1x77 - Revision history

OCA at 06:42, 23 August 2023

2023-08-23T06:42:08Z

← Older revision		Revision as of 06:42, 23 August 2023
Line 1:		Line 1:

	==Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN==		==Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN==
	<StructureSection load='1x77' size='340' side='right'caption='[[1x77]]' scene=''>		<StructureSection load='1x77' size='340' side='right'caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X77 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [https://pdbe.org/1x77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [https://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x77 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [https://pdbe.org/1x77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [https://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x77 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/FMNRE_PSEAE FMNRE_PSEAE] Has NAD(P)H-dependent FMN reductase activity.<ref>PMID:16552139</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x77 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x77 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.

			Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal.,Agarwal R, Bonanno JB, Burley SK, Swaminathan S Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:16552139<ref>PMID:16552139</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 1x77" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Pseudomonas aeruginosa PAO1]]
	[[Category: Agarwal R]]		[[Category: Agarwal R]]
	[[Category: Burley SK]]		[[Category: Burley SK]]
	[[Category: Swaminathan S]]		[[Category: Swaminathan S]]

OCA at 11:30, 30 March 2022

2022-03-30T11:30:27Z

← Older revision		Revision as of 11:30, 30 March 2022
Line 1:		Line 1:

	==Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN==		==Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN==
	<StructureSection load='1x77' size='340' side='right'caption='[[1x77]]~~, [[Resolution\|resolution]] 2.70Å~~' scene=''>		<StructureSection load='1x77' size='340' side='right'caption='[[1x77]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1X77 FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X77 FirstGlance]. <br>
	</td></tr>~~<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [https://pdbe.org/1x77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [https://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x77 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>~~
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [~~http~~://pdbe.org/1x77 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1x77 ProSAT], [~~http~~://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 18:		Line 16:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x77 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x77 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.

	Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal.,Agarwal R, Bonanno JB, Burley SK, Swaminathan S Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:16552139<ref>PMID:16552139</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 1x77" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Pseae]]~~		[[Category: Agarwal R]]
	[[Category: Agarwal, R]]		[[Category: Burley SK]]
	[[Category: Burley~~, S K]]~~		[[Category: Swaminathan S]]
	~~[[Category: Structural genomic~~]]
	[[Category: Swaminathan, S]]
	~~[[Category: Fmn bound]]~~
	~~[[Category: Fmn reductase]]~~
	~~[[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]]~~
	~~[[Category: Unknown function~~]]

OCA at 07:35, 25 June 2020

2020-06-25T07:35:11Z

← Older revision		Revision as of 07:35, 25 June 2020
Line 1:		Line 1:

	==Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN==		==Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN==
	<StructureSection load='1x77' size='340' side='right' caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1x77' size='340' side='right'caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1X77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1X77 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://pdbe.org/1x77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1x77 ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://pdbe.org/1x77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1x77 ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Agarwal, R]]		[[Category: Agarwal, R]]

OCA at 21:16, 19 September 2018

2018-09-19T21:16:22Z

← Older revision		Revision as of 21:16, 19 September 2018
Line 3:		Line 3:
	<StructureSection load='1x77' size='340' side='right' caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1x77' size='340' side='right' caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X77 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/1x77_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/1x77_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Pseae]]
	[[Category: Agarwal, R]]		[[Category: Agarwal, R]]
	[[Category: Burley, S K]]		[[Category: Burley, S K]]

OCA at 11:29, 15 December 2016

2016-12-15T11:29:37Z

← Older revision		Revision as of 11:29, 15 December 2016
Line 1:		Line 1:
	==Crystal ~~struture~~ of a NAD(P)H-dependent FMN reductase complexed with FMN==
			==Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN==
	<StructureSection load='1x77' size='340' side='right' caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1x77' size='340' side='right' caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X77 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://pdbe.org/1x77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://pdbe.org/1x77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1x77 ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x77 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Pseae]]~~
	[[Category: Agarwal, R]]		[[Category: Agarwal, R]]
	[[Category: Burley, S K]]		[[Category: Burley, S K]]

