2adn - Revision history

OCA at 08:17, 15 May 2024

2024-05-15T08:17:11Z

← Older revision		Revision as of 08:17, 15 May 2024
Line 1:		Line 1:

	==Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding==		==Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding==
	<StructureSection load='2adn' size='340' side='right'caption='[[2adn~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>		<StructureSection load='2adn' size='340' side='right'caption='[[2adn]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ADN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ADN FirstGlance]. <br>
	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[2adl\|2adl]]</div~~></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [https://pdbe.org/2adn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [https://www.ebi.ac.uk/pdbsum/2adn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2adn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [https://pdbe.org/2adn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [https://www.ebi.ac.uk/pdbsum/2adn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2adn ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/CCDA_ECOLI CCDA_ECOLI] Antitoxin component of a toxin-antitoxin (TA) module which inhibits the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Labile antitoxin with a half-life of about 1 hour in the presence of CcdB. Binds to and blocks the activity of CcdB; will also remove bound CcdB protein from the CcdB-GyrA complex by forming a CcdA-CcdB complex, a process termed rejuvenation. The N-terminal 36 residues are not required for rejuventation. Functions as a transcriptional corepressor for the ccdAB operon, repression also requires CcdB.<ref>PMID:6327993</ref> <ref>PMID:2651399</ref> <ref>PMID:6308648</ref> <ref>PMID:2615761</ref> <ref>PMID:1324324</ref> <ref>PMID:8604132</ref> <ref>PMID:19647513</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Keller, W]]		[[Category: Keller W]]
	[[Category: Khatai, L]]		[[Category: Khatai L]]
	[[Category: Madl, T]]		[[Category: Madl T]]
	[[Category: Oberer, M]]		[[Category: Oberer M]]
	[[Category: VanMelderen, L]]		[[Category: VanMelderen L]]
	[[Category: Zangger, K]]		[[Category: Zangger K]]
	~~[[Category: Dna binding protein]]~~
	~~[[Category: Ribbon-helix-helix~~]]

OCA at 06:45, 10 November 2021

2021-11-10T06:45:10Z

← Older revision		Revision as of 06:45, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='2adn' size='340' side='right'caption='[[2adn]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2adn' size='340' side='right'caption='[[2adn]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ADN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ADN FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2adl\|2adl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2adl\|2adl]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [~~http~~://pdbe.org/2adn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2adn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2adn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [https://pdbe.org/2adn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [https://www.ebi.ac.uk/pdbsum/2adn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2adn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 05:24, 13 February 2020

2020-02-13T05:24:04Z

← Older revision		Revision as of 05:24, 13 February 2020
Line 1:		Line 1:

	==Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding==		==Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding==
	<StructureSection load='2adn' size='340' side='right' caption='[[2adn]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2adn' size='340' side='right'caption='[[2adn]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADN FirstGlance]. <br>
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
			[[Category: Large Structures]]
	[[Category: Keller, W]]		[[Category: Keller, W]]
	[[Category: Khatai, L]]		[[Category: Khatai, L]]

OCA at 07:16, 9 May 2018

2018-05-09T07:16:32Z

← Older revision		Revision as of 07:16, 9 May 2018
Line 1:		Line 1:

	==Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding==		==Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding==
	<StructureSection load='2adn' size='340' side='right' caption='[[2adn]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2adn' size='340' side='right' caption='[[2adn]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADN FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2adl\|2adl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2adl\|2adl]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [http://pdbe.org/2adn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [http://www.ebi.ac.uk/pdbsum/2adn PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [http://pdbe.org/2adn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [http://www.ebi.ac.uk/pdbsum/2adn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2adn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ad/2adn_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ad/2adn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:59, 9 February 2016

2016-02-09T20:59:21Z

← Older revision		Revision as of 20:59, 9 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2adn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:58, 11 September 2015

2015-09-11T01:58:53Z

← Older revision		Revision as of 01:58, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2adn' size='340' side='right' caption='[[2adn]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='2adn' size='340' side='right' caption='[[2adn]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADN FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2adl\|2adl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2adl\|2adl]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [http://www.ebi.ac.uk/pdbsum/2adn PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [http://pdbe.org/2adn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [http://www.ebi.ac.uk/pdbsum/2adn PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2adn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Escherichia~~ coli]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Keller, W]]		[[Category: Keller, W]]
	[[Category: Khatai, L]]		[[Category: Khatai, L]]

