2a4d - Revision history

OCA at 07:18, 23 August 2023

2023-08-23T07:18:55Z

← Older revision		Revision as of 07:18, 23 August 2023
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	<StructureSection load='2a4d' size='340' side='right'caption='[[2a4d]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='2a4d' size='340' side='right'caption='[[2a4d]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A4D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A4D FirstGlance]. <br>
	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>UBE2V1, CROC1, UBE2V, UEV1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=9606 HUMAN])</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.69Å</td></tr>
	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class~~="~~sblockDat~~">~~<span class='plainlinks'>[https://en.wikipedia.org/wiki/Ubiquitin~~-~~-protein_ligase Ubiquitin--protein ligase]~~, ~~with EC number~~ [~~https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [https://pdbe.org/2a4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [https://www.ebi.ac.uk/pdbsum/2a4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a4d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [https://pdbe.org/2a4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [https://www.ebi.ac.uk/pdbsum/2a4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a4d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN]] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>		[https://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[~~Ubiquitin~~ conjugating enzyme\|~~Ubiquitin~~ conjugating enzyme]]		*[[3D structures of ubiquitin conjugating enzyme\|3D structures of ubiquitin conjugating enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Ubiquitin--protein ligase]]~~		[[Category: Arrowsmith C]]
	[[Category: Arrowsmith, C]]		[[Category: Avvakumov GV]]
	[[Category: Avvakumov~~, G V~~]]		[[Category: Bochkarev A]]
	[[Category: Bochkarev, A]]		[[Category: Dhe-Paganon S]]
	[[Category: Dhe-Paganon, S]]		[[Category: Edwards A]]
	[[Category: Edwards, A]]		[[Category: Mackenzie F]]
	[[Category: Mackenzie, F]]		[[Category: Newman EM]]
	[[Category: Newman~~, E M]]~~		[[Category: Sundstrom M]]
	~~[[Category: Structural genomic~~]]		[[Category: Walker JR]]
	[[Category: Sundstrom, M]]		[[Category: Weigelt J]]
	[[Category: Walker~~, J R~~]]		[[Category: Xue S]]
	[[Category: Weigelt, J]]
	[[Category: Xue, S]]
	~~[[Category: Alternative splicing]]~~
	~~[[Category: Ligase]]~~
	~~[[Category: Nuclear protein]]~~
	~~[[Category: Sgc]]~~
	~~[[Category: Ubiquitin]]~~
	~~[[Category: Ubiquitin- conjugating enzyme]]~~
	~~[[Category: Ubl conjugation pathway~~]]

OCA at 08:25, 27 January 2021

2021-01-27T08:25:28Z

← Older revision		Revision as of 08:25, 27 January 2021
Line 1:		Line 1:

	==Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)==		==Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)==
	<StructureSection load='2a4d' size='340' side='right' caption='[[2a4d]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='2a4d' size='340' side='right'caption='[[2a4d]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a4d]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2A4D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A4D FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [~~http~~://pdbe.org/2a4d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2a4d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2a4d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [https://pdbe.org/2a4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [https://www.ebi.ac.uk/pdbsum/2a4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a4d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN]] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>		[[https://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN]] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
	[[Category: Arrowsmith, C]]		[[Category: Arrowsmith, C]]

OCA at 07:13, 9 May 2018

2018-05-09T07:13:25Z

← Older revision		Revision as of 07:13, 9 May 2018
Line 1:		Line 1:

	==Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)==		==Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)==
	<StructureSection load='2a4d' size='340' side='right' caption='[[2a4d]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='2a4d' size='340' side='right' caption='[[2a4d]], [[Resolution\|resolution]] 1.69Å' scene=''>
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	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [http://pdbe.org/2a4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [http://www.ebi.ac.uk/pdbsum/2a4d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [http://pdbe.org/2a4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [http://www.ebi.ac.uk/pdbsum/2a4d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2a4d ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/2a4d_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/2a4d_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:32, 8 February 2016