OCA at 15:33, 11 September 2015

2015-09-11T15:33:36Z

← Older revision		Revision as of 15:33, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1x77' size='340' side='right' caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1x77' size='340' side='right' caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X77 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://pdbe.org/1x77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1x77" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Pseudomonas aeruginosa~~]]		[[Category: Pseae]]
	[[Category: Agarwal, R]]		[[Category: Agarwal, R]]
	[[Category: Burley, S K]]		[[Category: Burley, S K]]

OCA at 12:57, 6 November 2014

2014-11-06T12:57:15Z

← Older revision		Revision as of 12:57, 6 November 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X77 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X77 FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Agarwal, R.]]		[[Category: Agarwal, R]]
	[[Category: Burley, S K.]]		[[Category: Burley, S K]]
	[[Category: ~~NYSGXRC, New York SGX Research Center for~~ Structural ~~Genomics.~~]]		[[Category: Structural genomic]]
	[[Category: Swaminathan, S.]]		[[Category: Swaminathan, S]]
	[[Category: Fmn bound]]		[[Category: Fmn bound]]
	[[Category: Fmn reductase]]		[[Category: Fmn reductase]]
	[[Category: New ~~york sgx research center~~ for ~~structural genomic]]~~		[[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]]
	~~[[Category: Nysgxrc]]~~
	~~[[Category:~~ Structural ~~genomic~~]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 21:36, 29 September 2014

2014-09-29T21:36:58Z

← Older revision		Revision as of 21:36, 29 September 2014
Line 1:		Line 1:
	[[Image:~~1x77~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal struture of a NAD(P)H-dependent FMN reductase complexed with FMN==
			<StructureSection load='1x77' size='340' side='right' caption='[[1x77]], [[Resolution\|resolution]] 2.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X77 FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rtt\|1rtt]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/1x77_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.

	~~{{STRUCTURE_1x77\| PDB=1x77 \| SCENE= }}~~		Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal.,Agarwal R, Bonanno JB, Burley SK, Swaminathan S Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:16552139<ref>PMID:16552139</ref>

	~~===Crystal struture~~ of ~~a NAD(P)H-dependent FMN reductase complexed with FMN===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16552139}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[1x77]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016552139<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Agarwal, R.]]		[[Category: Agarwal, R.]]

OCA: Protected "1x77" [edit=sysop:move=sysop]

2013-01-05T12:57:42Z

Protected "1x77" [edit=sysop:move=sysop]

← Older revision	Revision as of 12:57, 5 January 2013
(No difference)

OCA at 12:57, 5 January 2013

2013-01-05T12:57:39Z

← Older revision		Revision as of 12:57, 5 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:1x77.png\|left\|200px]]		[[Image:1x77.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_1x77", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_1x77\| PDB=1x77 \| SCENE= }}		{{STRUCTURE_1x77\| PDB=1x77 \| SCENE= }}

	===Crystal struture of a NAD(P)H-dependent FMN reductase complexed with FMN===		===Crystal struture of a NAD(P)H-dependent FMN reductase complexed with FMN===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16552139}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16552139 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16552139}}		{{ABSTRACT_PUBMED_16552139}}

	==About this Structure==		==About this Structure==
	~~1X77~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa_pao1~~ Pseudomonas aeruginosa ~~pao1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA].		[[1x77]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16552139~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016552139</ref><references group="xtra"/>
	[[Category: Pseudomonas aeruginosa ~~pao1~~]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Agarwal, R.]]		[[Category: Agarwal, R.]]
	[[Category: Burley, S K.]]		[[Category: Burley, S K.]]
	[[Category: NYSGXRC, New York Structural ~~GenomiX Research Consortium~~.]]		[[Category: NYSGXRC, New York SGX Research Center for Structural Genomics.]]
	[[Category: Swaminathan, S.]]		[[Category: Swaminathan, S.]]
	[[Category: Fmn bound]]		[[Category: Fmn bound]]
	[[Category: Fmn reductase]]		[[Category: Fmn reductase]]
	[[Category: New york structural ~~genomix research consortium~~]]		[[Category: New york sgx research center for structural genomic]]
	[[Category: Nysgxrc]]		[[Category: Nysgxrc]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
			[[Category: Unknown function]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 02:24:51 2009''~~