2b3f - Revision history

OCA at 09:15, 14 February 2024

2024-02-14T09:15:46Z

← Older revision		Revision as of 09:15, 14 February 2024
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	==Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose==		==Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose==
	<StructureSection load='2b3f' size='340' side='right'caption='[[2b3f]]' scene=''>		<StructureSection load='2b3f' size='340' side='right'caption='[[2b3f]], [[Resolution\|resolution]] 1.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B3F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b3f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B3F FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [https://pdbe.org/2b3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [https://www.ebi.ac.uk/pdbsum/2b3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3f ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.56Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [https://pdbe.org/2b3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [https://www.ebi.ac.uk/pdbsum/2b3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3f ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q72KX2_THET2 Q72KX2_THET2]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Thermus thermophilus HB27]]
	[[Category: Beese LS]]		[[Category: Beese LS]]
	[[Category: Changela A]]		[[Category: Changela A]]

OCA at 11:25, 10 February 2021

2021-02-10T11:25:41Z

← Older revision		Revision as of 11:25, 10 February 2021
Line 1:		Line 1:

	==Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose==		==Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose==
	<StructureSection load='2b3f' size='340' side='right' caption='[[2b3f]]~~, [[Resolution\|resolution]] 1.56Å~~' scene=''>		<StructureSection load='2b3f' size='340' side='right'caption='[[2b3f]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[2b3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet2 Thet2].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2B3F FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B3F FirstGlance]. <br>
	</td></tr>~~<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [https://pdbe.org/2b3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [https://www.ebi.ac.uk/pdbsum/2b3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3f ProSAT]</span></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3b\|2b3b]]</td></tr>~~
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>~~
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [~~http~~://pdbe.org/2b3f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2b3f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3f ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/2b3f_consurf.spt"</scriptWhenChecked>		<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/2b3f_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b3f ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b3f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Periplasmic binding proteins (PBPs) comprise a protein superfamily that is involved in prokaryotic solute transport and chemotaxis. These proteins have been used to engineer reagentless biosensors to detect natural or non-natural ligands. There is considerable interest in obtaining very stable members of this superfamily from thermophilic bacteria to use as robust engineerable parts in biosensor development. Analysis of the recently determined genome sequence of Thermus thermophilus revealed the presence of more than 30 putative PBPs in this thermophile. One of these is annotated as a glucose binding protein (GBP) based on its genetic linkage to genes that are homologous to an ATP-binding cassette glucose transport system, although the PBP sequence is homologous to periplasmic maltose binding proteins (MBPs). Here we present the cloning, over-expression, characterization of cognate ligands, and determination of the X-ray crystal structure of this gene product. We find that it is a very stable (apo-protein Tm value is 100(+/- 2) degrees C; complexes 106(+/- 3) degrees C and 111(+/- 1) degrees C for glucose and galactose, respectively) glucose (Kd value is 0.08(+/- 0.03) microM) and galactose (Kd value is 0.94(+/- 0.04) microM) binding protein. Determination of the X-ray crystal structure revealed that this T. thermophilus glucose binding protein (ttGBP) is structurally homologous to MBPs rather than other GBPs. The di or tri-saccharide ligands in MBPs are accommodated in long relatively shallow grooves. In the ttGBP binding site, this groove is partially filled by two loops and an alpha-helix, which create a buried binding site that allows binding of only monosaccharides. Comparison of ttGBP and MBP provides a clear example of structural adaptations by which the size of ligand binding sites can be controlled in the PBP super family.

	The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites.,Cuneo MJ, Changela A, Warren JJ, Beese LS, Hellinga HW J Mol Biol. 2006 Sep 15;362(2):259-70. Epub 2006 Aug 10. PMID:16904687<ref>PMID:16904687</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 2b3f" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Thet2~~]]		[[Category: Large Structures]]
	[[Category: Beese~~, L S~~]]		[[Category: Beese LS]]
	[[Category: Changela, A]]		[[Category: Changela A]]
	[[Category: Cuneo~~, M J~~]]		[[Category: Cuneo MJ]]
	[[Category: Hellinga~~, H W~~]]		[[Category: Hellinga HW]]
	[[Category: Warren~~, J J]]~~		[[Category: Warren JJ]]
	~~[[Category: Galactose]]~~
	~~[[Category: Gbp]]~~
	~~[[Category: Glucose]]~~
	~~[[Category: Periplasmic binding protein]]~~
	~~[[Category: Protein-carbohydrate complex]]~~
	~~[[Category: Sugar binding protein~~]]

OCA at 07:44, 12 October 2017

2017-10-12T07:44:48Z

← Older revision		Revision as of 07:44, 12 October 2017
Line 1:		Line 1:

	==Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose==		==Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose==
	<StructureSection load='2b3f' size='340' side='right' caption='[[2b3f]], [[Resolution\|resolution]] 1.56Å' scene=''>		<StructureSection load='2b3f' size='340' side='right' caption='[[2b3f]], [[Resolution\|resolution]] 1.56Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3b\|2b3b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3b\|2b3b]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [http://pdbe.org/2b3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [http://www.ebi.ac.uk/pdbsum/2b3f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [http://pdbe.org/2b3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [http://www.ebi.ac.uk/pdbsum/2b3f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2b3f ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b3f ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:29, 11 September 2015