2016-02-08T13:32:09Z

← Older revision		Revision as of 13:32, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a4d ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:03, 10 September 2015

2015-09-10T12:03:04Z

← Older revision		Revision as of 12:03, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2a4d' size='340' side='right' caption='[[2a4d]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='2a4d' size='340' side='right' caption='[[2a4d]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A4D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A4D FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [http://www.ebi.ac.uk/pdbsum/2a4d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [http://pdbe.org/2a4d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [http://www.ebi.ac.uk/pdbsum/2a4d PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2a4d" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Homo sapiens~~]]		[[Category: Human]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
	[[Category: Arrowsmith, C]]		[[Category: Arrowsmith, C]]

OCA at 23:41, 24 December 2014

2014-12-24T23:41:15Z

← Older revision		Revision as of 23:41, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2a4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A4D FirstGlance]. <br>		<table><tr><td colspan='2'>[[2a4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A4D FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [http://www.ebi.ac.uk/pdbsum/2a4d PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [http://www.ebi.ac.uk/pdbsum/2a4d PDBsum]</span></td></tr>
	<table>		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/UB2V1_HUMAN UB2V1_HUMAN]] Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.<ref>PMID:9305758</ref> <ref>PMID:9418904</ref> <ref>PMID:11057907</ref> <ref>PMID:9580084</ref> <ref>PMID:9705497</ref> <ref>PMID:20061386</ref> <ref>PMID:21512573</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
	[[Category: Arrowsmith, C.]]		[[Category: Arrowsmith, C]]
	[[Category: Avvakumov, G V.]]		[[Category: Avvakumov, G V]]
	[[Category: Bochkarev, A.]]		[[Category: Bochkarev, A]]
	[[Category: Dhe-Paganon, S.]]		[[Category: Dhe-Paganon, S]]
	[[Category: Edwards, A.]]		[[Category: Edwards, A]]
	[[Category: Mackenzie, F.]]		[[Category: Mackenzie, F]]
	[[Category: Newman, E M.]]		[[Category: Newman, E M]]
	[[Category: ~~SGC,~~ Structural ~~Genomics Consortium.~~]]		[[Category: Structural genomic]]
	[[Category: Sundstrom, M.]]		[[Category: Sundstrom, M]]
	[[Category: Walker, J R.]]		[[Category: Walker, J R]]
	[[Category: Weigelt, J.]]		[[Category: Weigelt, J]]
	[[Category: Xue, S.]]		[[Category: Xue, S]]
	[[Category: Alternative splicing]]		[[Category: Alternative splicing]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Nuclear protein]]		[[Category: Nuclear protein]]
	[[Category: Sgc]]		[[Category: Sgc]]
	~~[[Category: Structural genomic]]~~
	~~[[Category: Structural genomics consortium]]~~
	[[Category: Ubiquitin]]		[[Category: Ubiquitin]]
	[[Category: Ubiquitin- conjugating enzyme]]		[[Category: Ubiquitin- conjugating enzyme]]
	[[Category: Ubl conjugation pathway]]		[[Category: Ubl conjugation pathway]]