OCA at 09:36, 8 January 2015

2015-01-08T09:36:22Z

← Older revision		Revision as of 09:36, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADN FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2adl\|2adl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2adl\|2adl]]</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [http://www.ebi.ac.uk/pdbsum/2adn PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [http://www.ebi.ac.uk/pdbsum/2adn PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Keller, W.]]		[[Category: Keller, W]]
	[[Category: Khatai, L.]]		[[Category: Khatai, L]]
	[[Category: Madl, T.]]		[[Category: Madl, T]]
	[[Category: Oberer, M.]]		[[Category: Oberer, M]]
	[[Category: VanMelderen, L.]]		[[Category: VanMelderen, L]]
	[[Category: Zangger, K.]]		[[Category: Zangger, K]]
	[[Category: Dna binding protein]]		[[Category: Dna binding protein]]
	[[Category: Ribbon-helix-helix]]		[[Category: Ribbon-helix-helix]]

OCA at 00:23, 30 September 2014

2014-09-30T00:23:50Z

← Older revision		Revision as of 00:23, 30 September 2014
Line 1:		Line 1:
	[[Image:~~2adn~~.~~png~~\|~~left~~\|~~200px~~]]		==Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding==
			<StructureSection load='2adn' size='340' side='right' caption='[[2adn]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ADN FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2adl\|2adl]]</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2adn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2adn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2adn RCSB], [http://www.ebi.ac.uk/pdbsum/2adn PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ad/2adn_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Toxin-antitoxin systems are highly abundant in plasmids and bacterial chromosomes. They ensure plasmid maintenance by killing bacteria that have lost the plasmid. Their expression is autoregulated at the level of transcription. Here, we present the solution structure of CcdA, the antitoxin of the ccd system, as a free protein (16.7 kDa) and in complex with its cognate DNA (25.3 kDa). CcdA is composed of two distinct and independent domains: the N-terminal domain, responsible for DNA binding, which establishes a new family of the ribbon-helix-helix fold and the C-terminal region, which is responsible for the interaction with the toxin CcdB. The C-terminal domain is intrinsically unstructured and forms a tight complex with the toxin. We show that CcdA specifically recognizes a 6 bp palindromic DNA sequence within the operator-promoter (OP) region of the ccd operon and binds to DNA by insertion of the positively charged N-terminal beta-sheet into the major groove. The binding of up to three CcdA dimers to a 33mer DNA of its operator-promoter region was studied by NMR spectroscopy, isothermal titration calorimetry and single point mutation. The highly flexible C-terminal region of free CcdA explains its susceptibility to proteolysis by the Lon ATP-dependent protease.

	~~{{STRUCTURE_2adn\| PDB=2adn \| SCENE= }}~~		Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA.,Madl T, Van Melderen L, Mine N, Respondek M, Oberer M, Keller W, Khatai L, Zangger K J Mol Biol. 2006 Nov 24;364(2):170-85. Epub 2006 Sep 1. PMID:17007877<ref>PMID:17007877</ref>

	~~===Solution structure~~ of the ~~bacterial antitoxin CcdA: Implications for DNA and toxin binding===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_17007877}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2adn]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:017007877<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Keller, W.]]		[[Category: Keller, W.]]

OCA: Protected "2adn" [edit=sysop:move=sysop]

2013-01-06T09:47:07Z

Protected "2adn" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:47, 6 January 2013
(No difference)

OCA at 09:47, 6 January 2013

2013-01-06T09:47:04Z

← Older revision		Revision as of 09:47, 6 January 2013
Line 1:		Line 1:
	~~{{Seed}}~~
	[[Image:2adn.png\|left\|200px]]		[[Image:2adn.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2adn", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2adn\| PDB=2adn \| SCENE= }}		{{STRUCTURE_2adn\| PDB=2adn \| SCENE= }}

	===Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding===		===Solution structure of the bacterial antitoxin CcdA: Implications for DNA and toxin binding===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_17007877}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 17007877 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_17007877}}		{{ABSTRACT_PUBMED_17007877}}

	==About this Structure==		==About this Structure==
	~~2ADN~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA].		[[2adn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ADN OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~17007877~~</ref><references group="xtra"/>		<ref group="xtra">PMID:017007877</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Keller, W.]]		[[Category: Keller, W.]]
Line 31:		Line 19:
	[[Category: VanMelderen, L.]]		[[Category: VanMelderen, L.]]
	[[Category: Zangger, K.]]		[[Category: Zangger, K.]]
			[[Category: Dna binding protein]]
	[[Category: Ribbon-helix-helix]]		[[Category: Ribbon-helix-helix]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 12:42:30 2009''~~