2015-09-11T13:29:06Z

← Older revision		Revision as of 13:29, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2b3f' size='340' side='right' caption='[[2b3f]], [[Resolution\|resolution]] 1.56Å' scene=''>		<StructureSection load='2b3f' size='340' side='right' caption='[[2b3f]], [[Resolution\|resolution]] 1.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2b3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus_hb27 Thermus thermophilus hb27~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B3F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B3F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3b\|2b3b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3b\|2b3b]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 ~~Thermus thermophilus HB27~~])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [http://www.ebi.ac.uk/pdbsum/2b3f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [http://pdbe.org/2b3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [http://www.ebi.ac.uk/pdbsum/2b3f PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
			<div class="pdbe-citations 2b3f" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Thermus thermophilus hb27~~]]		[[Category: Thet2]]
	[[Category: Beese, L S]]		[[Category: Beese, L S]]
	[[Category: Changela, A]]		[[Category: Changela, A]]

OCA at 08:51, 8 January 2015

2015-01-08T08:51:48Z

← Older revision		Revision as of 08:51, 8 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2b3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B3F FirstGlance]. <br>		<table><tr><td colspan='2'>[[2b3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B3F FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene><br>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3b\|2b3b]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3b\|2b3b]]</td></tr>
	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 Thermus thermophilus HB27])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 Thermus thermophilus HB27])</td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [http://www.ebi.ac.uk/pdbsum/2b3f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [http://www.ebi.ac.uk/pdbsum/2b3f PDBsum]</span></td></tr>
	<table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Thermus thermophilus hb27]]		[[Category: Thermus thermophilus hb27]]
	[[Category: Beese, L S.]]		[[Category: Beese, L S]]
	[[Category: Changela, A.]]		[[Category: Changela, A]]
	[[Category: Cuneo, M J.]]		[[Category: Cuneo, M J]]
	[[Category: Hellinga, H W.]]		[[Category: Hellinga, H W]]
	[[Category: Warren, J J.]]		[[Category: Warren, J J]]
	[[Category: Galactose]]		[[Category: Galactose]]
	[[Category: Gbp]]		[[Category: Gbp]]

OCA at 23:46, 29 September 2014

2014-09-29T23:46:10Z

← Older revision		Revision as of 23:46, 29 September 2014
Line 1:		Line 1:
	[[Image:~~2b3f~~.~~png~~\|~~left~~\|~~200px~~]]		==Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose==
			<StructureSection load='2b3f' size='340' side='right' caption='[[2b3f]], [[Resolution\|resolution]] 1.56Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[2b3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B3F FirstGlance]. <br>
			</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene><br>
			<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b3b\|2b3b]]</td></tr>
			<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0328 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 Thermus thermophilus HB27])</td></tr>
			<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2b3f RCSB], [http://www.ebi.ac.uk/pdbsum/2b3f PDBsum]</span></td></tr>
			<table>
			== Evolutionary Conservation ==
			[[Image:Consurf_key_small.gif\|200px\|right]]
			Check<jmol>
			<jmolCheckbox>
			<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b3/2b3f_consurf.spt"</scriptWhenChecked>
			<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
			<text>to colour the structure by Evolutionary Conservation</text>
			</jmolCheckbox>
			</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
			<div style="clear:both"></div>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Periplasmic binding proteins (PBPs) comprise a protein superfamily that is involved in prokaryotic solute transport and chemotaxis. These proteins have been used to engineer reagentless biosensors to detect natural or non-natural ligands. There is considerable interest in obtaining very stable members of this superfamily from thermophilic bacteria to use as robust engineerable parts in biosensor development. Analysis of the recently determined genome sequence of Thermus thermophilus revealed the presence of more than 30 putative PBPs in this thermophile. One of these is annotated as a glucose binding protein (GBP) based on its genetic linkage to genes that are homologous to an ATP-binding cassette glucose transport system, although the PBP sequence is homologous to periplasmic maltose binding proteins (MBPs). Here we present the cloning, over-expression, characterization of cognate ligands, and determination of the X-ray crystal structure of this gene product. We find that it is a very stable (apo-protein Tm value is 100(+/- 2) degrees C; complexes 106(+/- 3) degrees C and 111(+/- 1) degrees C for glucose and galactose, respectively) glucose (Kd value is 0.08(+/- 0.03) microM) and galactose (Kd value is 0.94(+/- 0.04) microM) binding protein. Determination of the X-ray crystal structure revealed that this T. thermophilus glucose binding protein (ttGBP) is structurally homologous to MBPs rather than other GBPs. The di or tri-saccharide ligands in MBPs are accommodated in long relatively shallow grooves. In the ttGBP binding site, this groove is partially filled by two loops and an alpha-helix, which create a buried binding site that allows binding of only monosaccharides. Comparison of ttGBP and MBP provides a clear example of structural adaptations by which the size of ligand binding sites can be controlled in the PBP super family.