OCA at 03:38, 29 September 2014

2014-09-29T03:38:21Z

← Older revision		Revision as of 03:38, 29 September 2014
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	[[Image:~~2a4d~~.~~png~~\|~~left~~\|~~200px~~]]		==Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)==
			<StructureSection load='2a4d' size='340' side='right' caption='[[2a4d]], [[Resolution\|resolution]] 1.69Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2a4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A4D FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBE2V1, CROC1, UBE2V, UEV1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
			<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span></td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a4d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2a4d RCSB], [http://www.ebi.ac.uk/pdbsum/2a4d PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/2a4d_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Here we describe a systematic structure-function analysis of the human ubiquitin (Ub) E2 conjugating proteins, consisting of the determination of 15 new high-resolution three-dimensional structures of E2 catalytic domains, and autoubiquitylation assays for 26 Ub-loading E2s screened against a panel of nine different HECT (homologous to E6-AP carboxyl terminus) E3 ligase domains. Integration of our structural and biochemical data revealed several E2 surface properties associated with Ub chain building activity; (1) net positive or neutral E2 charge, (2) an "acidic trough" located near the catalytic Cys, surrounded by an extensive basic region, and (3) similarity to the previously described HECT binding signature in UBE2L3 (UbcH7). Mass spectrometry was used to characterize the autoubiquitylation products of a number of functional E2-HECT pairs, and demonstrated that HECT domains from different subfamilies catalyze the formation of very different types of Ub chains, largely independent of the E2 in the reaction. Our data set represents the first comprehensive analysis of E2-HECT E3 interactions, and thus provides a framework for better understanding the molecular mechanisms of ubiquitylation.

	~~{{STRUCTURE_2a4d\| PDB=2a4d \| SCENE= }}~~		A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.,Sheng Y, Hong JH, Doherty R, Srikumar T, Shloush J, Avvakumov GV, Walker JR, Xue S, Neculai D, Wan JW, Kim SK, Arrowsmith CH, Raught B, Dhe-Paganon S Mol Cell Proteomics. 2012 Aug;11(8):329-41. Epub 2012 Apr 10. PMID:22496338<ref>PMID:22496338</ref>

	~~===Structure~~ of the ~~human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>

	~~{{ABSTRACT_PUBMED_22496338}}~~		==See Also==
			*[[Ubiquitin conjugating enzyme\|Ubiquitin conjugating enzyme]]
	==~~About this Structure~~==		== References ==
	[[~~2a4d~~]] ~~is a 1 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA].~~		<references/>
			__TOC__
			</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]

OCA: Protected "2a4d" [edit=sysop:move=sysop]

2012-11-28T14:30:02Z

Protected "2a4d" [edit=sysop:move=sysop]

← Older revision	Revision as of 14:30, 28 November 2012
(No difference)

OCA at 14:29, 28 November 2012

2012-11-28T14:29:59Z

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	[[Image:2a4d.png\|left\|200px]]		[[Image:2a4d.png\|left\|200px]]

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	{{STRUCTURE_2a4d\| PDB=2a4d \| SCENE= }}		{{STRUCTURE_2a4d\| PDB=2a4d \| SCENE= }}

	===Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)===		===Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)===

			{{ABSTRACT_PUBMED_22496338}}

	==About this Structure==		==About this Structure==
	~~2A4D~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA].		[[2a4d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA].
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
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	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Structural genomics consortium]]		[[Category: Structural genomics consortium]]
			[[Category: Ubiquitin]]
	[[Category: Ubiquitin- conjugating enzyme]]		[[Category: Ubiquitin- conjugating enzyme]]
	[[Category: Ubl conjugation pathway~~,ubiquitin~~]]		[[Category: Ubl conjugation pathway]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:35:25 2009''~~

OCA at 20:35, 16 February 2009

2009-02-16T20:35:27Z

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	{{STRUCTURE_2a4d\| PDB=2a4d \| SCENE= }}		{{STRUCTURE_2a4d\| PDB=2a4d \| SCENE= }}

	~~'''~~Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)~~'''~~		===Structure of the human ubiquitin-conjugating enzyme E2 variant 1 (UEV-1)===


	==About this Structure==		==About this Structure==
	2A4D is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA].		2A4D is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A4D OCA].
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	~~[[Category: Single protein]]~~
	[[Category: Ubiquitin--protein ligase]]		[[Category: Ubiquitin--protein ligase]]
	[[Category: Arrowsmith, C.]]		[[Category: Arrowsmith, C.]]
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	[[Category: Ubiquitin- conjugating enzyme]]		[[Category: Ubiquitin- conjugating enzyme]]
	[[Category: Ubl conjugation pathway,ubiquitin]]		[[Category: Ubl conjugation pathway,ubiquitin]]
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