2a3f - Revision history

OCA at 00:45, 21 November 2024

2024-11-21T00:45:47Z

← Older revision		Revision as of 00:45, 21 November 2024
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	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3f_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3f_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/~~initialview01~~.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3f ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Nitrophorin 2 (NP2) (also known as prolixin-S) is a salivary protein that transports nitric oxide, binds histamine, and acts as an anticoagulant during blood feeding by the insect Rhodnius prolixus. The 2.0-A crystal structure of NP2 reveals an eight-stranded antiparallel beta-barrel containing a ferric heme coordinated through His(57), similar to the structures of NP1 and NP4. All four Rhodnius nitrophorins transport NO and sequester histamine through heme binding, but only NP2 acts as an anticoagulant. Here, we demonstrate that recombinant NP2, but not recombinant NP1 or NP4, is a potent anticoagulant; recombinant NP3 also displays minor activity. Comparison of the nitrophorin structures suggests that a surface region near the C terminus and the loops between beta strands B-C and E-F is responsible for the anticoagulant activity. NP2 also displays larger NO association rates and smaller dissociation rates than NP1 and NP4, which may result from a more open and more hydrophobic distal pocket, allowing more rapid solvent reorganization on ligand binding. The NP2 protein core differs from NP1 and NP4 in that buried Glu(53), which allows for larger NO release rates when deprotonated, hydrogen bonds to invariant Tyr(81). Surprisingly, this tyrosine lies on the protein surface in NP1 and NP4.

			The crystal structure of nitrophorin 2. A trifunctional antihemostatic protein from the saliva of Rhodnius prolixus.,Andersen JF, Montfort WR J Biol Chem. 2000 Sep 29;275(39):30496-503. PMID:010884386<ref>PMID:010884386</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 2a3f" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Nitrophorin\|Nitrophorin]]		*[[Nitrophorin\|Nitrophorin]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:18, 23 August 2023

2023-08-23T07:18:16Z

← Older revision		Revision as of 07:18, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='2a3f' size='340' side='right'caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2a3f' size='340' side='right'caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Rhopr Rhopr~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A3F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A3F FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [https://pdbe.org/2a3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [https://www.ebi.ac.uk/pdbsum/2a3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [https://pdbe.org/2a3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [https://www.ebi.ac.uk/pdbsum/2a3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/NP2_RHOPR NP2_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity). Specifically inhibits factor IXa-catalyzed activation of factor X in the presence of calcium and phospholipids irrespective of the presence or absence of factor VIIIa.		[https://www.uniprot.org/uniprot/NP2_RHOPR NP2_RHOPR] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity). Specifically inhibits factor IXa-catalyzed activation of factor X in the presence of calcium and phospholipids irrespective of the presence or absence of factor VIIIa.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3f ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Nitrophorin 2 (NP2) (also known as prolixin-S) is a salivary protein that transports nitric oxide, binds histamine, and acts as an anticoagulant during blood feeding by the insect Rhodnius prolixus. The 2.0-A crystal structure of NP2 reveals an eight-stranded antiparallel beta-barrel containing a ferric heme coordinated through His(57), similar to the structures of NP1 and NP4. All four Rhodnius nitrophorins transport NO and sequester histamine through heme binding, but only NP2 acts as an anticoagulant. Here, we demonstrate that recombinant NP2, but not recombinant NP1 or NP4, is a potent anticoagulant; recombinant NP3 also displays minor activity. Comparison of the nitrophorin structures suggests that a surface region near the C terminus and the loops between beta strands B-C and E-F is responsible for the anticoagulant activity. NP2 also displays larger NO association rates and smaller dissociation rates than NP1 and NP4, which may result from a more open and more hydrophobic distal pocket, allowing more rapid solvent reorganization on ligand binding. The NP2 protein core differs from NP1 and NP4 in that buried Glu(53), which allows for larger NO release rates when deprotonated, hydrogen bonds to invariant Tyr(81). Surprisingly, this tyrosine lies on the protein surface in NP1 and NP4.

