_polyphemus Antheraea polyphemus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antpo Antpo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWO FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qwv\|1qwv]], [[1gm0\|1gm0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qwv\|1qwv]], [[1gm0\|1gm0]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [http://www.ebi.ac.uk/pdbsum/1two PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [http://pdbe.org/1two PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [http://www.ebi.ac.uk/pdbsum/1two PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1two" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Pheromone binding protein\|Pheromone binding protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Antheraea polyphemus~~]]		[[Category: Antpo]]
	[[Category: Mohanty, S]]		[[Category: Mohanty, S]]
	[[Category: Zubkov, S]]		[[Category: Zubkov, S]]

OCA at 20:07, 24 December 2014

2014-12-24T20:07:45Z

← Older revision		Revision as of 20:07, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antheraea_polyphemus Antheraea polyphemus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWO FirstGlance]. <br>		<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antheraea_polyphemus Antheraea polyphemus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWO FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qwv\|1qwv]], [[1gm0\|1gm0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qwv\|1qwv]], [[1gm0\|1gm0]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [http://www.ebi.ac.uk/pdbsum/1two PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [http://www.ebi.ac.uk/pdbsum/1two PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/PBP_ANTPO PBP_ANTPO]] This major soluble protein in olfactory sensilla of male moths might serve to solubilize the extremely hydrophobic pheromone molecules and to transport pheromone through the aqueous lymph to receptors located on olfactory cilia.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Antheraea polyphemus]]		[[Category: Antheraea polyphemus]]
	[[Category: Mohanty, S.]]		[[Category: Mohanty, S]]
	[[Category: Zubkov, S.]]		[[Category: Zubkov, S]]
	[[Category: Apolpbp]]		[[Category: Apolpbp]]
	[[Category: Conformational switch]]		[[Category: Conformational switch]]

OCA at 20:38, 29 September 2014

2014-09-29T20:38:22Z

← Older revision		Revision as of 20:38, 29 September 2014
Line 1:		Line 1:
	[[Image:~~1two~~.~~png~~\|~~left~~\|~~200px~~]]		==NMR structure of the pheromone binding protein from Antheraea polyphemus at acidic pH==
			<StructureSection load='1two' size='340' side='right' caption='[[1two]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1two]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Antheraea_polyphemus Antheraea polyphemus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWO FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qwv\|1qwv]], [[1gm0\|1gm0]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1two FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1two OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1two RCSB], [http://www.ebi.ac.uk/pdbsum/1two PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tw/1two_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Olfaction in moths is one of the most impressive examples of chemical communication found in nature for its exquisite sensitivity and selectivity. Pheromone-binding proteins (PBPs), present in the antennae of male moth and other insect species, bind the hydrophobic pheromone molecules and transport them to the G protein-coupled olfactory receptor proteins. The targeted delivery of these non-polar ligands to membrane-bound receptors involves ligand release on or near the target cell membranes, the molecular details of which are still not well understood. The PBP from the giant silk moth Antheraea polyphemus (ApolPBP) binds acetate pheromone only at pH above 6.0, and its structure at pH 6.3 has been determined previously. Here we report the solution NMR structure of ApolPBP at the acidic pH 5.2. Comparison of the present structure to that at neutral pH reveals the details of the pH-induced conformational changes and provides mechanistic clues for ligand release at acidic pH. The ApolPBP pH-induced structural change is quite different from that observed for alcohol binding Bombyx mori PBP (BmorPBP), where the C-terminal segment folds into a helix and occupies the ligand binding cavity. We observe a reorientation of helices alpha1, alpha3, and alpha4 at acidic pH caused by protonation of His69, His70 and His95 in the interior. This provides the driving force behind the opening of the ligand binding cavity and the release of the pheromone molecule from its carrier protein near the membrane.

	~~{{STRUCTURE_1two\| PDB=1two \| SCENE= }}~~		Structural consequences of the pH-induced conformational switch in A.polyphemus pheromone-binding protein: mechanisms of ligand release.,Zubkov S, Gronenborn AM, Byeon IJ, Mohanty S J Mol Biol. 2005 Dec 16;354(5):1081-90. Epub 2005 Oct 27. PMID:16289114<ref>PMID:16289114</ref>

	~~===NMR structure~~ of the ~~pheromone binding protein from Antheraea polyphemus at acidic pH===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16289114}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[1two]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Antheraea_polyphemus Antheraea polyphemus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1TWO OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016289114<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Antheraea polyphemus]]		[[Category: Antheraea polyphemus]]
	[[Category: Mohanty, S.]]		[[Category: Mohanty, S.]]

OCA: Protected "1two" [edit=sysop:move=sysop]

2013-01-05T11:25:31Z

Protected "1two" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:25, 5 January 2013
(No difference)