1ync - Revision history

OCA at 09:30, 6 December 2023

2023-12-06T09:30:55Z

← Older revision		Revision as of 09:30, 6 December 2023
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	==NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor==		==NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor==
	<StructureSection load='1ync' size='340' side='right'caption='[[1ync~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>		<StructureSection load='1ync' size='340' side='right'caption='[[1ync]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNC FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</div~~></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [https://pdbe.org/1ync PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [https://www.ebi.ac.uk/pdbsum/1ync PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ync ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [https://pdbe.org/1ync PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [https://www.ebi.ac.uk/pdbsum/1ync PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ync ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Allain~~, F H~~]]		[[Category: Allain FH]]
	[[Category: Chester, A]]		[[Category: Chester A]]
	[[Category: Maris, C]]		[[Category: Maris C]]
	[[Category: Masse, J]]		[[Category: Masse J]]
	[[Category: Navaratnam, N]]		[[Category: Navaratnam N]]
	~~[[Category: Acf]]~~
	~~[[Category: Apob mrna]]~~
	~~[[Category: Apobec1]]~~
	~~[[Category: Nmr structure]]~~
	~~[[Category: Rna]]~~
	~~[[Category: Rna editing~~]]

OCA at 16:30, 3 November 2021

2021-11-03T16:30:28Z

← Older revision		Revision as of 16:30, 3 November 2021
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	<StructureSection load='1ync' size='340' side='right'caption='[[1ync]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1ync' size='340' side='right'caption='[[1ync]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1YNC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNC FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [~~http~~://pdbe.org/1ync PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ync PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ync ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [https://pdbe.org/1ync PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [https://www.ebi.ac.uk/pdbsum/1ync PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ync ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:03, 29 January 2020

2020-01-29T17:03:34Z

← Older revision		Revision as of 17:03, 29 January 2020
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	==NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor==		==NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor==
	<StructureSection load='1ync' size='340' side='right' caption='[[1ync]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1ync' size='340' side='right'caption='[[1ync]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNC FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Allain, F H]]		[[Category: Allain, F H]]
	[[Category: Chester, A]]		[[Category: Chester, A]]

OCA at 06:47, 25 April 2018

2018-04-25T06:47:38Z

← Older revision		Revision as of 06:47, 25 April 2018
Line 1:		Line 1:

	==NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor==		==NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor==
	<StructureSection load='1ync' size='340' side='right' caption='[[1ync]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1ync' size='340' side='right' caption='[[1ync]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
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	<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNC FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [http://pdbe.org/1ync PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [http://www.ebi.ac.uk/pdbsum/1ync PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [http://pdbe.org/1ync PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [http://www.ebi.ac.uk/pdbsum/1ync PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ync ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:11, 10 September 2015

2015-09-10T17:11:45Z

← Older revision		Revision as of 17:11, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNC FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [http://www.ebi.ac.uk/pdbsum/1ync PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [http://pdbe.org/1ync PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [http://www.ebi.ac.uk/pdbsum/1ync PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 1ync" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:25, 22 December 2014

2014-12-22T06:25:07Z

← Older revision		Revision as of 06:25, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNC FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [http://www.ebi.ac.uk/pdbsum/1ync PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [http://www.ebi.ac.uk/pdbsum/1ync PDBsum]</span></td></tr>
	<table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Allain, F H.]]		[[Category: Allain, F H]]
	[[Category: Chester, A.]]		[[Category: Chester, A]]
	[[Category: Maris, C.]]		[[Category: Maris, C]]
	[[Category: Masse, J.]]		[[Category: Masse, J]]
	[[Category: Navaratnam, N.]]		[[Category: Navaratnam, N]]
	[[Category: Acf]]		[[Category: Acf]]
	[[Category: Apob mrna]]		[[Category: Apob mrna]]

OCA at 07:01, 9 June 2014

2014-06-09T07:01:06Z

← Older revision		Revision as of 07:01, 9 June 2014
Line 1:		Line 1:
	[[~~Image~~:~~1ync~~.~~png~~\|~~left~~\|~~200px~~]]		==NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor==
			<StructureSection load='1ync' size='340' side='right' caption='[[1ync]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YNC FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ylg\|1ylg]], [[1yne\|1yne]], [[1yng\|1yng]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ync FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ync OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ync RCSB], [http://www.ebi.ac.uk/pdbsum/1ync PDBsum]</span></td></tr>
			<table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			We have solved the NMR structure of the 31-nucleotide (nt) apoB mRNA stem-loop, a substrate of the cytidine deaminase APOBEC1. We found that the edited base located at the 5' end of the octa-loop is stacked between two adenosines in both the unedited (cytidine 6666) and the edited (uridine 6666) forms and that the rest of the loop is unstructured. The 11-nt "mooring" sequence essential for editing is partially flexible although it is mostly in the stem of the RNA. The octa-loop and the internal loop in the middle of the stem confer this flexibility. These findings shed light on why APOBEC1 alone cannot edit efficiently the cytidine 6666 under physiological conditions, the editing base being buried in the loop and not directly accessible. We also show that APOBEC1 does not specifically bind apoB mRNA and requires the auxiliary factor, APOBEC1 complementary factor (ACF), to edit specifically cytidine 6666. The binding of ACF to both the mooring sequence and APOBEC1 explains the specificity of the reaction. Our NMR study lead us to propose a mechanism in which ACF recognizes first the flexible nucleotides of the mooring sequence (the internal loop and the 3' end octa-loop) and subsequently melts the stem-loop, exposing the amino group of the cytidine 6666 to APOBEC1. Thus, the flexibility of the mooring sequence plays a central role in the RNA recognition by ACF.