	The crystal structure of nitrophorin 2. A trifunctional antihemostatic protein from the saliva of Rhodnius prolixus.,Andersen JF, Montfort WR J Biol Chem. 2000 Sep 29;275(39):30496-503. PMID:10884386<ref>PMID:10884386</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2a3f" style="background-color:#fffaf0;"></div>~~

	==See Also==		==See Also==
	*[[Nitrophorin\|Nitrophorin]]		*[[Nitrophorin\|Nitrophorin]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Rhopr~~]]		[[Category: Rhodnius prolixus]]
	[[Category: Berry~~, R E~~]]		[[Category: Berry RE]]
	[[Category: Montfort~~, W R~~]]		[[Category: Montfort WR]]
	[[Category: Walker~~, F A~~]]		[[Category: Walker FA]]
	[[Category: Weichsel, A]]		[[Category: Weichsel A]]
	~~[[Category: Beta barrel]]~~
	~~[[Category: Ferric heme]]~~
	~~[[Category: Lipocalin]]~~
	~~[[Category: Transport protein~~]]

OCA at 14:35, 17 November 2021

2021-11-17T14:35:42Z

← Older revision		Revision as of 14:35, 17 November 2021
Line 3:		Line 3:
	<StructureSection load='2a3f' size='340' side='right'caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2a3f' size='340' side='right'caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2A3F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A3F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [~~http~~://pdbe.org/2a3f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2a3f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [https://pdbe.org/2a3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [https://www.ebi.ac.uk/pdbsum/2a3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/NP2_RHOPR NP2_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity). Specifically inhibits factor IXa-catalyzed activation of factor X in the presence of calcium and phospholipids irrespective of the presence or absence of factor VIIIa.		[[https://www.uniprot.org/uniprot/NP2_RHOPR NP2_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity). Specifically inhibits factor IXa-catalyzed activation of factor X in the presence of calcium and phospholipids irrespective of the presence or absence of factor VIIIa.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 05:17, 13 February 2020

2020-02-13T05:17:36Z

← Older revision		Revision as of 05:17, 13 February 2020
Line 1:		Line 1:

	==Crystal structure of nitrophorin 2 aqua complex==		==Crystal structure of nitrophorin 2 aqua complex==
	<StructureSection load='2a3f' size='340' side='right' caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2a3f' size='340' side='right'caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A3F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A3F FirstGlance]. <br>
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Rhopr]]		[[Category: Rhopr]]
	[[Category: Berry, R E]]		[[Category: Berry, R E]]

OCA at 07:08, 9 May 2018

2018-05-09T07:08:42Z

← Older revision		Revision as of 07:08, 9 May 2018
Line 1:		Line 1:

	==Crystal structure of nitrophorin 2 aqua complex==		==Crystal structure of nitrophorin 2 aqua complex==
	<StructureSection load='2a3f' size='340' side='right' caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2a3f' size='340' side='right' caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [http://pdbe.org/2a3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [http://www.ebi.ac.uk/pdbsum/2a3f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [http://pdbe.org/2a3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [http://www.ebi.ac.uk/pdbsum/2a3f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3f_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3f_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 2a3f" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2a3f" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Nitrophorin\|Nitrophorin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:04, 11 September 2015

2015-09-11T13:04:55Z

← Older revision		Revision as of 13:04, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2a3f' size='340' side='right' caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='2a3f' size='340' side='right' caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rhodnius_prolixus Rhodnius prolixus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A3F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A3F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [http://www.ebi.ac.uk/pdbsum/2a3f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [http://pdbe.org/2a3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [http://www.ebi.ac.uk/pdbsum/2a3f PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2a3f" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Rhodnius prolixus~~]]		[[Category: Rhopr]]
	[[Category: Berry, R E]]		[[Category: Berry, R E]]
	[[Category: Montfort, W R]]		[[Category: Montfort, W R]]

OCA at 16:50, 25 December 2014

2014-12-25T16:50:12Z

← Older revision		Revision as of 16:50, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A3F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A3F FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [http://www.ebi.ac.uk/pdbsum/2a3f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [http://www.ebi.ac.uk/pdbsum/2a3f PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/NP2_RHOPR NP2_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity). Specifically inhibits factor IXa-catalyzed activation of factor X in the presence of calcium and phospholipids irrespective of the presence or absence of factor VIIIa.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Rhodnius prolixus]]		[[Category: Rhodnius prolixus]]
	[[Category: Berry, R E.]]		[[Category: Berry, R E]]
	[[Category: Montfort, W R.]]		[[Category: Montfort, W R]]
	[[Category: Walker, F A.]]		[[Category: Walker, F A]]
	[[Category: Weichsel, A.]]		[[Category: Weichsel, A]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Ferric heme]]		[[Category: Ferric heme]]
	[[Category: Lipocalin]]		[[Category: Lipocalin]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 02:46, 30 September 2014