	~~{{STRUCTURE_2b3f\| PDB=2b3f \| SCENE= }}~~		The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites.,Cuneo MJ, Changela A, Warren JJ, Beese LS, Hellinga HW J Mol Biol. 2006 Sep 15;362(2):259-70. Epub 2006 Aug 10. PMID:16904687<ref>PMID:16904687</ref>

	~~===Thermus thermophilus Glucose~~/~~Galactose Binding Protein Bound With Galactose===~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
	~~{{ABSTRACT_PUBMED_16904687}}~~		== References ==
			<references/>
	~~==About this Structure==~~		__TOC__
	~~[[2b3f]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA].~~		</StructureSection>

	==~~Reference~~==
	<~~ref group="xtra">PMID:016904687<~~/~~ref~~><~~references group="xtra"~~/>
	[[Category: Thermus thermophilus hb27]]		[[Category: Thermus thermophilus hb27]]
	[[Category: Beese, L S.]]		[[Category: Beese, L S.]]

OCA: Protected "2b3f" [edit=sysop:move=sysop]

2013-01-06T09:16:09Z

Protected "2b3f" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:16, 6 January 2013
(No difference)

OCA at 09:16, 6 January 2013

2013-01-06T09:16:06Z

← Older revision		Revision as of 09:16, 6 January 2013
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	~~{{Seed}}~~
	[[Image:2b3f.png\|left\|200px]]		[[Image:2b3f.png\|left\|200px]]

	~~<!--~~
	~~The line below this paragraph, containing "STRUCTURE_2b3f", creates the "Structure Box" on the page.~~
	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~
	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~
	~~or leave the SCENE parameter empty for the default display.~~
	~~-->~~
	{{STRUCTURE_2b3f\| PDB=2b3f \| SCENE= }}		{{STRUCTURE_2b3f\| PDB=2b3f \| SCENE= }}

	===Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose===		===Thermus thermophilus Glucose/Galactose Binding Protein Bound With Galactose===


	~~<!--~~
	~~The line below this paragraph, {{ABSTRACT_PUBMED_16904687}}, adds the Publication Abstract to the page~~
	~~(as it appears on PubMed at http://www.pubmed.gov), where 16904687 is the PubMed ID number.~~
	~~-->~~
	{{ABSTRACT_PUBMED_16904687}}		{{ABSTRACT_PUBMED_16904687}}

	==About this Structure==		==About this Structure==
	~~2B3F~~ is a 6 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA].		[[2b3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:~~16904687~~</ref><references group="xtra"/>		<ref group="xtra">PMID:016904687</ref><references group="xtra"/>
	[[Category: Thermus thermophilus hb27]]		[[Category: Thermus thermophilus hb27]]
	[[Category: Beese, L S.]]		[[Category: Beese, L S.]]
Line 36:		Line 24:
	[[Category: Protein-carbohydrate complex]]		[[Category: Protein-carbohydrate complex]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]

	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 19 08:38:21 2009''~~

OCA at 05:38, 19 April 2009

2009-04-19T05:38:22Z

← Older revision		Revision as of 05:38, 19 April 2009
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	==About this Structure==		==About this Structure==
	2B3F is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus~~ Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA].		2B3F is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA].

	==Reference==		==Reference==
	<ref group="xtra">PMID:16904687</ref><references group="xtra"/>		<ref group="xtra">PMID:16904687</ref><references group="xtra"/>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus hb27]]
	[[Category: Beese, L S.]]		[[Category: Beese, L S.]]
	[[Category: Changela, A.]]		[[Category: Changela, A.]]
Line 35:		Line 35:
	[[Category: Periplasmic binding protein]]		[[Category: Periplasmic binding protein]]
	[[Category: Protein-carbohydrate complex]]		[[Category: Protein-carbohydrate complex]]
			[[Category: Sugar binding protein]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 23~~:11:13 2009''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 19 08:38:21 2009''

OCA at 21:11, 17 February 2009

2009-02-17T21:11:18Z

← Older revision		Revision as of 21:11, 17 February 2009
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	==About this Structure==		==About this Structure==
	2B3F is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA].		2B3F is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3F OCA].

	==Reference==		==Reference==
	The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites., Cuneo MJ, Changela A, Warren JJ, Beese LS, Hellinga HW, J Mol Biol. 2006 Sep 15;362(2):259-70. Epub 2006 Aug 10. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~16904687 16904687]~~		<ref group="xtra">PMID:16904687</ref><references group="xtra"/>
	~~[[Category: Single protein]]~~
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Beese, L S.]]		[[Category: Beese, L S.]]
Line 37:		Line 36:
	[[Category: Protein-carbohydrate complex]]		[[Category: Protein-carbohydrate complex]]

	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 06~~:52:~~32 2008~~''		''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 23:11:13 2009''