	~~{{STRUCTURE_1ync\| PDB=1ync \| SCENE= }}~~		NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor.,Maris C, Masse J, Chester A, Navaratnam N, Allain FH RNA. 2005 Feb;11(2):173-86. PMID:15659357<ref>PMID:15659357</ref>

	~~===NMR structure~~ of the ~~apoB mRNA stem-loop and its interaction with the C to~~ U ~~editing APOBEC1 complementary factor===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_15659357}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1YNC OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:015659357<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Allain, F H.]]		[[Category: Allain, F H.]]
	[[Category: Chester, A.]]		[[Category: Chester, A.]]

OCA: Protected "1ync" [edit=sysop:move=sysop]

2012-08-14T18:28:24Z

Protected "1ync" [edit=sysop:move=sysop]

← Older revision	Revision as of 18:28, 14 August 2012
(No difference)

OCA at 18:28, 14 August 2012

2012-08-14T18:28:22Z

← Older revision		Revision as of 18:28, 14 August 2012
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	~~{{Seed}}~~
	[[Image:1ync.png\|left\|200px]]		[[Image:1ync.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_1ync", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_1ync\| PDB=1ync \| SCENE= }}		{{STRUCTURE_1ync\| PDB=1ync \| SCENE= }}

	===NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor===		===NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_15659357}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 15659357 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_15659357}}		{{ABSTRACT_PUBMED_15659357}}

	==About this Structure==		==About this Structure==
	~~1YNC is a~~ [[~~Single protein~~]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA].		[[1ync]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA].

	==Reference==		==Reference==
	~~NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor., Maris C, Masse J, Chester A, Navaratnam N, Allain FH, RNA. 2005 Feb;11(2):173-86.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15659357 15659357]~~		<ref group="xtra">PMID:015659357</ref><references group="xtra"/>
	~~[[Category: Single protein]]~~
	[[Category: Allain, F H.]]		[[Category: Allain, F H.]]
	[[Category: Chester, A.]]		[[Category: Chester, A.]]
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	[[Category: Apobec1]]		[[Category: Apobec1]]
	[[Category: Nmr structure]]		[[Category: Nmr structure]]
			[[Category: Rna]]
	[[Category: Rna editing]]		[[Category: Rna editing]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:36:53 2008''~~

OCA at 05:37, 28 July 2008

2008-07-28T05:37:02Z

← Older revision		Revision as of 05:37, 28 July 2008
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	[[Image:1ync.~~gif~~\|left\|200px]]		{{Seed}}
			[[Image:1ync.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1ync\| PDB=1ync \| SCENE= }}		{{STRUCTURE_1ync\| PDB=1ync \| SCENE= }}

	~~'''~~NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor~~'''~~		===NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor===


	~~==Overview==~~		<!--
	~~We have solved the NMR structure of the 31~~-~~nucleotide (nt) apoB mRNA stem~~-~~loop~~, ~~a substrate of~~ the ~~cytidine deaminase APOBEC1. We found that~~ the ~~edited base located at the 5' end of the octa-loop is stacked between two adenosines in both the unedited (cytidine 6666) and the edited~~ (~~uridine 6666) forms and that the rest of the loop is unstructured. The 11-nt "mooring" sequence essential for editing is partially flexible although~~ it ~~is mostly in the stem of the RNA. The octa-loop and the internal loop in the middle of the stem confer this flexibility~~. ~~These findings shed light on why APOBEC1 alone cannot edit efficiently the cytidine 6666 under physiological conditions, the editing base being buried in the loop and not directly accessible~~. ~~We also show that APOBEC1 does not specifically bind apoB mRNA and requires the auxiliary factor, APOBEC1 complementary factor (ACF~~), ~~to edit specifically cytidine 6666. The binding of ACF to both the mooring sequence and APOBEC1 explains the specificity of~~ the ~~reaction~~. ~~Our NMR study lead us to propose a mechanism in which ACF recognizes first the flexible nucleotides of the mooring sequence (the internal loop and the 3' end octa~~-~~loop) and subsequently melts the stem~~-~~loop, exposing the amino group of the cytidine 6666 to APOBEC1. Thus, the flexibility of the mooring sequence plays a central role in the RNA recognition by ACF.~~		The line below this paragraph, {{ABSTRACT_PUBMED_15659357}}, adds the Publication Abstract to the page
			(as it appears on PubMed at http://www.pubmed.gov), where 15659357 is the PubMed ID number.
			-->
			{{ABSTRACT_PUBMED_15659357}}

	==About this Structure==		==About this Structure==
	1YNC is a [[Single protein]] structure. Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA].		1YNC is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNC OCA].

	==Reference==		==Reference==
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	[[Category: Nmr structure]]		[[Category: Nmr structure]]
	[[Category: Rna editing]]		[[Category: Rna editing]]
	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 16~~:32:25 2008''
			''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:36:53 2008''