2014-09-30T02:46:09Z

← Older revision		Revision as of 02:46, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2a3f~~.~~png~~\|~~left~~\|~~200px~~]]		==Crystal structure of nitrophorin 2 aqua complex==
			<StructureSection load='2a3f' size='340' side='right' caption='[[2a3f]], [[Resolution\|resolution]] 1.40Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2a3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A3F FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1euo\|1euo]], [[1t68\|1t68]], [[1pee\|1pee]], [[1pm1\|1pm1]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a3f RCSB], [http://www.ebi.ac.uk/pdbsum/2a3f PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3f_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Nitrophorin 2 (NP2) (also known as prolixin-S) is a salivary protein that transports nitric oxide, binds histamine, and acts as an anticoagulant during blood feeding by the insect Rhodnius prolixus. The 2.0-A crystal structure of NP2 reveals an eight-stranded antiparallel beta-barrel containing a ferric heme coordinated through His(57), similar to the structures of NP1 and NP4. All four Rhodnius nitrophorins transport NO and sequester histamine through heme binding, but only NP2 acts as an anticoagulant. Here, we demonstrate that recombinant NP2, but not recombinant NP1 or NP4, is a potent anticoagulant; recombinant NP3 also displays minor activity. Comparison of the nitrophorin structures suggests that a surface region near the C terminus and the loops between beta strands B-C and E-F is responsible for the anticoagulant activity. NP2 also displays larger NO association rates and smaller dissociation rates than NP1 and NP4, which may result from a more open and more hydrophobic distal pocket, allowing more rapid solvent reorganization on ligand binding. The NP2 protein core differs from NP1 and NP4 in that buried Glu(53), which allows for larger NO release rates when deprotonated, hydrogen bonds to invariant Tyr(81). Surprisingly, this tyrosine lies on the protein surface in NP1 and NP4.

	~~{{STRUCTURE_2a3f\| PDB=2a3f \| SCENE= }}~~		The crystal structure of nitrophorin 2. A trifunctional antihemostatic protein from the saliva of Rhodnius prolixus.,Andersen JF, Montfort WR J Biol Chem. 2000 Sep 29;275(39):30496-503. PMID:10884386<ref>PMID:10884386</ref>

	~~===Crystal structure~~ of ~~nitrophorin 2 aqua complex===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_10884386}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2a3f]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rhodnius_prolixus Rhodnius prolixus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:010884386<~~/~~ref><ref group="xtra">PMID:019140692</ref~~><~~references group="xtra"~~/>
	[[Category: Rhodnius prolixus]]		[[Category: Rhodnius prolixus]]
	[[Category: Berry, R E.]]		[[Category: Berry, R E.]]

OCA: Protected "2a3f" [edit=sysop:move=sysop]

2013-01-06T11:57:55Z

Protected "2a3f" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:57, 6 January 2013
(No difference)

OCA at 11:57, 6 January 2013

2013-01-06T11:57:50Z

← Older revision		Revision as of 11:57, 6 January 2013
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	~~{{Seed}}~~
	[[Image:2a3f.png\|left\|200px]]		[[Image:2a3f.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2a3f", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2a3f\| PDB=2a3f \| SCENE= }}		{{STRUCTURE_2a3f\| PDB=2a3f \| SCENE= }}

	===Crystal structure of nitrophorin 2 aqua complex===		===Crystal structure of nitrophorin 2 aqua complex===

			{{ABSTRACT_PUBMED_10884386}}

	==About this Structure==		==About this Structure==
	~~2A3F~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA].		[[2a3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3F OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~10884386~~</ref><references group="xtra"/>		<ref group="xtra">PMID:010884386</ref><ref group="xtra">PMID:019140692</ref><references group="xtra"/>
	[[Category: Rhodnius prolixus]]		[[Category: Rhodnius prolixus]]
	[[Category: Berry, R E.]]		[[Category: Berry, R E.]]
Line 26:		Line 20:
	[[Category: Ferric heme]]		[[Category: Ferric heme]]
	[[Category: Lipocalin]]		[[Category: Lipocalin]]
			[[Category: Transport protein]]
	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 22:13:26 2009''~~